Artículo

Ferreira, M.L.; Casabuono, A.C.; Stacchiotti, S.T.; Couto, A.S.; Ramirez, S.A.; Vullo, D.L. "Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes" (2017) International Biodeterioration and Biodegradation. 119:605-613
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Abstract:

The production, isolation, purification and characterization of soluble Extracellular Polymeric Substances (EPS) secreted by Pseudomonas veronii 2E were studied. Optimal production was obtained in medium M9-14 g l−1 glycerol, 25 °C. Spectrophotometric and potentiometric titration studies showed that EPS consisted on extracellular DNA, proteins and polysaccharides as the main components. DNase I, Proteinase K and further sodium deoxycholate treatments yielded similar proportions of lipopolysaccharides (LPSs) and exopolysaccharides (ExPs). ExP was subjected to total acid hydrolysis and then analyzed by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD), showing the presence of fucose, galactosamine, glucosamine, galactose, glucose, mannose and glucuronic acid. Further treatment with oxalic acid confirmed the presence of a pyruvilated sugar. The ability of the secreted products to interact with Cd(II) was studied by anodic stripping voltammetry (ASV). LPS fraction contained the highest ligand concentration (279 ± 10 μmoles Cd(II) binding sites g LPS−1). This information is a valuable tool for the application of EPS in industrial wastewater treatment. © 2016 Elsevier Ltd

Registro:

Documento: Artículo
Título:Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes
Autor:Ferreira, M.L.; Casabuono, A.C.; Stacchiotti, S.T.; Couto, A.S.; Ramirez, S.A.; Vullo, D.L.
Filiación:Área Química, Instituto de Ciencias, Universidad Nacional de General Sarmiento, J.M. Gutierrez 1150, Los Polvorines, Buenos Aires B1613GSX, Argentina
Consejo Nacional de lnvestigaciones Científicas y Técnicas, Av. Rivadavia 1917, Buenos Aires, C1033AAJ, Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Orgánica - Consejo Nacional de lnvestigaciones Cientificas y Técnicas, Centro de Investigación en Hidratos de Carbono (CIHIDECAR), Buenos Aires, Argentina
Palabras clave:Bacterial lipopolysaccharide (LPS); Biotreatment; Cadmium retention; Extracellular polymeric substances (EPS); Pseudomonas veronii 2E; Bacteria; Binding sites; Chromatography; Glucose; Industrial water treatment; Ligands; Oxalic acid; Polymers; Polysaccharides; Titration; Voltammetry; Anodic stripping voltammetry; Biotreatments; Extracellular polymeric substances; High-performance anion-exchange chromatography; Industrial wastewater treatment; Lipopolysaccharides; Pseudomonas veronii; Pulsed amperometric detection; Wastewater treatment; bacterium; bioactivity; cadmium; chemical analysis; concentration (composition); exopolymer; hydrolysis; ligand; oxalic acid; polysaccharide; purification; waste treatment; wastewater treatment; Bacteria (microorganisms); Pseudomonas veronii
Año:2017
Volumen:119
Página de inicio:605
Página de fin:613
DOI: http://dx.doi.org/10.1016/j.ibiod.2016.10.013
Título revista:International Biodeterioration and Biodegradation
Título revista abreviado:Int. Biodeterior. Biodegrad.
