Abstract:
Water-soluble sulfated polysaccharides from the red seaweed Nemalion helminthoides: two xylomannan fractions (N3 and N4) and a mannan fraction (N6) were investigated to determine their in vitro and in vivo immunomodulatory activities. N3 and N4 induced in vitro proliferation of macrophages of the murine cell line RAW 264.7 and significantly stimulated the production of nitric oxide (NO) and cytokines (IL-6 and TNF-α) in the same cells, whereas this response was not observed with the mannan N6. The cytokine production was also stimulated by sulfated xylomannans in vivo in BALB/c mice inoculated intravenously with these polysaccharides. Remarkably, when mice were treated with N3 and N4 for 1. h before being infected with Herpes simplex virus type 2, they remained asymptomatic with no signs of disease. The in vitro and in vivo results suggest that sulfated xylomannans could be strong immunomodulators. © 2013 Elsevier B.V.
Registro:
Documento: |
Artículo
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Título: | In vitro and in vivo immunomodulatory activity of sulfated polysaccharides from red seaweed Nemalion helminthoides |
Autor: | Pérez-Recalde, M.; Matulewicz, M.C.; Pujol, C.A.; Carlucci, M.J. |
Filiación: | Departamento de Química Orgánica-CIHIDECAR (CONICET-UBA), Facultad de Ciencias. Exactas y Naturales, UBA, Pabellón 2, Ciudad Universitaria, 1428EGA Buenos Aires, Argentina Laboratorio de Virología, Departamento de Química Biológica-IQUIBICEN (CONICET-UBA), Facultad de Ciencias Exactas y Naturales UBA, Pabellón 2, Ciudad Universitaria, 1428EGA Buenos Aires, Argentina
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Palabras clave: | Immunomodulatory activity; Nemalion helminthoides; Sulfated xylomannans; immunomodulating agent; interleukin 6; mannan; mannan fraction; nitric oxide; polysaccharide sulfate; sulfated mannan; sulfated xylomannan; tumor necrosis factor alpha; unclassified drug; animal cell; animal experiment; animal model; article; cell proliferation; concentration response; controlled study; cytokine production; cytokine release; cytotoxicity; drug activity; herpes simplex; Herpes simplex virus 2; immunomodulation; in vitro study; in vivo study; infection prevention; macrophage; male; mouse; nonhuman; protein blood level; red alga; Human herpesvirus 2; Murinae; Mus; Nemalion helminthoides; Immunomodulatory activity; Nemalion helminthoides; Sulfated xylomannans; Animals; Cell Proliferation; Immunomodulation; Interleukin-6; Macrophages; Mice; Nitric Oxide; Oligosaccharides; Polysaccharides; Seaweed; Tumor Necrosis Factor-alpha |
Año: | 2014
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Volumen: | 63
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Página de inicio: | 38
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Página de fin: | 42
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DOI: |
http://dx.doi.org/10.1016/j.ijbiomac.2013.10.024 |
Título revista: | International Journal of Biological Macromolecules
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Título revista abreviado: | Int. J. Biol. Macromol.
