Artículo

Varela, M.L.; Ferreira, M.F.; Da Cuña, R.H.; Lo Nostro, F.L.; Genovese, G.; Meijide, F.J."Dynamics of ovarian maturation throughout the reproductive cycle of the neotropical cichlid fish Cichlasoma dimerus (Teleostei, Cichliformes)" (2017) Canadian Journal of Zoology. 95(7):485-498
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Abstract:

In this study, we analyzed gene expression profiles, plasma steroids concentrations, and gonadal morphology throughout the reproductive cycle of female Cichlasoma dimerus (Heckel, 1840), a monogamous cichlid fish exhibiting social hierarchies. Fish were analyzed at six phases encompassing their annual cycle, namely resting (during the nonreproductive period), prespawning, 30 h post spawning, 4 days post spawning, 10 days post spawning, and subordinate (during the reproductive period). The histological and histomorphometric analysis showed that C. dimerus exhibits asynchronous ovarian development. Similar to resting females, subordinate females showed low gonadosomatic index, reduced expression levels of vitellogenin (vtgAb), zona pellucida (zpB), gonadal aromatase (cyp19a1A), and low concentrations of plasma sex steroids, thus indicating that social intimidation by dominant conspecifics elicited reproductive arrest. In reproductively active females, a direct positive correlation between plasma estradiol, vtgAb expression, percentage of late vitellogenic oocytes, and gonadosomatic index was observed. These parameters were maximal at the prespawning phase, decreased at 30 h post spawning and 4 days post spawning, and then reached a peak at 10 days post spawning. Our results indicate that female C. dimerus become spawning capable after 10 days post spawning, coincidently with the shortest time interval between successive spawns recorded in captivity. © 2017, Canadian Science Publishing. All rights reserved.

Registro:

Documento: Artículo
Título:Dynamics of ovarian maturation throughout the reproductive cycle of the neotropical cichlid fish Cichlasoma dimerus (Teleostei, Cichliformes)
Autor:Varela, M.L.; Ferreira, M.F.; Da Cuña, R.H.; Lo Nostro, F.L.; Genovese, G.; Meijide, F.J.
Filiación:Laboratorio de Ecotoxicología Acuática, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires & Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad de Buenos Aires (UBA), Ciudad Autónoma de Buenos Aires, C1428EGA, Argentina
Palabras clave:Acará cichlid; Asynchronous ovarian development; Cichlasoma dimerus; Gene expression; Gonadal histology; Plasma steroids; Reproductive cycle; biochemical composition; blood; cichlid; correlation; gene expression; histology; maturation; monogamy; morphology; morphometry; Neotropical Region; physiology; reproductive cycle; spawning; steroid; Cichlasoma; Cichlidae; Teleostei
Año:2017
Volumen:95
Número:7
Página de inicio:485
Página de fin:498
DOI: http://dx.doi.org/10.1139/cjz-2016-0198
Handle:http://hdl.handle.net/20.500.12110/paper_00084301_v95_n7_p485_Varela
Título revista:Canadian Journal of Zoology
Título revista abreviado:Can. J. Zool.