ISSN:09648305
CODEN:IBBIE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09648305_v119_n_p605_Ferreira

Referencias:

  • Barkleit, A., Moll, H., Bernhard, G., Interaction of uranium(VI) with lipopolysaccharide (2008) Dalton Trans., pp. 2879-2886
  • Bao, Z., Chen, X., Zhao, J., Lin, F., Li, J., Zhang, Y., Exploring the relationship between the EPS property and the toxicity of sludge for treating 4-chlorophenol synthetic wastewater in a sequencing batch reactor (2016) Int. Biodeterior. Biodegrad., 110, pp. 24-31
  • Blumenkrantz, N., Asboe-Hansen, G., New method for quantitative determination of uronic acids (1973) Anal. Biochem., 54, pp. 484-489
  • Braissat, O., Decho, A., Dupraz, C., Glunk, C., Przekop, K., Visscher, P., Exopolymeric substances of sulfate-reducing bacteria: interactions with calcium at alkaline pH and implication for formation of carbonate minerals (2007) Geobiology, 5, pp. 1-11
  • Bray, R.H., Kurtz, L.T., Determination of total, organic and available forms of phosphorus in soils (1945) Soil Sci., 59, pp. 39-46
  • Cavallero, G., Casabuono, A.C., Ferreira, M.L., Vullo, D.L., Couto, A.S., Caracterización del exopolisacárido de Pseudomonas veronii 2E (2015), p. 86. , Proceedings XX Simposio Nacional de Química Orgánica Sociedad Argentina de Investigación en Química Orgánica, p; Celik, G.Y., Aslim, B., Beyatli, Y., Characterisation and production of exopolysaccharide (EPS) from Pseudomonas aeruginosa G1 and Pseudomonas putida G12 strains (2008) Carbohydr. Polym., 73, pp. 178-182
  • Ceretti, H.M., Vullo, D.L., Zalts, A., Ramírez, S., Effect of bacterial growth in the complexing capacity of a culture medium supplemented with cadmium(II) (2010) World J. Microbiol. Biotechnol., 26, pp. 847-853
  • Chen, X., Santschi, P.H., Schwehr, K.A., Hung, C.C., Optimized isolation procedure for obtaining strongly actinide binding exopolymeric substances (EPS) from two bacteria (Sagittula stellata and Pseudomonas fluorescens Biovar II) (2009) Bioresour. Technol., 100, pp. 6010-6021
  • Comte, S., Guibaud, G., Baudu, M., Relations between extraction protocols for activated sludge extracellular polymeric substances (EPS) and EPS complexation properties. Part I. Comparison of the efficiency of eight EPS extraction methods (2006) Enzyme Microb. Technol., 38, pp. 237-245
  • Comte, S., Guibaud, G., Baudu, M., Biosorption properties of extracellular polymeric substances (EPS) towards Cd, Cu and Pb for different pH values (2008) J. Hazard. Mater., 151, pp. 185-193
  • Comte, S., Guibaud, G., Baudu, M., Relations between extraction protocols for activated sludge extracellular polymeric substances (EPS) and complexation properties of Pb and Cd with EPS Part II. Consequences of EPS extraction methods on Pb2+ and Cd2+ complexation (2006) Enzyme Microb. Technol., 38, pp. 246-252
  • Comte, S., Guibaud, G., Baudu, M., Biosorption properties of extracellular polymeric subtances (EPS) resulting from activated sludge according to their type: soluble or bound (2006) Process Biochem., 41, pp. 815-823
  • D'Abzac, P., Bordas, F., van Hullebusch, E., Lens, P., Guibaud, G., Extraction of extracellular polymeric substances (EPS) from anaerobic granular sludges: comparison of chemical and physical extraction protocols (2010) Appl. Microbiol. Biotechnol., 85, pp. 1589-1599
  • D'Abzac, P., Bordas, F., van Hullebusch, E., Lens, P., Guibaud, G., Effects of extraction procedures on metal binding properties of extracellular polymeric substances (EPS) from anaerobic granular sludges (2010) Colloids Surfaces B Biointerfaces, 80, pp. 161-168
