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Abstract:

This study evaluated by immunohistochemical and Western blot methods, the distribution of two distinct gonadotropin-releasing hormones (GnRHs), corresponding to catfish GnRH (cfGnRH or GnRH1) and chicken-II GnRH (cGnRH-II or GnRH2), in Steindachneridion parahybae females in captivity, focusing these analyses on the reproductive cycle by semi-quantification of optical density (OD). Further, we found that the GnRH neuronal systems co-localized with their respective GnRH-associated peptides (GAPs). A group of neurons immunoreactive (ir) to GnRH1 were identified along the ventral region of the olfactory bulb (vOB) in the telencephalon (vTel) and in the main areas of the diencephalon (especially the medial basal hypothalamus, HBM), including fibers extending into the pituitary gland. In contrast, GnRH2 neurons were confined to the midbrain tegmentum, close to the ventricular surface, without projections to the pituitary gland. Moreover, a cfGAP (GnRH1)-specific band (9 kDa) was identified in the brain and pituitary gland, while a cGAP-II (GnRH2)-specific band (26 kDa) was observed only in the brain extract. During the reproductive cycle, GnRH1-ir presented greater OD values at the vitellogenic and regression stages than at the previtellogenic stage and after artificially induced to spawn. Larger GnRH2-ir neurons were observed during the reproductive cycle, but a higher OD was identified only in the regression stage compared with the other maturation stages. Finally, GnRH1 axons were found to be directed towards the pituitary, and this GnRH type, which is probably the hypophysiotropic form, can contribute to the reproductive dysfunction that occurs in S. parahybae females in captivity, whereas GnRH2 may act as a neuromodulator and/or neurotransmitter. © 2018 Elsevier Inc.

Registro:

Documento: Artículo
Título:Characterization of the gonadotropin-releasing hormone system in the Neotropical teleost, Steindachneridion parahybae during the annual reproductive cycle in captivity
Autor:Honji, R.M.; Caneppele, D.; Pandolfi, M.; Lo Nostro, F.L.; Moreira, R.G.
Filiación:Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, Travessa 14, 321, São Paulo, SP 05508-090, Brazil
Centro de Aquicultura, Universidade Estadual Paulista (UNESP), Campus de Jaboticabal, Via de Acesso Prof. Paulo Donato Castellane, S/N, 14884-900, Jaboticabal, São Paulo, Brazil
Companhia Energética de São Paulo (CESP), Unidade de Hidrobiologia e Aquicultura, Rodovia dos Tamoios, km 38. 12260-000, Brazil
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires & IBBEA, CONICET-UBA, Ciudad Universitaria (C1428EHA), Buenos Aires, Argentina
Palabras clave:GnRH; Hypothalamus; Pituitary gland; Reproduction dysfunction; Seasonal reproduction
Año:2019
Volumen:273
Página de inicio:73
Página de fin:85
DOI: http://dx.doi.org/10.1016/j.ygcen.2018.05.007
Título revista:General and Comparative Endocrinology
Título revista abreviado:Gen. Comp. Endocrinol.
ISSN:00166480
CODEN:GCENA
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_00166480_v273_n_p73_Honji

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Citas:

---------- APA ----------
Honji, R.M., Caneppele, D., Pandolfi, M., Lo Nostro, F.L. & Moreira, R.G. (2019) . Characterization of the gonadotropin-releasing hormone system in the Neotropical teleost, Steindachneridion parahybae during the annual reproductive cycle in captivity. General and Comparative Endocrinology, 273, 73-85.
http://dx.doi.org/10.1016/j.ygcen.2018.05.007
---------- CHICAGO ----------
Honji, R.M., Caneppele, D., Pandolfi, M., Lo Nostro, F.L., Moreira, R.G. "Characterization of the gonadotropin-releasing hormone system in the Neotropical teleost, Steindachneridion parahybae during the annual reproductive cycle in captivity" . General and Comparative Endocrinology 273 (2019) : 73-85.
http://dx.doi.org/10.1016/j.ygcen.2018.05.007
---------- MLA ----------
Honji, R.M., Caneppele, D., Pandolfi, M., Lo Nostro, F.L., Moreira, R.G. "Characterization of the gonadotropin-releasing hormone system in the Neotropical teleost, Steindachneridion parahybae during the annual reproductive cycle in captivity" . General and Comparative Endocrinology, vol. 273, 2019, pp. 73-85.
http://dx.doi.org/10.1016/j.ygcen.2018.05.007
---------- VANCOUVER ----------
Honji, R.M., Caneppele, D., Pandolfi, M., Lo Nostro, F.L., Moreira, R.G. Characterization of the gonadotropin-releasing hormone system in the Neotropical teleost, Steindachneridion parahybae during the annual reproductive cycle in captivity. Gen. Comp. Endocrinol. 2019;273:73-85.
http://dx.doi.org/10.1016/j.ygcen.2018.05.007