ISSN:00084301
CODEN:CJZOA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084301_v95_n7_p485_Varela

Referencias:

  • Alonso, F., Cánepa, M., Guimarães Moreira, R., Pandolfi, M., Social and reproductive physiology and behavior of the Neotropical cichlid fish Cichlasoma dimerus under laboratory conditions (2011) Neotrop. Ichthyol, 9 (3), pp. 559-570
  • Babin, P.J., Carnevali, O., Lubzens, E., Schneider, W.J., Molecular aspects of oocyte vitellogenesis in fish (2007) The Fish Oocyte. from Basic Studies to Biotechnological Applications, pp. 39-76. , Edited by P.J. Babin, J. Cerda, and E. Lubzens. Springer, Dordrecht, the Netherlands
  • Birba, A., Ramallo, M.R., Lo Nostro, F., Guimarães Moreira, R., Pandolfi, M., Reproductive and parental care physiology of Cichlasoma dimerus males. Gen. Comp (2015) Endocrinol, 221, pp. 193-200. , PMID: 25688482
  • Böhne, A., Heule, C., Boileau, N., Salzburger, W., Expression and sequence evolution of aromatase cyp19a1 and other sexual development genes in east African cichlid fishes (2013) Mol. Biol. Evol, 30 (10), pp. 2268-2285. , PMID:23883521
  • Brodeur, J.C., Woodburn, K.B., Klecka, G.M., Potentiation of the vitellogenic response to 17_-ethinylestradiol by cortisol in the fathead minnow Pimephales promelas (2005) Environ. Toxicol. Chem, 24 (5), pp. 1125-1132. , PMID:16110990
  • (2005) Guidelines on the Care and the Use of Fish in Research, Teaching and Testing, , http://www.ccac.ca/Documents/Standards/Guidelines/Fish.pdf
  • Celius, T., Walther, B.T., Oogenesis in Atlantic salmon (Salmo salar L.) occurs by zonagenesis preceding vitellogenesis in vivo and in vitro (1998) J. Endocrinol, 158 (2), pp. 259-266. , PMID:9771470
  • Cerdá-Reverter, J.M., Canosa, L.F., Chapter 1: Neuroendocrine systems of the fish brain (2009) Fish Neuroendocrinol, 28 (9), pp. 3-74
  • Chang, X.T., Kobayashi, T., Kajiura, H., Nakamura, M., Nagahama, Y., Isolation and characterization of the cDNA encoding the tilapia (Oreochromis niloticus) cytochrome P450 aromatase (P450arom): Changes in P450arom mRNA, protein and enzyme activity in ovarian follicles during oogenesis (1997) J. Mol. Endocrinol, 18 (1), pp. 57-66. , PMID:9061607
  • Chang, X., Kobayashi, T., Senthilkumaran, B., Kobayashi-Kajura, H., Sudhakumari, C.C., Nagahama, Y., Two types of aromatase with different encoding genes, tissue distribution and developmental expression in Nile tilapia (Oreochromis niloticus) (2005) Gen. Comp. Endocrinol, 141 (2), pp. 101-115. , PMID:15748711
  • Connolly, M.H., Dutkosky, R.M., Heah, T.P., Sayler, G.S., Henry, T.B., Temporal dynamics of oocyte growth and vitellogenin gene expression in zebrafish (Danio rerio) (2014) Zebrafish, 11 (2), pp. 107-114. , PMID:24611554
  • Corriero, A., Acone, F., Desantis, S., Zubani, D., Deflorio, M., Ventriglia, G., Bridges, C.R., De Metrio, G., Histological and immunohistochemical investigation on ovarian development and plasma estradiol levels in the swordfish (Xiphias gladius L.) (2004) Eur. J. Histochem, 48 (4), pp. 413-422. , PMID:15718208
  • Costa Melo, R.M., Martins, Y.S., De Alencar Teixeira, E., Luz, R.K., Rizzo, E., Bazzoli, N., Morphological and quantitative evaluation of the ovarian recrudescence in Nile tilapia (Oreochromis niloticus) after spawning in captivity (2014) J. Morphol, 275, pp. 348-356. , PMID:24136463