  • Daniel, M.A., Barrionuevo, M., Doyle, S., Vullo, D.L., Kinetics of Pseudomonas veronii 2E biofilm development under under different nutritional conditions for a proper bioreactor design (2016) Biochem. Eng. J., 105, pp. 150-158
  • Fang, H.H.P., Jia, X.S., Extraction of extracelullar polymeric from anaerobic sludges (1996) Biotechnol. Tech., 10, pp. 803-808
  • Farooq, U., Kozinski, J., Khan, M., Athar, M., Biosorption of heavy metal ions using wheat based biosorbents-a review of the recent literature (2010) Bioresour. Technol., 101, pp. 5043-5053
  • Filisetti-Cozzi, T., Carpita, N., Measurement of uronic acids without interference from neutral sugars (1991) Anal. Biochem., 197, pp. 157-162
  • Flemming, H.C., Wingender, J., The biofilm matrix (2010) Nat. Rev. Microbiol., 8, pp. 623-633
  • Freitas, F., Alves, D., Pais, J., Costa, N., Olivera, C., Mafra, L., Reis, M., Characterization of an extracellular polysaccharide produced by Pseudomonas strain grown on glycerol (2009) Bioresour. Technol., 100, pp. 859-865
  • Freitas, F., Alves, V.D., Reis, M.A., Advances in bacterial exopolysaccharides: from production to biotechnological applications (2011) Trends Biotechnol., 29, pp. 388-398
  • Frølund, B., Palmgren, R., Keiding, K., Nielsen, P., Extraction of extracellular polymer from activated sludge using cation exchange resin (1996) Water Res., 30, pp. 1749-1758
  • Gadd, G., Metals, minerals and microbes: geomicrobiology and bioremediation (2010) Microbiology, 156, pp. 609-643
  • Garavaglia, L., Cerdeira, S., Vullo, D.L., Chromium (VI) biotransformation by beta- and gamma-Proteobacteria from natural polluted environments: a combined biological and chemical treatment for industrial wastes (2010) J. Hazard. Mater., 15, pp. 104-110
  • Geyik, A.G., Çeçen, F., Variations in extracellular polymeric substances (EPS) during adaptation of activated sludges to new feeding conditions (2015) Int. Biodeterior. Biodegrad., 105, pp. 137-145
  • Guard-Petter, J., Lakshmi, B., Carlson, R., Ingram, K., On-farm monitoring of mouse-invasive Salmonella enterica serovar enteritidis and a model for its association with the production of contaminated eggs (1995) Appl. Environ. Microbiol., 61, pp. 2845-2851
  • Guibaud, G., Bordas, F., Saaid, A., D'Abzac, P., van Hullenbusch, E., Effect of pH on cadmium and lead binding by extracellular polymeric subtances (EPS) extracted from environmental bacterial strains (2008) Colloids Surfaces, 63, pp. 48-54
  • Guibaud, G., Comte, S., Bordas, F., Dupuy, S., Baudu, M., Comparison of the complexation potential of extracellular polymeric substances (EPS), extracted from activated sludges and produced by pure bacteria strains, for cadmium, lead and nickel (2005) Chemosphere, 59, pp. 629-638
  • Guibaud, G., Tixer, N., Bouju, A., Baudu, M., Relation between extracellular polymers' composition and its ability to complex Cd, Cu and Pb (2003) Chemosphere, 52, pp. 1701-1710
  • Guibaud, G., van Hullebusch, E., Bordas, F., Lead and cadmium biosorption by extracellular polymeric subtances (EPS) extracted from activated sludges: pH-sorption edge and mathematical equilibrium modelling (2006) Chemosphere, 64, pp. 1955-1962
  • Kachlany, S.C., Levery, S., Kim, J., Reuhs, B., Lion, L., Ghiorse, W., Structure and carbohydrate analysis of the exopolysaccharide capsule of Pseudomonas putida G7 (2001) Environ. Microbiol., 3, pp. 774-784
  • Klock, J.H., Wieland, A., Seifert, R., Extracellular polymeric substances (EPS) from cyanobacterial mats: characterisation and isolation method optimisation (2007) Mar. Biol., 152, pp. 1077-1085
  • Krauss, J., Weckesser, J., Mayer, H., Structural analysis of the nontoxic lipid A of Rhodobacter capsulatus 37b4 (1988) Eur. J. Biochem., 38, pp. 157-163
