Colombo, R.P.; Ibarra, J.G.; Bidondo, L.F.; Silvani, V.A.; Bompadre, M.J.; Pergola, M.; López, N.I.; Godeas, A.M. "Arbuscular mycorrhizal fungal association in genetically modified drought-tolerant corn" (2017) Journal of Environmental Quality. 46(1):227-231
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The commercial use of genetically modified (GM) plants has significantly increased worldwide. The interactions between GM plants and arbuscular mycorrhizal (AM) fungi are of considerable importance given the agricultural and ecological role of AM and the lack of knowledge regarding potential effects of droughttolerant GM corn (Zea mays L.) on AM fungal symbiosis. This work studied AM fungal colonization in five corn lines growing under two different irrigation regimes (30 and 100% of soil field capacity [SFC]). Four of the lines were GM corn, and two of these were drought tolerant. The experiment was conducted for 60 d in a growth chamber under constant irrigation, after which mycorrhization, corn biomass, and days to plant senescence (DTS) were evaluated. Arbuscular mycorrhizal fungal species of the order Diversisporales were predominant in the soil inocula. At the end of the experiment, all plants showed AM colonization. Mycorrhization was higher at 30% SFC than at 100% SFC. Within the same corn line, the AM fungi produced more vesicles in plant roots under drought stress. Among treatments, DTS varied significantly, and droughttolerant GM corn lines survived longer than the wild-type corn when maintained at 100% SFC. Corn biomass did not vary among treatments, and no correlations were found between DTS or biomass and mycorrhization. We conclude that overexpression of the Hahb-4 gene in corn plants under the experimental conditions of this study did not affect AM fungal infectivity and improved the tolerance of the corn to drought stress. © American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America. 5585 Guilford Rd., Madison, WI 53711 USA. All rights reserved.


Documento: Artículo
Título:Arbuscular mycorrhizal fungal association in genetically modified drought-tolerant corn
Autor:Colombo, R.P.; Ibarra, J.G.; Bidondo, L.F.; Silvani, V.A.; Bompadre, M.J.; Pergola, M.; López, N.I.; Godeas, A.M.
Filiación:Dep. de Biodiversidad y Biología Experimental, FCEN, Univ. de Buenos Aires, IBBEA-CONICET, Argentina
Dep. de Química Biológica, FCEN, Univ. de Buenos Aires, IQUIBICEN-CONICET, Argentina
Dep. de Ciencia y Tecnología, Univ. Nacional de Quilmes, Argentina
Palabras clave:Biomass; Drought; Ecology; Fungi; Irrigation; Arbuscular mycorrhizal; Arbuscular mycorrhizal fungi; Corn (Zea mays L.); Experimental conditions; Fungal colonization; Genetically modified; Irrigation regimes; Potential effects; Genetically modified plants; ethylene; transcription factor; Article; biomass; Diversisporales; drought stress; drought tolerance; environmental stress; fungal colonization; fungus; maize; nonhuman; phenotype; plant root; senescence; soil inoculation; symbiosis; transgenic plant; drought; genetics; maize; mycorrhiza; Biomass; Droughts; Mycorrhizae; Plant Roots; Plants, Genetically Modified; Symbiosis; Zea mays
Página de inicio:227
Página de fin:231
Título revista:Journal of Environmental Quality
Título revista abreviado:J. Environ. Qual.
