Artículo

Muñoz, M.J.; Nieto Moreno, N.; Giono, L.E.; Cambindo Botto, A.E.; Dujardin, G.; Bastianello, G.; Lavore, S.; Torres-Méndez, A.; Menck, C.F.M.; Blencowe, B.J.; Irimia, M.; Foiani, M.; Kornblihtt, A.R. "Major Roles for Pyrimidine Dimers, Nucleotide Excision Repair, and ATR in the Alternative Splicing Response to UV Irradiation" (2017) Cell Reports. 18(12):2868-2879
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Abstract:

We have previously found that UV irradiation promotes RNA polymerase II (RNAPII) hyperphosphorylation and subsequent changes in alternative splicing (AS). We show now that UV-induced DNA damage is not only necessary but sufficient to trigger the AS response and that photolyase-mediated removal of the most abundant class of pyrimidine dimers (PDs) abrogates the global response to UV. We demonstrate that, in keratinocytes, RNAPII is the target, but not a sensor, of the signaling cascade initiated by PDs. The UV effect is enhanced by inhibition of gap-filling DNA synthesis, the last step in the nucleotide excision repair pathway (NER), and reduced by the absence of XPE, the main NER sensor of PDs. The mechanism involves activation of the protein kinase ATR that mediates the UV-induced RNAPII hyperphosphorylation. Our results define the sequence UV-PDs-NER-ATR-RNAPII-AS as a pathway linking DNA damage repair to the control of both RNAPII phosphorylation and AS regulation. © 2017 The Author(s)

Registro:

Documento: Artículo
Título:Major Roles for Pyrimidine Dimers, Nucleotide Excision Repair, and ATR in the Alternative Splicing Response to UV Irradiation
Autor:Muñoz, M.J.; Nieto Moreno, N.; Giono, L.E.; Cambindo Botto, A.E.; Dujardin, G.; Bastianello, G.; Lavore, S.; Torres-Méndez, A.; Menck, C.F.M.; Blencowe, B.J.; Irimia, M.; Foiani, M.; Kornblihtt, A.R.
Filiación:Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET) and Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Argentina
Fondazione Istituto FIRC di Oncologia Molecolare (IFOM), Via Adamello 16, Milan, 20139, Italy
Centre for Genomic Regulation, Barcelona Institute of Science and Technology (BIST), Dr. Aiguader 88, Barcelona, 08003, Spain
Universitat Pompeu Fabra (UPF), Barcelona, 08003, Spain
Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, 05508-900, Brazil
Donnelly Centre and Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 3E1, Canada
Palabras clave:alternative splicing; ATR; cyclobutane pyrimidine dimers; DNA damage; global genome repair; nucleotide excision repair; Potorous photolyase; UV irradiation; ATR protein; cyclobutane; deoxyribodipyrimidine photolyase; pyrimidine dimer; RNA polymerase II; ATM protein; ATR protein, human; DNA; pyrimidine dimer; RNA polymerase II; alternative RNA splicing; Article; controlled study; DNA damage; DNA repair; DNA synthesis; enzyme phosphorylation; excision repair; gene expression; human; human cell; inhibition kinetics; keratinocyte; signal transduction; transcription initiation; ultraviolet irradiation; alternative RNA splicing; cytology; DNA repair; genetic transcription; genetics; metabolism; phosphorylation; radiation response; skin; ultraviolet radiation; Alternative Splicing; Ataxia Telangiectasia Mutated Proteins; DNA; DNA Repair; Humans; Keratinocytes; Phosphorylation; Pyrimidine Dimers; RNA Polymerase II; Skin; Transcription, Genetic; Ultraviolet Rays
Año:2017
Volumen:18
Número:12
Página de inicio:2868
Página de fin:2879
DOI: http://dx.doi.org/10.1016/j.celrep.2017.02.066
Título revista:Cell Reports
Título revista abreviado:Cell Rep.
ISSN:22111247
CAS:cyclobutane, 287-23-0; deoxyribodipyrimidine photolyase, 37290-70-3; pyrimidine dimer, 25247-63-6; DNA, 9007-49-2; Ataxia Telangiectasia Mutated Proteins; ATR protein, human; DNA; Pyrimidine Dimers; RNA Polymerase II
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_22111247_v18_n12_p2868_Munoz

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Citas:

---------- APA ----------
Muñoz, M.J., Nieto Moreno, N., Giono, L.E., Cambindo Botto, A.E., Dujardin, G., Bastianello, G., Lavore, S.,..., Kornblihtt, A.R. (2017) . Major Roles for Pyrimidine Dimers, Nucleotide Excision Repair, and ATR in the Alternative Splicing Response to UV Irradiation. Cell Reports, 18(12), 2868-2879.
http://dx.doi.org/10.1016/j.celrep.2017.02.066
---------- CHICAGO ----------
Muñoz, M.J., Nieto Moreno, N., Giono, L.E., Cambindo Botto, A.E., Dujardin, G., Bastianello, G., et al. "Major Roles for Pyrimidine Dimers, Nucleotide Excision Repair, and ATR in the Alternative Splicing Response to UV Irradiation" . Cell Reports 18, no. 12 (2017) : 2868-2879.
http://dx.doi.org/10.1016/j.celrep.2017.02.066
---------- MLA ----------
Muñoz, M.J., Nieto Moreno, N., Giono, L.E., Cambindo Botto, A.E., Dujardin, G., Bastianello, G., et al. "Major Roles for Pyrimidine Dimers, Nucleotide Excision Repair, and ATR in the Alternative Splicing Response to UV Irradiation" . Cell Reports, vol. 18, no. 12, 2017, pp. 2868-2879.
http://dx.doi.org/10.1016/j.celrep.2017.02.066
---------- VANCOUVER ----------
Muñoz, M.J., Nieto Moreno, N., Giono, L.E., Cambindo Botto, A.E., Dujardin, G., Bastianello, G., et al. Major Roles for Pyrimidine Dimers, Nucleotide Excision Repair, and ATR in the Alternative Splicing Response to UV Irradiation. Cell Rep. 2017;18(12):2868-2879.
http://dx.doi.org/10.1016/j.celrep.2017.02.066