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Abstract:

The honey bee is the most frequently used species in pollination services for diverse crops. In onion crops (Allium cepa), however, bees avoid visiting certain varieties, being attracted differently to male sterile (MS) and fertile (OP) lines. These differences might be based on the phenolic profiles of the cultivars’ nectars. To understand the relationship between nectar composition and pollinator attraction to different onion lines, we tested sensory and cognitive abilities and palatability in honey bees exposed to MS and OP onion nectars and sugar solutions mimicking them. We evaluated the proboscis extension response (PER) after antennal contact (unconditioned response) to MS or OP onion nectars, finding no statistical differences, which indicates similar gustatory perception for the two nectars. We also performed food uptake assays to test palatability of different artificial nectars, considering their flavonoids and potassium content. The presence of potassium decreased the palatability of the artificial nectars. Finally, we evaluated the bees’ cognitive abilities when the reward (unconditioned stimulus) offered during conditioning PER assays presents differences in composition. We found that potassium by itself impaired learning; however, such impairment was even higher when naringenin and quercetin were added in the unconditioned stimulus (MS nectar mimic). Interestingly, potassium together with luteolin (OP nectar mimic) improved learning. Our study demonstrates that the differences in the nectars’ flavonoid profiles combined with their high potassium content could explain the previously reported differences in attractiveness between onion lines, suggesting an important role of nectar compounds other than sugars for the attractiveness of flowers to pollinators. © 2019. Published by The Company of Biologists Ltd

Registro:

Documento: Artículo
Título:Appetitive behavior of the honey bee Apis mellifera in response to phenolic compounds naturally found in nectars
Autor:Hernández, I.G.; Palottini, F.; Macri, I.; Galmarini, C.R.; Farina, W.M.
Filiación:Instituto Nacional de Tecnologıá Agropecuaria (INTA), Estación Experimental Agropecuaria La Consulta, La Consulta, Mendoza, M5567, Argentina
Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Mendoza, M5502 JMA, Argentina
Consejo Nacional de Investigaciones Cientıficas y Técnicas (CONICET), Buenos Aires, Argentina
Laboratorio de Insectos Sociales, Departamento de Biodiversidad y Biologıá Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, FH55+63, Argentina
Instituto de Fisiologıá, Biologıá Molecular y Neurociencias (IFIBYNE), CONICET-Universidad de Buenos Aires, Buenos Aires, FH56+2C, Argentina
Instituto de Ingenierıá Rural, Centro de Investigación de Agroindustria (CIA), Instituto Nacional de Tecnologıá Agropecuaria (INTA), 98WW+CR, Castelar, Argentina
Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Mendoza, M5502 JMA, Argentina
Palabras clave:Flavonoid; Food uptake; Olfactory learning; Onion nectar; Pollination; Responsiveness
Año:2019
Volumen:222
Número:2
DOI: http://dx.doi.org/10.1242/jeb.189910
Título revista:Journal of Experimental Biology
Título revista abreviado:J. Exp. Biol.
ISSN:00220949
CODEN:JEBIA
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_00220949_v222_n2_p_Hernandez

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Citas:

---------- APA ----------
Hernández, I.G., Palottini, F., Macri, I., Galmarini, C.R. & Farina, W.M. (2019) . Appetitive behavior of the honey bee Apis mellifera in response to phenolic compounds naturally found in nectars. Journal of Experimental Biology, 222(2).
http://dx.doi.org/10.1242/jeb.189910
---------- CHICAGO ----------
Hernández, I.G., Palottini, F., Macri, I., Galmarini, C.R., Farina, W.M. "Appetitive behavior of the honey bee Apis mellifera in response to phenolic compounds naturally found in nectars" . Journal of Experimental Biology 222, no. 2 (2019).
http://dx.doi.org/10.1242/jeb.189910
---------- MLA ----------
Hernández, I.G., Palottini, F., Macri, I., Galmarini, C.R., Farina, W.M. "Appetitive behavior of the honey bee Apis mellifera in response to phenolic compounds naturally found in nectars" . Journal of Experimental Biology, vol. 222, no. 2, 2019.
http://dx.doi.org/10.1242/jeb.189910
---------- VANCOUVER ----------
Hernández, I.G., Palottini, F., Macri, I., Galmarini, C.R., Farina, W.M. Appetitive behavior of the honey bee Apis mellifera in response to phenolic compounds naturally found in nectars. J. Exp. Biol. 2019;222(2).
http://dx.doi.org/10.1242/jeb.189910