  • Da Cuña, R.H., Pandolfi, M., Genovese, G., Piazza, Y., Ansaldo, M., Lo Nostro, F.L., Endocrine disruptive potential of endosulfan on the reproductive axis of Cichlasoma dimerus (Perciformes, Cichlidae) (2013) Aquat. Toxicol, 126, pp. 299-305. , PMID:23116938
  • Da Cuña, R.H., Rey Vázquez, G., Dorelle, L., Rodríguez, E.M., Guimarães Moreira, R., Lo Nostro, F.L., Mechanism of action of endosulfan as disruptor of gonadal steroidogenesis in the cichlid fish Cichlasoma dimerus (2016) Comp. Biochem. Physiol. Part C Toxicol. Pharmacol, 187, pp. 74-80
  • Di Yorio, M.P., Delgadin, T.H., Pérez Sirkin, D.I., Vissio, P.G., Growth hormone, luteinizing hormone, and follicle-stimulating hormone regulation by neuropeptide Y in both sexes of the cichlid fish, Cichlasoma dimerus (2015) Fish Physiol. Biochem, 41 (4), pp. 843-852. , PMID: 25869217
  • Eaton, R.C., Farley, R.D., Growth and the reduction of depensation of the zebrafish, Brachydanio rerio, reared in the laboratory (1974) Copeia, 1974 (1), pp. 204-209
  • Finn, R.N., Kolarevic, J., Kongshaug, H., Nilsen, F., Evolution and differential expression of a vertebrate vitellogenin gene cluster (2009) BMC Evol. Biol, 9, p. 2. , PMID:19123940
  • Gen, K., Yamaguchi, S., Okuzawa, K., Kumakura, N., Tanaka, H., Kagawa, H., Physiological roles of FSH and LH in red seabream, Pagrus major (2003) Fish Physiol. Biochem, 28 (1-4), pp. 77-80
  • Genovese, G., Da Cuña, R., Towle, D.W., Maggese, M.C., Lo Nostro, F., Early expression of zona pellucida proteins under octylphenol exposure in Cichlasoma dimerus (Perciformes, Cichlidae) (2011) Aquat. Toxicol, 101 (1), pp. 175-185. , PMID:21035200
  • Genovese, G., Regueira, M., Piazza, Y., Towle, D.W., Maggese, M.C., Lo Nostro, F., Time-course recovery of estrogen-responsive genes of a cichlid fish exposed to waterborne octylphenol (2012) Aquat. Toxicol, 114-115, pp. 1-13. , PMID:22410082
  • Genovese, G., Regueira, M., Da Cuña, R.H., Ferreira, M.F., Varela, M.L., Lo Nostro, F.L., Nonmonotonic response of vitellogenin and estrogen receptor gene expression after octylphenol exposure of Cichlasoma dimerus (Perciformes, Cichlidae) (2014) Aquat. Toxicol, 156, pp. 30-40. , PMID:25146234
  • Gohin, M., Bodinier, P., Fostier, A., Chesnel, F., Bobe, J., Aromatase is expressed and active in the rainbow trout oocyte during final oocyte maturation (2011) Mol. Reprod. Dev, 78 (7), pp. 510-518. , PMID:21656871
  • Gomez, J.M., Weil, C., Ollitrault, M., Le Bail, P.Y., Breton, B., Le Gac, F., Growth hormone (GH) and gonadotropin subunit gene expression and pituitary and plasma changes during spermatogenesis and oogenesis in rainbow trout (Oncorhynchus mykiss) (1999) Gen. Comp. Endocrinol, 113 (3), pp. 413-428. , PMID:10068502
  • Goudet, G., Mugnier, S., Callebaut, I., Monget, P., Phylogenetic analysis and identification of pseudogenes reveal a progressive loss of zona pellucida genes during evolution of vertebrates (2008) Biol. Reprod, 78 (5), pp. 796-806. , PMID:18046012
  • Grier, H.J., Uribe Aranzabal, M.C., Patino, R., The ovary, folliculogenesis and oogenesis in teleosts (2009) Reproductive Biology and Phylogeny of Fishes (Agnathans and Bony Fishes): Phylogeny, Reproductive System, Viviparity, Spermatozoa, pp. 25-84. , Edited by B.G.M. Jamieson. Science Publishers, Enfield, N.H
