Artículo

Oviedo-Rouco, S.; Castro, M.A.; Alvarez-Paggi, D.; Spedalieri, C.; Tortora, V.; Tomasina, F.; Radi, R.; Murgida, D.H. "The alkaline transition of cytochrome c revisited: Effects of electrostatic interactions and tyrosine nitration on the reaction dynamics" (2019) Archives of Biochemistry and Biophysics. 665:96-106
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Abstract:

Here we investigated the effect of electrostatic interactions and of protein tyrosine nitration of mammalian cytochrome c on the dynamics of the so-called alkaline transition, a pH- and redox-triggered conformational change that implies replacement of the axial ligand Met80 by a Lys residue. Using a combination of electrochemical, time-resolved SERR spectroelectrochemical experiments and molecular dynamics simulations we showed that in all cases the reaction can be described in terms of a two steps minimal reaction mechanism consisting of deprotonation of a triggering group followed by ligand exchange. The pK a alk values of the transition are strongly modulated by these perturbations, with a drastic downshift upon nitration and an important upshift upon establishing electrostatic interactions with a negatively charged model surface. The value of pK a alk is determined by the interplay between the acidity of a triggering group and the kinetic constants for the forward and backward ligand exchange processes. Nitration of Tyr74 results in a change of the triggering group from Lys73 in WT Cyt to Tyr74 in the nitrated protein, which dominates the pK a alk downshift towards physiological values. Electrostatic interactions, on the other hand, result in strong acceleration of the backward ligand exchange reaction, which dominates the pK a alk upshift. The different physicochemical conditions found here to influence pK a alk are expected to vary depending on cellular conditions and subcellular localization of the protein, thus determining the existence of alternative conformations of Cyt in vivo. © 2019 Elsevier Inc.

Registro:

Documento: Artículo
Título:The alkaline transition of cytochrome c revisited: Effects of electrostatic interactions and tyrosine nitration on the reaction dynamics
Autor:Oviedo-Rouco, S.; Castro, M.A.; Alvarez-Paggi, D.; Spedalieri, C.; Tortora, V.; Tomasina, F.; Radi, R.; Murgida, D.H.
Filiación:Departamento de Química Inorgánica, Analítica y Química Física and INQUIMAE (CONICET-UBA), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pab. 2, piso 1, Buenos Aires, C1428EHA, Argentina
Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Av. Gral. Flores 2125, Montevideo, 11800, Uruguay
Palabras clave:Alkaline transition; Cytochrome c; Protein electron transfer; Protein nitration; Protein spectroelectrochemistry; Time-resolved SERR; cytochrome c; ligand; tyrosine; alkalinity; Article; cellular distribution; chemical reaction kinetics; conformational transition; cyclic potentiometry; deprotonation; electrochemical analysis; model; molecular dynamics; nitration; oxidation reduction reaction; pH; pKa; priority journal; protein conformation; Raman spectrometry; reaction analysis; static electricity; surface property
Año:2019
Volumen:665
Página de inicio:96
Página de fin:106
DOI: http://dx.doi.org/10.1016/j.abb.2019.02.016
Título revista:Archives of Biochemistry and Biophysics
Título revista abreviado:Arch. Biochem. Biophys.
ISSN:00039861
CODEN:ABBIA
CAS:cytochrome c, 9007-43-6, 9064-84-0; tyrosine, 16870-43-2, 55520-40-6, 60-18-4
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_00039861_v665_n_p96_OviedoRouco

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Citas:

---------- APA ----------
Oviedo-Rouco, S., Castro, M.A., Alvarez-Paggi, D., Spedalieri, C., Tortora, V., Tomasina, F., Radi, R.,..., Murgida, D.H. (2019) . The alkaline transition of cytochrome c revisited: Effects of electrostatic interactions and tyrosine nitration on the reaction dynamics. Archives of Biochemistry and Biophysics, 665, 96-106.
http://dx.doi.org/10.1016/j.abb.2019.02.016
---------- CHICAGO ----------
Oviedo-Rouco, S., Castro, M.A., Alvarez-Paggi, D., Spedalieri, C., Tortora, V., Tomasina, F., et al. "The alkaline transition of cytochrome c revisited: Effects of electrostatic interactions and tyrosine nitration on the reaction dynamics" . Archives of Biochemistry and Biophysics 665 (2019) : 96-106.
http://dx.doi.org/10.1016/j.abb.2019.02.016
---------- MLA ----------
Oviedo-Rouco, S., Castro, M.A., Alvarez-Paggi, D., Spedalieri, C., Tortora, V., Tomasina, F., et al. "The alkaline transition of cytochrome c revisited: Effects of electrostatic interactions and tyrosine nitration on the reaction dynamics" . Archives of Biochemistry and Biophysics, vol. 665, 2019, pp. 96-106.
http://dx.doi.org/10.1016/j.abb.2019.02.016
---------- VANCOUVER ----------
Oviedo-Rouco, S., Castro, M.A., Alvarez-Paggi, D., Spedalieri, C., Tortora, V., Tomasina, F., et al. The alkaline transition of cytochrome c revisited: Effects of electrostatic interactions and tyrosine nitration on the reaction dynamics. Arch. Biochem. Biophys. 2019;665:96-106.
http://dx.doi.org/10.1016/j.abb.2019.02.016