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Abstract:

Background: The use of microorganisms for the synthesis of nanoparticles (NPs) is relatively new in basic research and technology areas. Purpose: This work was conducted to optimized the biosynthesis of iron NPs intra- and extracellular by Escherichia coli or Pseudomonas aeruginosa and to evaluate their anticoagulant activity. Study Design/Methods: The structures and properties of the iron NPs were investigated by Ultraviolet–visible (UV-vis) spectroscopy, Zeta potential, Dynamic light scattering (DLS), Field emission scanning electron microscope (FESEM)/ Energy dispersive X-ray (EDX) and transmission electron microscopy (TEM). Anticoagulant activity was determined by conducting trials of Thrombin Time (TT), Activated Partial Prothrombin Time (APTT) and Prothrombin Time (PT). Results: UV-vis spectrum of the aqueous medium containing iron NPs showed a peak at 275 nm. The forming of iron NPs was confirmed by FESEM/ EDX, and TEM. The morphology was spherical shapes mostly with low polydispersity and the average particle diameter was 23 ± 1 nm. Iron NPs showed anticoagulant activity by the activation of extrinsic pathway. Conclusion: The eco-friendly process of biosynthesis of iron NPs employing prokaryotic microorganisms presents a good anticoagulant activity. This could be explored as promising candidates for a variety of biomedical and pharmaceutical applications. © 2016, Springer Science+Business Media New York.

Registro:

Documento: Artículo
Título:Intra- and Extracellular Biosynthesis and Characterization of Iron Nanoparticles from Prokaryotic Microorganisms with Anticoagulant Activity
Autor:Crespo, K.A.; Baronetti, J.L.; Quinteros, M.A.; Páez, P.L.; Paraje, M.G.
Filiación:Instituto Multidisciplinario de Biología Vegetal (IMBIV) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Cátedra de Microbiología, Facultad de Ciencias Exactas Físicas y Naturales, Universidad Nacional de Córdoba (UNC), Av. Vélez Sarsfield 299, Córdoba, Argentina
Unidad de Tecnología Farmacéutica (UNITEFA) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba (UNC), Córdoba, Argentina
Palabras clave:anticoagulant activity; Escherichia coli; iron nanoparticles; microbial biosynthesis; Pseudomonas aeruginosa
Año:2017
Volumen:34
Número:3
Página de inicio:591
Página de fin:598
DOI: http://dx.doi.org/10.1007/s11095-016-2084-0
Título revista:Pharmaceutical Research
Título revista abreviado:Pharm. Res.
ISSN:07248741
CODEN:PHREE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07248741_v34_n3_p591_Crespo

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Citas:

---------- APA ----------
Crespo, K.A., Baronetti, J.L., Quinteros, M.A., Páez, P.L. & Paraje, M.G. (2017) . Intra- and Extracellular Biosynthesis and Characterization of Iron Nanoparticles from Prokaryotic Microorganisms with Anticoagulant Activity. Pharmaceutical Research, 34(3), 591-598.
http://dx.doi.org/10.1007/s11095-016-2084-0
---------- CHICAGO ----------
Crespo, K.A., Baronetti, J.L., Quinteros, M.A., Páez, P.L., Paraje, M.G. "Intra- and Extracellular Biosynthesis and Characterization of Iron Nanoparticles from Prokaryotic Microorganisms with Anticoagulant Activity" . Pharmaceutical Research 34, no. 3 (2017) : 591-598.
http://dx.doi.org/10.1007/s11095-016-2084-0
---------- MLA ----------
Crespo, K.A., Baronetti, J.L., Quinteros, M.A., Páez, P.L., Paraje, M.G. "Intra- and Extracellular Biosynthesis and Characterization of Iron Nanoparticles from Prokaryotic Microorganisms with Anticoagulant Activity" . Pharmaceutical Research, vol. 34, no. 3, 2017, pp. 591-598.
http://dx.doi.org/10.1007/s11095-016-2084-0
---------- VANCOUVER ----------
Crespo, K.A., Baronetti, J.L., Quinteros, M.A., Páez, P.L., Paraje, M.G. Intra- and Extracellular Biosynthesis and Characterization of Iron Nanoparticles from Prokaryotic Microorganisms with Anticoagulant Activity. Pharm. Res. 2017;34(3):591-598.
http://dx.doi.org/10.1007/s11095-016-2084-0