  • Hassin, S., Claire, M., Holland, H., Zohar, Y., Ontogeny of folliclestimulating hormone and luteinizing hormone gene expression during pubertal development in the female striped bass, Morone saxatilis (Teleostei) (1999) Biol. Reprod, 61 (6), pp. 1608-1615. , PMID:10570010
  • Hiramatsu, N., Todo, T., Sullivan, C.V., Schilling, J., Reading, B.J., Matsubara, T., Ryu, Y.W., Hara, A., Ovarian yolk formation in fishes: Molecular mechanisms underlying formation of lipid droplets and vitellogenin-derived yolk proteins (2015) Gen. Comp. Endocrinol, 221, pp. 9-15. , PMID:25660470
  • Huffman, L.S., O’Connell, L.A., Hofmann, H.A., Aromatase regulates aggression in the African cichlid fish Astatotilapia burtoni (2013) Physiol. Behav, 112-113, pp. 77-83. , PMID:23438371
  • Hyllner, S.J., Silversand, C., Haux, C., Formation of the vitelline envelope precedes the active uptake of vitellogenin during oocyte development in the rainbow trout, Oncorhynchus mykiss (1994) Mol. Reprod. Dev, 39 (2), pp. 166-175. , PMID:7826618
  • Kobayashi, Y., Kobayashi, T., Nakamura, M., Sunobe, T., Morrey, C.E., Suzuki, N., Nagahama, Y., Characterization of two types of cytochrome P450 aromatase in the serial-sex changing gobiid fish, Trimma okinawae (2004) Zool. Sci, 21 (4), pp. 417-425. , PMID:15118229
  • Kudo, N., Miura, T., Miura, C., Yamauchi, K., Expression and localization of eel testicular ZP-homologues in female Japanese eels (Anguilla japonica) (2000) Zool. Sci, 17 (9), pp. 1297-1302
  • Kumar, R.S., Ijiri, S., Trant, J.M., Changes in the expression of genes encoding steroidogenic enzymes in the channel catfish (Ictalurus punctatus) ovary throughout a reproductive cycle (2000) Biol. Reprod, 63 (6), pp. 1676-1682. , PMID:11090435
  • Le Menn, F., Cerdà, J., Babin, P.J., Ultrastructural aspects of the ontogeny and differentiation of ray-finned fish ovarian follicles (2007) The Fish Oocyte. from Basic Studies to Biotechnological Applications, pp. 1-37. , Edited by P.J. Babin, J. Cerda, and E. Lubzens. Springer, Dordrecht, the Netherland
  • Levavi-Sivan, B., Bogerd, J., Mañanós, E.L., Gómez, A., Lareyre, J.J., Perspectives on fish gonadotropins and their receptors (2010) Gen. Comp. Endocrinol, 165 (3), pp. 412-437. , PMID:19686749
  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem, 193 (1), pp. 265-275. , PMID:14907713
  • Lubzens, E., Young, G., Bobe, J., Cerdà, J., Oogenesis in teleosts: How fish eggs are formed (2010) Gen. Comp. Endocrinol, 165 (3), pp. 367-389. , PMID:19505465
  • Meijide, F.J., Guerrero, G.A., Embryonic and larval development of a substrate-brooding cichlid Cichlasoma dimerus (Heckel, 1840) under laboratory conditions (2000) J. Zool. (Lond.), 252 (4), pp. 481-493
  • Meijide, F.J., Rey Vázquez, G., Grier, H.J., Lo Nostro, F.L., Guerrero, G.A., Development of the germinal epithelium and early folliculogenesis during ovarian morphogenesis in the cichlid fish Cichlasoma dimerus (Teleostei, Perciformes) (2016) Acta Zool, 97, pp. 18-33
  • Mileva, V.R., Fitzpatrick, J.L., Marsh-Rollo, S., Gilmour, K.M., Wood, C.M., Balshine, S., The stress response of the highly social African cichlid Neolamprologus pulcher (2009) Physiol. Biochem. Zool, 82 (6), pp. 720-729. , PMID:19807269