  • Langley, S., Beveridge, T., Effect of O-side-chain-lipopolysaccharide chemistry on metal binding (1999) Appl. Environ. Microbiol., 65, pp. 489-498
  • Li, Z., Tian, Y., Ding, Y., Wang, H., Chen, L., Contribution of extracellular polymeric substances (EPS) and their subfractions to the sludge aggregation in membrane bioreactor coupled with worm reactor (2013) Bioresour. Technol., 144, pp. 328-336
  • Liu, H., Fang, H.H.P., Influences of extracellular polymeric substances (EPS) on flocculation, settling, and dewatering of activated sludge (2003) Crit. Rev. Environ. Sci. Technol., 33, pp. 237-273
  • Lloyd, J.R., Lovley, D.R., Microbial detoxification of metals and radionuclides (2001) Curr. Opin. Biotechnol., 12, pp. 248-253
  • Loaëc, M., Olier, R., Guezennec, J., Uptake of lead, cadmium and zinc by a novel bacterial exopolysaccharide (1997) Water Res., 31, pp. 1171-1179
  • McSwain, B.S., Irvine, R.L., Hausner, M., Wilderer, P.A., Composition and distribution of extracellular polymeric substances in aerobic flocs and granular sludge (2005) Appl. Environ. Microbiol., 71, pp. 1051-1057
  • Martinez, R., Smith, D., Kulczycki, E., Ferris, F., Determination of intrinsic bacterial surface acidity constants using a Donnan Shell model and a continuous pKa distribution method (2002) Methods Surf. Charact., 253, pp. 130-139
  • Meisen, S., Wingender, J., Telgheder, U., Analysis of microbial extracellular polysaccharides in biofilms by HPLC. Part I: development of the analytical method using two complementary stationary phases (2008) Anal. Bioanal. Chem., 391, pp. 993-1002
  • Mendez, N., Ramírez, S., Ceretti, H., Zalts, A., Candal, R., Vullo, D.L., Pseudomonas veronii 2E surface interactions with Zn(II) and Cd(II) (2011) Glob. J. Environ. Sci. Technol., 1, p. 3
  • More, T., Yadav, J.Y., Tyagi, R., Surampalli, R., Extracellular polymeric substances of bacterial and their potential environmental applications (2014) Environ. Manag., 144, pp. 1-25
  • Morlay, C., Cromer, M., Mouginot, Y., Vittori, O., Potentiometric study of Cu(II) and Ni(II) complexation with two high molecular weight poly(acrylic acids) (1998) Talanta, 45, pp. 1177-1188
  • Nielsen, P., Jahn, A., Extraction of EPS (1999) Microbial Polymeric Substances: Characterization, Structure and Function (1st ed.), pp. 49-72. , J. Wingender T. Neu H. Flemming Springer-Verlag
  • Oliveira, M.R., Ferreira da Silva, R.S., Buzato, J.B., Calabone Celligoi, M.A., Study of levan production by Zymomonas mobilis using regional low-cost carbohydrate sources (2007) Biochem. Eng. J., 37, pp. 177-183
  • Pal, A., Paul, A.K., Microbial extracellular polymeric substances: central elements in heavy metal bioremediation (2008) Indian J. Microbiol., 48, pp. 49-64
  • Pan, X., Liu, J., Song, W., Zhang, D., Biosorption of Cu(II) to extracellular polymeric substances (EPS) from Synechoeystis sp.: a fluorescence quenching study (2012) Front. Environ. Sci. Eng., 6, pp. 493-497
  • Reuhs, B., Geller, D., Kim, J., Fox, J., Kumar Kolli, V., Pueppk, S., Sinorhizobium fredii and Sinorhizobium meliloti produce structurally conserved lipopolysaccharides and strain-specific K antigens (1998) Appl. Environ. Microbiol., 64, pp. 4930-4938
  • Ružić, I., Trace metal complexation at herogeneous binding sites in aquatic systems (1996) Mar. Chem., 53, pp. 1-15
  • Scarponi, G., Capodaglio, G., Barbante, C., Cescon, P., The anodic stripping voltammetric titration procedure for study of trace metal complexation in seawater (1996) Element Speciation in Bioinorganic Chemistry, pp. 363-418. , S. Caroli Wiley New York
  • Scatchard, G., The attractions of proteins for small molecules and ions (1949) Ann. N. Y. Acad. Sci., 51, pp. 660-672