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ISSN: | 01418130
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CODEN: | IJBMD
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CAS: | mannan, 51395-96-1, 9036-88-8; nitric oxide, 10102-43-9; polysaccharide sulfate, 79933-28-1
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01418130_v63_n_p38_PerezRecalde |
Referencias:
- Mourao, P.A.S., (2007) Braz. J. Med. Biol. Res., 40, pp. 5-17
- Nader, H.B., Lopes, C.C., Rocha, H.A.O., Santos, E.A., Dietrich, C.P., (2004) Curr. Pharm. Des., 10, pp. 951-966
- Usov, A., (2011) Adv. Carbohydr. Chem. Biochem., 65, pp. 115-217
- Na, S., Kim, W., Kim, S., Park, J., Lee, S., Kim, A., Park, Y., (2010) Int. Immunopharmacol., 10, pp. 364-370
- Schepetkin, I.A., Quinn, M.T., (2006) Int. Immunopharmacol., 6, pp. 317-333
- Wasser, S., (2002) Appl. Microbiol. Biotechnol., 60, pp. 258-274
- Thakur, M., Weng, A., Fuchs, H., Sharma, V., (2012) Carbohydr. Polym., 87, pp. 3-15
- Jiang, Z., Okimura, T., Yamaguchi, K., Oda, T., (2011) Nitric Oxide, 25, pp. 407-415
- Pugh, N., Ross, S.A., Elsohly, H.N., Pasco, D.S., (2001) Planta Med., 67, pp. 737-742
- Abdala-Díaz, R., Chabrillon, M., Cabello-Pasini, A., Gómez-Pinchetti, J., Figueroa, F., (2011) J. Appl. Phycol., 23, pp. 523-528
- Karnjanapratum, S., You, S., (2011) Int. J. Biol., 48, pp. 311-318
- Yermak, I.M., Barabanova, A.O., Aminin, D.L., Davydova, V.N., Sokolova, E.V., Soloveva, T.F., Kim, Y.H., Shin, K.S., (2011) Carbohydr. Polym., 87, pp. 713-720
- Pérez Recalde, M., Noseda, M., Pujol, C.A., Carlucci, M.J., Matulewicz, M.C., (2009) Phytochemistry, 70, pp. 1062-1068
- Pérez Recalde, M., Carlucci, M.J., Noseda, M., Matulewicz, M.C., (2011) Phytochemistry, 73, pp. 57-64
- Kolender, A.A., Pujol, C.A., Damonte, E.B., Matulewicz, M.C., Cerezo, A.S., (1997) Carbohydr. Res., 304, pp. 53-60
- Gamal-Eldeen, A.M., Amer, H., Helmy, W.A., Talaat, R.M., Ragab, H., (2007) Int. Immunopharmacol., 7, pp. 871-878
- Carlucci, M.J., Mateu, C.G., Artuso, M.C., Scolaro, L.A., (2012) The Complex World of Polysaccharides, , InTech, Croacia, D.N. Karunaratne (Ed.)
- Liu, C., Leung, M.Y., Koon, J.M.C., Zhu, L.F., Hiu, Y.Z., Yu, B., Fung, K.P., (2006) Int. Immunopharmacol., 6, pp. 1634-1641
- Leung, M.Y., Liu, C., Koon, L.C., Fung, K.P., (2006) Immunol. Lett., 105, pp. 101-114
- Li, X., Xu, W., (2010) J. Ethnopharmacol., 135, pp. 1-6
- Shao, B.M., Dai, H., Xu, W., Lin, Z., Gao, X.M., (2004) Biochem. Biophys. Res. Commun., 323, pp. 133-141
Citas:
---------- APA ----------
Pérez-Recalde, M., Matulewicz, M.C., Pujol, C.A. & Carlucci, M.J.
(2014)
. In vitro and in vivo immunomodulatory activity of sulfated polysaccharides from red seaweed Nemalion helminthoides. International Journal of Biological Macromolecules, 63, 38-42.
http://dx.doi.org/10.1016/j.ijbiomac.2013.10.024---------- CHICAGO ----------
Pérez-Recalde, M., Matulewicz, M.C., Pujol, C.A., Carlucci, M.J.
"In vitro and in vivo immunomodulatory activity of sulfated polysaccharides from red seaweed Nemalion helminthoides"
. International Journal of Biological Macromolecules 63
(2014) : 38-42.
http://dx.doi.org/10.1016/j.ijbiomac.2013.10.024---------- MLA ----------
Pérez-Recalde, M., Matulewicz, M.C., Pujol, C.A., Carlucci, M.J.
"In vitro and in vivo immunomodulatory activity of sulfated polysaccharides from red seaweed Nemalion helminthoides"
. International Journal of Biological Macromolecules, vol. 63, 2014, pp. 38-42.
http://dx.doi.org/10.1016/j.ijbiomac.2013.10.024---------- VANCOUVER ----------
Pérez-Recalde, M., Matulewicz, M.C., Pujol, C.A., Carlucci, M.J. In vitro and in vivo immunomodulatory activity of sulfated polysaccharides from red seaweed Nemalion helminthoides. Int. J. Biol. Macromol. 2014;63:38-42.
http://dx.doi.org/10.1016/j.ijbiomac.2013.10.024