Artículo

Salinas, F.M.; Vázquez, L.; Gentilini, M.V.; O´Donohoe, A.; Regueira, E.; Nabaes Jodar, M.S.; Viegas, M.; Michelini, F.M.; Hermida, G.; Alché, L.E.; Bueno, C.A. "Aesculus hippocastanum L. seed extract shows virucidal and antiviral activities against respiratory syncytial virus (RSV) and reduces lung inflammation in vivo" (2019) Antiviral Research. 164:1-11
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract disease and bronchiolitis in children worldwide. No vaccine or specific, effective treatment is currently available. β-escin is one of the main bioactive constituents of Aesculus hippocastanum L. (Hippocastanaceae) seed extract (AH), and both β-escin and AH have demonstrated a beneficial role in clinical therapy because of their anti-edematous, anti-inflammatory and antioxidative effects. Besides, we have reported that β-escin and AH show virucidal, antiviral and immunomodulatory activities against the enveloped viruses HSV-1, VSV and Dengue virus in vitro. In this study, we demonstrate that β-escin and AH have virucidal and antiviral activities against RSV, as well as NF-κB, AP-1 and cytokine modulating activities in RSV infected epithelial and macrophage cell lines in vitro. Besides, in a murine model of pulmonary RSV infection, AH treatment improves the course of acute disease, evidenced by decreased weight loss, reduced RSV lung titers, and attenuated airway inflammation. In contrast, even though β-escin showed, similarly to AH, antiviral and immunomodulatory properties in vitro, it neither reduces viral titers nor attenuates lung injury in vivo. Thus, our data demonstrate that AH restrains RSV disease through antiviral and immunomodulatory effect. © 2019 Elsevier B.V.

Registro:

Documento: Artículo
Título:Aesculus hippocastanum L. seed extract shows virucidal and antiviral activities against respiratory syncytial virus (RSV) and reduces lung inflammation in vivo
Autor:Salinas, F.M.; Vázquez, L.; Gentilini, M.V.; O´Donohoe, A.; Regueira, E.; Nabaes Jodar, M.S.; Viegas, M.; Michelini, F.M.; Hermida, G.; Alché, L.E.; Bueno, C.A.
Filiación:Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Laboratorio de Virología, Buenos Aires, Argentina
CONICET, Universidad de Buenos Aires, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires, Argentina
Unidad Operativa Centro de Contención Biológica (UOCCB), Administración Nacional de Laboratorios e Institutos de Salud (ANLIS), Argentina
Instituto de Medicina Traslacional, Trasplante y Bioingeniería (IMETTYB)-CONICET, Buenos Aires, Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Laboratorio de Biología de Anfibios-Histología Animal, Buenos Aires, Argentina
CONICET, Buenos Aires, Argentina
Laboratorio de Virología, Hospital de Niños Ricardo Gutierrez, Buenos Aires, Argentina
Ministerio de Salud de la Ciudad de, Buenos Aires, Argentina
Palabras clave:Aesculus hippocastanum; Antiviral; Immunomodulatory; Medicinal plants; Respiratory syncytial virus (RSV); β-escin; Aesculus hippocastanum extract; beta actin; beta escin; gamma interferon; immunoglobulin enhancer binding protein; interleukin 17; interleukin 4; interleukin 6; interleukin 8; transcription factor AP 1; tumor necrosis factor; virus RNA; Aesculus hippocastanum; animal cell; animal experiment; animal model; animal tissue; antiviral activity; Article; controlled study; epithelium cell; human; human cell; immunomodulation; in vitro study; lung epithelium; lung parenchyma; macrophage; mouse; nonhuman; plant seed; priority journal; respiratory syncytial virus infection; respiratory tract inflammation; RNA replication; signal transduction; Th2 cell; treatment duration; Vero cell line; virucidal activity; virus infectivity; virus load
Año:2019
Volumen:164
Página de inicio:1
Página de fin:11
DOI: http://dx.doi.org/10.1016/j.antiviral.2019.01.018
Título revista:Antiviral Research
Título revista abreviado:Antiviral Res.
ISSN:01663542
CODEN:ARSRD
CAS:beta escin, 64156-26-9; gamma interferon, 82115-62-6; interleukin 8, 114308-91-7
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_01663542_v164_n_p1_Salinas