  • Sedmak, J., Grosserberg, S., A rapid, sensitive, and versatile assay for protein using Coomassie Brilliant Blue G250 (1977) Anal. Biochem., 79, pp. 544-552
  • Silva, M.F., Fornari, R.C.G., Mazutti, M.A., Oliveira, D., Ferreira Padilha, F., Cichoski, A.J., Cansian, R.L., Treichel, H., Production and characterization of xanthan gum by Xanthomonas campestris using cheese whey as sole carbon source (2009) J. Food Eng., 90, pp. 119-123
  • Sutherland, I.W., Microbial exopolysaccharides-structural subtleties and their consequences (1997) Pure Appl. Chem., 69, pp. 1911-1917
  • Sutherland, I.W., Microbial polyssaccharides from Gram-negative bacteria (2001) Int. Dairy J., 11, pp. 663-674
  • Tsai, C.M., Frasch, C.E., A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels (1982) Anal. Biochem., 119, pp. 115-119
  • Valls, M., de Lorenzo, V., Exploiting the genetic and biochemical capacities of bacteria for the remediation of heavy metal pollution (2002) FEMS Microbiol. Rev., 26, pp. 327-338
  • Vullo, D.L., Ceretti, H.M., Daniel, M.A., Ramírez, S.A., Zalts, A., Cadmium, zinc and copper biosorption mediated by Pseudomonas veronii 2E (2008) Bioresour. Technol., 99, pp. 5574-5581
  • Weia, X., Fang, L., Cai, P., Huang, Q., Chen, H., Liang, W., Rong, X., Influence of extracellular polymeric substances (EPS) on Cd adsorption by bacteria (2011) Environ. Pollut., 159, pp. 1369-1374
  • Wingerder, J., Neu, T.R., Flemming, H.C., What are bacterial extracellular polymeric substances? (1999) Microbial Polymeric Substances: Characterization, Structure and Function (1st ed.), pp. 1-19. , J. Wingender T. Neu H. Flemming Springer-Verlag
  • Xuan, W., Bin, Z., Zhiqianq, S., Zhiqianq, Q., Zhaoli, C., Min, J., Junwen, L., Jinqfenq, W., The EPS characteristics of sludge in an aerobic granule membrane bioreactor (2010) Bioresour. Technol., 101, pp. 8046-8050
  • Yee, N., Fein, J., Cd adsorption onto bacterial surfaces: a universal adsorption edge? (2001) Geochimica Cosmochimica Acta, 65, pp. 2037-2042
  • Yin, Y., Hu, Y., Xiong, F., Sorption of Cu(II) and Cd(II) by extracellular polymeric substances (EPS) from Aspergillus fumigatus (2011) Int. Biodeterior. Biodegrad., 65, pp. 1012-1018
  • Yokoi, H., Kawata, S., Iwaizumi, M., Interaction modes between heavy metal ions and water-soluble polymers. 2. Spectroscopic and magnetic reexamination of the aqueous solutions of cupric ions and poly(acrylic acid) (1986) J. Am. Chem. Soc., 108, pp. 3361-3365

Citas:

---------- APA ----------
Ferreira, M.L., Casabuono, A.C., Stacchiotti, S.T., Couto, A.S., Ramirez, S.A. & Vullo, D.L. (2017) . Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes. International Biodeterioration and Biodegradation, 119, 605-613.
http://dx.doi.org/10.1016/j.ibiod.2016.10.013
---------- CHICAGO ----------
Ferreira, M.L., Casabuono, A.C., Stacchiotti, S.T., Couto, A.S., Ramirez, S.A., Vullo, D.L. "Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes" . International Biodeterioration and Biodegradation 119 (2017) : 605-613.
http://dx.doi.org/10.1016/j.ibiod.2016.10.013
---------- MLA ----------
Ferreira, M.L., Casabuono, A.C., Stacchiotti, S.T., Couto, A.S., Ramirez, S.A., Vullo, D.L. "Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes" . International Biodeterioration and Biodegradation, vol. 119, 2017, pp. 605-613.
http://dx.doi.org/10.1016/j.ibiod.2016.10.013
---------- VANCOUVER ----------
Ferreira, M.L., Casabuono, A.C., Stacchiotti, S.T., Couto, A.S., Ramirez, S.A., Vullo, D.L. Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes. Int. Biodeterior. Biodegrad. 2017;119:605-613.
http://dx.doi.org/10.1016/j.ibiod.2016.10.013