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Ver el documento (formato PDF)   Elola, María Teresa.  "Detección, cuantificación y caracterización de Lectinas ß-Galactosídicas de Bufo arenarum"  (1995)
Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires
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Abstract:
S-type lectins from Bufo arenarum ovary and blastula embryos (8-16 blastomeres) were purified. These proteins showed p-galactoside-binding specificity, Caw-independency and the requirement of thiol-reducing agents to maintain their hemagglutinating activity. Lectin activity showed to be developmentally regulated in Bufo arenarum embryos. Subunit molecular weights proved to be of 15 kDa for both lectins. Native molecular masses were of 30 and 32 kDa, for the ovary and the blastula lectin, respectively. Both lectins showed to be homodimers with non-covalently bound subunits. Isoelectrical points were 4,O and 4,7, for the ovary and the blastula lectin, respectively. Both lectins showed very strong immunological cross-reactivity, which suggests primary sequence homologies. Immunohistochemical studies demonstrated the localization of the embryonic lectin in the fertilization envelope, yolk platelets and celullar surfaces. Biological activities such as the block of the polispenny or adhesive-antiadhesive effects were suggested for these S-type lectins. Peptide or cDNA-sequencing information would be needed to classified them as galectins, and to determine the degree of homology between both.
Registro:
Título : Detección, cuantificación y caracterización de Lectinas ß-Galactosídicas de Bufo arenarum     =    Detection, quantification and characterization of ß-Galactoside-binding lectins from Bufo arenarum
Autor : Elola, María Teresa
Director : Fink de Cabutti, Nilda Ester
Año : 1995
Editor : Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires
Filiación : Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales
Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Hematología
Grado obtenido : Doctor en Ciencias Biológicas
Ubicación : Preservación - http://digital.bl.fcen.uba.ar/gsdl-282/cgi-bin/library.cgi?a=d&c=tesis&d=Tesis_2816_Elola
Idioma : Español
Area Temática : Biología / Biología Molecular y Celular
Biología / Embriología Animal
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