  • Modig, C., Modesto, T., Canario, A., Cerdà, J., Von Hofsten, J., Olsson, P.-E., Molecular characterization and expression pattern of zona pellucida proteins in gilthead seabream (Sparus aurata) (2006) Biol. Reprod, 75 (5), pp. 717-725. , PMID:16855211
  • Modig, C., Westerlund, L., Olsson, P.E., Oocyte zona pellucida proteins (2007) The Fish Oocyte. from Basic Studies to Biotechnological Applications, pp. 113-139. , Edited by P.J. Babin, J. Cerda, and E. Lubzens. Springer, Dordrecht, the Netherlands
  • Moncaut, N., Lo Nostro, F., Maggese, M.C., Vitellogenin detection in surface mucus of the South American cichlid fish Cichlasoma dimerus (Heckel, 1840) induced by estradiol-17. Effects on liver and gonads (2003) Aquat. Toxicol, 63 (2), pp. 127-137. , PMID:12657488
  • Morandini, L., Honji, R.M., Ramallo, M.R., Guimarães Moreira, R., Pandolfi, M., The interrenal gland in males of the cichlid fish Cichlasoma dimerus: Relationship with stress and the establishment of social hierarchies (2014) Gen. Comp. Endocrinol, 195, pp. 88-98. , PMID: 24184109
  • Murua, H., Saborido-Rey, F., Female reproductive strategies of marine fish species of the North Atlantic (2003) J. Northw. Atl. Fish. Sci, 33, pp. 23-31
  • Nakamura, I., Kusakabe, M., Young, G., Regulation of steriodogenic enzyme mRNAs in rainbow trout (Oncorhynchus mykiss) ovarian follicles in vitro (2003) Fish Physiol. Biochem, 28 (1-4), pp. 355-356
  • Nasiadka, A., Clark, M.D., Zebrafish breeding in the laboratory environment (2012) ILAR J, 53 (2), pp. 161-168. , PMID:23382347
  • Nelson, E.R., Habibi, H.R., Estrogen receptor function and regulation in fish and other vertebrates (2013) Gen. Comp. Endocrinol, 192, pp. 15-24. , PMID:23583769
  • Pandolfi, M., Canepa, M.M., Meijide, F.J., Alonso, F., Vazquez, G.R., Maggese, M.C., Vissio, P.G., Studies on the reproductive and developmental biology of Cichlasoma dimerus (Percifomes, Cichlidae) (2009) Biocell, 33 (1), pp. 1-18. , PMID:19499881
  • Pereira, T.S.B., Guimarães Moreira, R., Batlouni, S.R., Dynamics of ovarian maturation during the reproductive cycle of Metynnis maculatus, a reservoir invasive fish species (Teleostei: Characiformes) (2013) Neotrop. Ichthyol, 11 (4), pp. 821-830
  • Piazza, Y., Pandolfi, M., Da Cuña, R., Genovese, G., Lo Nostro, F., Endosulfan affects GnRH cells in sexually differentiated juveniles of the perciform Cichlasoma dimerus (2015) Ecotoxicol. Environ. Saf, 116, pp. 150-159. , PMID:25800987
  • Polzonetti-Magni, A.M., Mosconi, G., Soverchia, L., Kikuyama, S., Carnevali, O., Multihormonal control of vitellogeneis in lower vertebrates (2004) Int. Rev. Cytol, 239, pp. 1-46. , PMID: 15464851
  • Ramallo, M.R., Grober, M., Cánepa, M.M., Morandini, L., Pandolfi, M., Arginine-vasotocin expression and participation in reproduction and social behavior in males of the cichlid fish Cichlasoma dimerus (2012) Gen. Comp. Endocrinol, 179 (2), pp. 221-231. , PMID:22940647
  • Ramallo, M.R., Morandini, L., Alonso, F., Birba, A., Tubert, C., Fiszbein, A., Pandolfi, M., The endocrine regulation of cichlids social and reproductive behavior through the eyes of the chanchita, Cichlasoma dimerus (Percomorpha; Cichlidae) (2014) J. Physiol, 108 (2), pp. 194-202