Referencias:

  • Borg, I., Rohde, G., Löseke, S., Bittscheidt, J., Schultze-Werninghaus, G., Stephan, V., Bufe, A., Evaluation of a quantitative real-time PCR for the detection of respiratory syncytial virus in pulmonary diseases (2003) Eur. Respir. J., 21 (6), pp. 944-951
  • Bueno, C.A., Michelini, F.M., Pertino, M.W., Gómez, C.A., Schmeda-Hirschmann, G., Alché, L.E., Natural and semisynthetic diterpenoids with antiviral and immunomodulatory activities block the ERK signalling pathway (2015) Med. Microbiol. Immunol., 204 (5), pp. 575-584
  • Caidi, H., Harcourt, J.L., Haynes, L.M., RSV growth and quantification by microtitration and qRT-PCR assays (2016) Methods Mol. Biol., 1442, pp. 13-32
  • Challa, S., Scott, A.D., Yuzhakov, O., Zhou, Y., Tiong-Yip, C.L., Gao, N., Thresher, J., Yu, Q., Mechanism of action for respiratory syncytial virus inhibitor RSV604 (2015) Antimicrob. Agents Chemother., 59 (2), pp. 1080-1087
  • Cheng, Y., Wang, H., Mao, M., Liang, C., Zhang, Y., Yang, D., Wei, Z., Cai, Q., Escin increases the survival rate of LPS-induced septic mice through inhibition of HMGB1 release from macrophages (2015) Cell. Physiol. Biochem., 36 (4), pp. 1577-1586
  • Christiaansen, A., Varga, S.M., Spencer, J.V., Viral manipulation of the host immune response (2015) Curr. Opin. Immunol., 36, pp. 54-60
  • Dawson-Caswell, M., Muncie, H.L., Jr., Respiratory syncytial virus infection in children (2011) Am. Fam. Physician, 83, pp. 141-146
  • Dey, N., Liu, T., Garofalo, R.P., Casola, A., TAK1 regulates NF-ΚB and AP-1 activation in airway epithelial cells following RSV infection (2011) Virology, 418 (2), pp. 93-101. , 30
  • Domanski, D., Zegrocka-Stendel, O., Perzanowska, A., Dutkiewicz, M., Kowalewska, M., Grabowska, I., Maciejko, D., Koziak, K., Molecular mechanism for cellular response to β-escin and its therapeutic implications (2016) PLoS One, 11 (10). , 2016 Oct 11
  • European Medicines Agency (EMA), Assessment Report on Aesculus Hippocastanum L. (2009), https://www.ema.europa.eu/documents/herbal-report/assessment-report-aesculus-hippocastanum-l-semen_en.pdf, Evaluation of Medicines for Human Use Semen. Doc. Ref.: EMEA/HMPC/225304/2008 (Accessed 10 October 2018); Hall, C.J., Boyle, R.H., Astin, J.W., Flores, M.V., Oehlers, S.H., Sanderson, L.E., Ellett, F., Crosier, P.S., Immunoresponsive gene 1 augments bactericidal activity of macrophage-lineage cells by regulating beta-oxidationdependent mitochondrial ROS production (2013) Cell Metabol., 18, pp. 265-278
  • Hassan, S.T., Masarčíková, R., Berchová, K., Bioactive natural products with anti-herpes simplex virus properties (2015) J. Pharm. Pharmacol., 67 (10), pp. 1325-1336. , Epub 2015 Jun 9
  • Kim, C., Ahmed, J.A., Eidex, R.B., Nyoka, R., Waiboci, L.W., Erdman, D., Comparison of nasopharyngeal and oropharyngeal swabs for the diagnosis of eight respiratory viruses by real-time reverse transcription- PCR assays (2011) PLoS One, 6 (6). , 2011 Jun 30
  • Krishnamoorthy, N., Khare, A., Oriss, T.B., Raundhal, M., Morse, C., Yarlagadda, M., Wenzel, S.E., Ray, P., Early infection with respiratory syncytial virus impairs regulatory T cell function and increases susceptibility to allergic asthma (2012) Nat. Med., 18, pp. 1525-1530
