Artículo
Balzarotti, F.; Eilers, Y.; Gwosch, K.C.; Gynnå, A.H.; Westphal, V.; Stefani, F.D.; Elf, J.; Hell, S.W. "Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes" (2017) Science. 355(6325):606-612
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Abstract:
We introduce MINFLUX, a concept for localizing photon emitters in space. By probing the emitter with a local intensity minimum of excitation light, MINFLUX minimizes the fluorescence photons needed for high localization precision. In our experiments, 22 times fewer fluorescence photons are required as compared to popular centroid localization. In superresolution microscopy, MINFLUX attained ∼1-nanometer precision, resolving molecules only 6 nanometers apart. MINFLUX tracking of single fluorescent proteins increased the temporal resolution and the number of localizations per trace by a factor of 100, as demonstrated with diffusing 30S ribosomal subunits in living Escherichia coli. As conceptual limits have not been reached, we expect this localization modality to break new ground for observing the dynamics, distribution, and structure of macromolecules in living cells and beyond. © 2017, American Association for the Advancement of Science. All rights reserved.
Registro:
| Documento: | Artículo |
| Título: | Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes |
| Autor: | Balzarotti, F.; Eilers, Y.; Gwosch, K.C.; Gynnå, A.H.; Westphal, V.; Stefani, F.D.; Elf, J.; Hell, S.W. |
| Filiación: | Department of NanoBiophotonics, Max Planck Institute For Biophysical Chemistry, Göttingen, Germany Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina Departamento de Física, Facultad de Ciencias Exactas y Naturales, University of Buenos Aires, Buenos Aires, Argentina Department of Optical Nanoscopy, Max Planck Institute for Medical Research, Heidelberg, Germany Optical Nanoscopy Division, German Cancer Research Center (DKFZ), Heidelberg, Germany |
| Palabras clave: | fluorescent dye; protein; DNA; photoprotein; cell organelle; cells and cell components; coliform bacterium; equipment; fluorescence; image resolution; light intensity; precision; protein; Article; Escherichia coli; excitation; fluorescence; fluorescence microscopy; light; macromolecule; molecular dynamics; nanoimaging; photon; priority journal; ribosome subunit; simulation; chemistry; fluorescence imaging; nanotechnology; photon; procedures; single molecule imaging; small ribosomal subunit; Escherichia coli; DNA; Escherichia coli; Luminescent Proteins; Microscopy, Fluorescence; Nanotechnology; Optical Imaging; Photons; Ribosome Subunits, Small, Bacterial; Single Molecule Imaging |
| Año: | 2017 |
| Volumen: | 355 |
| Número: | 6325 |
| Página de inicio: | 606 |
| Página de fin: | 612 |
| DOI: | http://dx.doi.org/10.1126/science.aak9913 |
| Título revista: | Science |
| Título revista abreviado: | Sci. |
| ISSN: | 00368075 |
| CODEN: | SCIEA |
| CAS: | protein, 67254-75-5; DNA, 9007-49-2; DNA; Luminescent Proteins |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00368075_v355_n6325_p606_Balzarotti |
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Citas:
---------- APA ----------
Balzarotti, F., Eilers, Y., Gwosch, K.C., Gynnå, A.H., Westphal, V., Stefani, F.D., Elf, J.,..., Hell, S.W. (2017) . Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes. Science, 355(6325), 606-612.http://dx.doi.org/10.1126/science.aak9913
---------- CHICAGO ----------
Balzarotti, F., Eilers, Y., Gwosch, K.C., Gynnå, A.H., Westphal, V., Stefani, F.D., et al. "Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes" . Science 355, no. 6325 (2017) : 606-612.http://dx.doi.org/10.1126/science.aak9913
---------- MLA ----------
Balzarotti, F., Eilers, Y., Gwosch, K.C., Gynnå, A.H., Westphal, V., Stefani, F.D., et al. "Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes" . Science, vol. 355, no. 6325, 2017, pp. 606-612.http://dx.doi.org/10.1126/science.aak9913
---------- VANCOUVER ----------
Balzarotti, F., Eilers, Y., Gwosch, K.C., Gynnå, A.H., Westphal, V., Stefani, F.D., et al. Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes. Sci. 2017;355(6325):606-612.http://dx.doi.org/10.1126/science.aak9913