  • Ramallo, M.R., Birba, A., Honji, R.M., Morandini, L., Guimarães Moreira, R., Somoza, G.M., Pandolfi, M., A multidisciplinary study on social status and the relationship between inter-individual variation in hormone levels and agonistic behavior in a Neotropical cichlid fish (2015) Horm. Behav, 69, pp. 139-151. , PMID:25647157
  • Rey Vázquez, G., Meijide, F.J., Da Cuña, R.H., Lo Nostro, F.L., Piazza, Y.G., Babay, P.A., Trudeau, V.L., Guerrero, G.A., Exposure to waterborne 4-tert-octylphenol induces vitellogenin synthesis and disrupts testis morphology in the South American freshwater fish Cichlasoma dimerus (Teleostei, Perciformes) (2009) Comp. Biochem. Physiol. Part C Toxicol. Pharmacol, 150 (2), pp. 298-306
  • Rey Vázquez, G., Da Cuña, R.H., Meijide, F.J., Guerrero, G.A., Spermatogenesis and changes in testicular structure during the reproductive cycle in Cichlasoma dimerus (Teleostei, Perciformes) (2012) Acta Zool, 93 (3), pp. 338-350
  • Rocha, M.J., Reis-Henriques, M.A., Plasma and urine levels of C18, C19 and C21 steroids in an asynchronous fish, the tilapia Oreochromis mossambicus (Teleostei, Cichlidae) (1996) Comp. Biochem. Physiol. Part C Toxicol. Pharmacol, 115 (3), pp. 257-264
  • Roy Moulik, S., Pal, P., Majumder, S., Mallick, B., Gupta, S., Guha, P., Roy, S., Mukherjee, D., Gonadotropin and sf-1 regulation of cyp19a1a gene and aromatase activity during oocyte development in the rohu, L. Rohita (2016) Comp. Biochem. Physiol. Part a Mol. Integr. Physiol, 196, pp. 1-10
  • Schulz, R.W., Zandbergen, M.A., Peute, J., Bogerd, J., Van Dijk, W., Goos, H.J., Pituitary gonadotrophs are strongly activated at the beginning of spermatogenesis in African catfish, Clarias gariepinus (1997) Biol. Reprod, 57 (1), pp. 139-147. , PMID:9209092
  • Sohn, Y.C., Yoshiura, Y., Kobayashi, M., Aida, K., Seasonal changes in mRNA levels of gonadotropin and thyrotropin subunits in the goldfish, Carassius auratus (1999) Gen. Comp. Endocrinol, 113 (3), pp. 436-444. , PMID:10068504
  • Spargo, S., Hope, R., Evolution and nomenclature of the zona pellucida gene family (2003) Biol. Reprod, 68 (2), pp. 358-362. , PMID:12533396
  • Specker, J.L., Kishida, M., Mouthbrooding in the black-chinned tilapia, Sarotherodon melanotheron (Pisces: Cichlidae): The presence of eggs reduces androgen and estradiol levels during paternal and maternal parental behavior (2000) Horm. Behav, 38 (1), pp. 44-51. , PMID:10924286
  • Spence, R., Smith, C., Male territoriality mediates density and sex ratio effects on oviposition in the zebrafish, Danio rerio (2005) Anim. Behav, 69 (6), pp. 1317-1323
  • Spence, R., Ashton, R., Smith, C., Oviposition decisions are mediated by spawning site quality in wild and domesticated zebrafish, Danio rerio (2007) Behaviour, 144 (8), pp. 953-966
  • Tanaka, M., Telecky, T.M., Fukada, S., Adachi, S., Chen, S., Nagahama, Y., Cloning and sequence analysis of the cDNA encoding P-450 aromatase (P450arom) from a rainbow trout (Oncorhynchus mykiss) ovary; relationship between the amount of P450arom mRNA and the production of oestradiol- 17 in the ovary (1992) J. Mol. Endocrinol, 8 (1), pp. 53-61. , PMID:1543534