CAS:ethylene, 74-85-1


  • Blaszkowski, J., (2003) Taxonomy of arbusculuar fungi, ,, West Pomeranian University of Technology, Szczecin (accessed 6 Oct. 2016)
  • Bompadre, M.J., Silvani, V.A., Fernandez Bidondo, L., Rios de Molina, M.C., Colombo, R.P., Pardo, A.G., Godeas, A.M., Arbuscular mycorrhizal fungi alleviate oxidative stress in pomegranate plants growing under different irrigation conditions (2014) Botany, 92, pp. 187-193
  • Bompadre, M.J., Fernandez Bidondo, L., Silvani, V.A., Colombo, R.P., Pergola, M., Pardo, A.G., Godeas, A.M., Combined effects of arbuscular mycorrhizal fungi and exogenous cytokinins on pomegranate (Punica granatum) under two contrasting water availability conditions (2015) Symbiosis, 65 (2), pp. 55-63
  • Bonfante, P., Genre, A., Mechanisms underlying beneficial plantsfungus interactions in mycorrhizal symbiosis (2010) Nat. Commun, 1, p. 48
  • Cabello, M.N., Hydrocarbon pollution: Its effect on native arbuscular mycorrhizal fungi (AMF) (1997) FEMS Microbiol. Ecol, 22, pp. 233-236
  • Castaldini, M., Turrini, A., Sbrana, C., Benedetti, A., Marchionni, M., Fabiani, A., Landi, S., Giovannetti, M., Impact of Bt corn on rhizospheric and soil eubacterial communities and on beneficial mycorrhizal symbiosis in experimental microcosms (2005) Appl. Environ. Microbiol, 71, pp. 6719-6729
  • Cuenca, G., (2015) Las micorrizas arbusculares: Aspectos teóricos y aplicados, , Ediciones IVIC, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela
  • De Martonne, E., Une nouvelle fonction climatologique: L'indice d'aridité (1926) Meteorologie, 2, pp. 449-458
  • Declerck, S., Strullu, D.G., Plenchette, C., In vitro mass-production of the arbuscular mycorrhizal fungus, Glomus versiforme, associated with Ri T-DNA transformed carrot roots (1996) Mycol. Res, 100 (10), pp. 1237-1242
  • Dumbrell, A.J., Ashton, P.D., Aziz, A., Feng, G., Nelson, M., Dytham, C., Fitter, A.H., Helgason, T., Distinct seasonal assemblage of arbuscular mycorrhizal fungi revealed by massively parallel pyrosequencing (2011) New Phytol, 190, pp. 794-804
  • Giovannetti, M., The ecological risks of transgenic plants (2003) Riv. Biol, 96, pp. 207-223
  • Hallauer, A.R., Lamkey, K.R., White, P.R., Registration of five inbred lines of maize: B102, B103, B104, B105, and B106 (1997) Crop Sci, 37, pp. 1405-1406
  • Hart, M.M., Reader, R.J., Taxonomic basis for variation in the colonization strategy or arbuscular mycorrhizal fungi (2002) New Phytol, 153, pp. 335-344
  • (2016),, Index fungorum (accessed 12 Oct. 2016); Liang, Z., Drijber, R.A., Lee, D.J., Dwiekat, I.M., Harris, S.D., Wedin, D.A., A DGGE-cloning method to characterize arbuscular mycorrhizal community structure in soil (2008) Soil Biol. Biochem, 40, pp. 956-966
  • Liu, W., Do genetically modified plants impact arbuscular mycorrhizal fungi? (2010) Ecotoxicology, 19, pp. 229-238
  • Liu, W.K., Du, L.F., Interactions between Bt transgenic crops and arbuscular mycorrhizal fungi: A new urgent soil ecology issue in agro-ecosystems (2008) Acta Agric. Scand. Sect. B, 58, pp. 187-192
  • Lumini, E., Orgiazzi, A., Borriello, R., Bonfante, P., Bianciotto, V., Disclosing arbuscular mycorrhizal fungal biodiversity in soil through a land-use gradient using a pyrosequencing approach (2010) Environ. Microbiol, 12, pp. 2165-2179