  • Taves, M.D., Desjardins, J.K., Mishra, S., Balshine, S., Androgens and dominance: Sex-specific patterns in a highly social fish (Neolamprologus pulcher) (2009) Gen. Comp. Endocrinol, 161 (2), pp. 202-207. , PMID:19174165
  • Tubert, C., Lo Nostro, F., Villafañe, V., Pandolfi, M., Aggressive behavior and reproductive physiology in females of the social cichlid fish Cichlasoma dimerus. Physiol (2012) Behav, 106 (2), pp. 193-200. , PMID:22342192
  • Tyler, C.R., Sumpter, J.P., Oocyte growth and development in teleosts (1996) Rev. Fish Biol. Fish, 6 (3), pp. 287-318
  • Van Der Kraak, G., The GnRH system and the neuroendocrine regulation of reproduction (2009) Fish Physiology. Vol. 28. Fish Neuroendocrinology, pp. 115-149. , Edited by N.J. Bernier, G. Van Der Kraak, A.P. Farrell, and C.J. Brauner. Academic Press, Burlington, Mass
  • Wallace, R.A., Selman, K., Oogenesis in Fundulus heteroclitus II. The transition from vitellogenesis into maturation (1980) Gen. Comp. Endocrinol, 42 (3), pp. 345-354. , PMID:7439674
  • Wallace, R.A., Selman, K., Cellular and dynamic aspects of oocyte growth in teleosts (1981) Am. Zool, 21 (2), pp. 325-343
  • Yang, C.G., Wang, X.L., Tian, J., Liu, W., Wu, F., Jiang, M., Wen, H., Evaluation of reference genes for quantitative real-time RT–PCR analysis of gene expression in Nile tilapia (Oreochromis niloticus) (2013) Gene, 527 (1), pp. 183-192. , PMID:23792389
  • Yost, E.E., Lee Pow, C., Hawkins, M.B., Kullman, S.W., Bridging the gap from screening assays to estrogenic effects in fish: Potential roles of multiple estrogen receptor subtypes (2014) Environ. Sci. Technol, 48 (9), pp. 5211-5219. , PMID:24422420
  • Zohar, Y., Muñoz-Cueto, J.A., Elizur, A., Kah, O., Neuroendocrinology of reproduction in teleost fish (2010) Gen. Comp. Endocrinol, 165 (3), pp. 438-455. , PMID:19393655

Citas:

---------- APA ----------
Varela, M.L., Ferreira, M.F., Da Cuña, R.H., Lo Nostro, F.L., Genovese, G. & Meijide, F.J. (2017) . Dynamics of ovarian maturation throughout the reproductive cycle of the neotropical cichlid fish Cichlasoma dimerus (Teleostei, Cichliformes). Canadian Journal of Zoology, 95(7), 485-498.
http://dx.doi.org/10.1139/cjz-2016-0198
---------- CHICAGO ----------
Varela, M.L., Ferreira, M.F., Da Cuña, R.H., Lo Nostro, F.L., Genovese, G., Meijide, F.J. "Dynamics of ovarian maturation throughout the reproductive cycle of the neotropical cichlid fish Cichlasoma dimerus (Teleostei, Cichliformes)" . Canadian Journal of Zoology 95, no. 7 (2017) : 485-498.
http://dx.doi.org/10.1139/cjz-2016-0198
---------- MLA ----------
Varela, M.L., Ferreira, M.F., Da Cuña, R.H., Lo Nostro, F.L., Genovese, G., Meijide, F.J. "Dynamics of ovarian maturation throughout the reproductive cycle of the neotropical cichlid fish Cichlasoma dimerus (Teleostei, Cichliformes)" . Canadian Journal of Zoology, vol. 95, no. 7, 2017, pp. 485-498.
http://dx.doi.org/10.1139/cjz-2016-0198
---------- VANCOUVER ----------
Varela, M.L., Ferreira, M.F., Da Cuña, R.H., Lo Nostro, F.L., Genovese, G., Meijide, F.J. Dynamics of ovarian maturation throughout the reproductive cycle of the neotropical cichlid fish Cichlasoma dimerus (Teleostei, Cichliformes). Can. J. Zool. 2017;95(7):485-498.
http://dx.doi.org/10.1139/cjz-2016-0198