  • Manavella, P.A., Arce, A.L., Dezar, C.A., Bitton, F., Renou, J.P., Crespi, M., Chan, R.L., Cross-talk between ethylene and drought signaling pathways is mediated by the sunflower Hahb-4 transcription factor (2006) Plant J, 48, pp. 125-137
  • Manavella, P.A., Dezar, C.A., Bonaventure, G., Baldwin, I.T., Chan, R.L., HAHB4, a sunflower HD-Zip protein, integrates signals from the jasmonic acid and ethylene pathways during wounding and biotic stress responses (2008) Plant J, 56 (3), pp. 376-388
  • Morton, J., (2014) International culture collection of (vesicular) arbuscular mycorrhizal fungi, ,, West Virginia Univ., Morgantown (accessed 12 Oct. 2016)
  • Newhouse, A.E., Schrodt, F., Liang, H.Y., Maynard, C.A., Powell, W.A., Transgenic American elm shows reduced Dutch elm disease symptoms and normal mycorrhizal colonization (2007) Plant Cell Rep, 26, pp. 977-987
  • Parniske, M., Arbuscular mycorrhiza: The mother of plant root endosymbioses (2008) Nat. Rev. Microbiol, 6, pp. 763-775
  • Pearson, K., Mathematical contributions to the theory of evolution, III: Regression heredity and panmixia (1896) Philos. Trans. R. Soc. A, 187, pp. 253-318. , (reprinted in Karl Parson's Early Statistical Papers 1956:113-178, Cambridge Univ. Press)
  • Phillips, J.M., Hayman, D.S., Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection (1970) Trans. Br. Mycol. Soc, 55 (1), pp. 158-160
  • Plenchette, C., Morel, C., External phosphorus requirements of mycorrhizal and non-mycorrhizal barley and soybean plants (1996) Biol. Fertil. Soils, 21, pp. 303-308
  • Turrini, A., Sbrana, C., Nuti, M.P., Pietrangeli, B., Giovannetti, M., Development of a model system to assess the impact of genetically modified corn and eggplant on arbuscular mycorrhizal fungi (2005) Plant Soil, 266, pp. 69-75
  • Turrini, A., Sbrana, C., Giovanetti, M., Belowground environmental effects of transgenic crops: A soil microbial perspective (2015) Res. Microbiol, 166, pp. 121-131
  • Vierheiling, H., Alt, M., Lange, J., Gut-Rella, M., Wiemken, A., Boller, T., Colonization of transgenic tobacco constitutively expressing pathogenesisrelated proteins by vesicular-arbuscular mycorrhizal fungus Glomus mosseae (1995) Appl. Environ. Microbiol, 61, pp. 3031-3034
  • Waterer, D.R., Coltman, R.R., Response of mycorrhizal bell peppers to inoculation timing, phosphorus and water stress (1989) HortScience, 24, pp. 688-690


---------- APA ----------
Colombo, R.P., Ibarra, J.G., Bidondo, L.F., Silvani, V.A., Bompadre, M.J., Pergola, M., López, N.I.,..., Godeas, A.M. (2017) . Arbuscular mycorrhizal fungal association in genetically modified drought-tolerant corn. Journal of Environmental Quality, 46(1), 227-231.
---------- CHICAGO ----------
Colombo, R.P., Ibarra, J.G., Bidondo, L.F., Silvani, V.A., Bompadre, M.J., Pergola, M., et al. "Arbuscular mycorrhizal fungal association in genetically modified drought-tolerant corn" . Journal of Environmental Quality 46, no. 1 (2017) : 227-231.
---------- MLA ----------
Colombo, R.P., Ibarra, J.G., Bidondo, L.F., Silvani, V.A., Bompadre, M.J., Pergola, M., et al. "Arbuscular mycorrhizal fungal association in genetically modified drought-tolerant corn" . Journal of Environmental Quality, vol. 46, no. 1, 2017, pp. 227-231.
---------- VANCOUVER ----------
Colombo, R.P., Ibarra, J.G., Bidondo, L.F., Silvani, V.A., Bompadre, M.J., Pergola, M., et al. Arbuscular mycorrhizal fungal association in genetically modified drought-tolerant corn. J. Environ. Qual. 2017;46(1):227-231.