  • Kwon, Y.S., Park, S.H., Kim, M.A., Kim, H.J., Park, J.S., Lee, M.Y., Lee, C.W., Choi, W.I., Risk of mortality associated with respiratory syncytial virus and influenza infection in adults (2017) BMC Infect. Dis., 17 (1), p. 785. , 20
  • Larrañaga, C.L., Ampuero, S.L., Luchsinger, V.F., Carrión, F.A., Aguilar, N.V., Morales, P.R., Palomino, M.A., Avendaño, L.F., Impaired immune response in severe human lower tract respiratory infection by respiratory syncytial virus (2009) Pediatr. Infect. Dis. J., 28, pp. 867-873
  • Li, X.M., Sun, S.Z., Wu, F.L., Shi, T., Fan, H.J., Li, D.Z., Study on JNK/AP-1 signalling pathway of airway mucus hypersecretion of severe pneumonia under RSV infection (2016) Eur. Rev. Med. Pharmacol. Sci., 20 (5), pp. 853-857
  • Lindner, I., Meier, C., Url, A., Unger, H., Grassauer, A., Prieschl-Grassauer, E., Doerfler, P., Beta-escin has potent anti-allergic efficacy and reduces allergic airway inflammation (2010) BMC Immunol., 21, pp. 11-24
  • Liu, S., Wang, H., Qiu, C., Zhang, J., Zhang, T., Zhou, W., Lu, Z., Liu, Z., Escin inhibits lipopolysaccharide-induced inflammation in human periodontal ligament cells (2012) Mol. Med. Rep., 6 (5), pp. 1150-1154
  • Lukacs, N.W., Moore, M.L., Rudd, B.D., Berlin, A.A., Collins, R.D., Olson, S.J., Ho, S.B., Peebles, R.S., Jr., Differential immune responses and pulmonary pathophysiology are induced by two different strains of respiratory syncytial virus (2006) Am. J. Pathol., 169 (3), pp. 977-986
  • Markus, M.A., Dullin, C., Mitkovski, M., Prieschl-Grassauer, E., Epstein, M.M., Alves, F., Non-invasive optical imaging of eosinophilia during the course of an experimental allergic airways disease model and in response to therapy (2014) PLoS One, 9 (2). , 25
  • Masaki, T., Kojima, T., Okabayashi, T., Ogasawara, N., Ohkuni, T., Obata, K., Takasawa, A., Sawada, N., A nuclear factor-κB signaling pathway via protein kinase C δ regulates replication of respiratory syncytial virus in polarized normal human nasal epithelial cells (2011) Mol. Biol. Cell, 22 (13), pp. 2144-2156. , 1
  • Michelini, F.M., Alché, L.E., Bueno, C.A., Virucidal, antiviral and immunomodulatory activities of β-escin and Aesculus hippocastanum extract (2018) J. Pharm. Pharmacol.
  • Nair, H., Nokes, D.J., Gessner, B.D., Dherani, M., Madhi, S.A., Singleton, R.J., O'Brien, K.L., Campbell, H., Global burden of acute lower respiratory infections due to respiratory syncytial virus in young children: a systematic review and meta-analysis (2010) Lancet, 375 (9725), pp. 1545-1555. , 1
  • Piedimonte, G., Perez, M.K., Respiratory syncytial virus infection and bronchiolitis (2014) Pediatr. Rev., 35 (12), pp. 519-530
  • Pittler, M.H., Ernst, E., Horse chestnut seed extract for chronic venous insufficiency (2012) Cochrane Database Syst. Rev., 11
  • Rudd, P.A., Chen, W., Mahalingam, S., Mouse and cotton rat models of human respiratory syncytial virus (2016) Methods Mol. Biol., 1442, pp. 209-217
  • Saravia, J., You, D., Shrestha, B., Jaligama, S., Siefker, D., Lee, G.I., Respiratory Syncytial Virus Disease Is Mediated by Age-Variable IL-33 (2015) PLoS Pathog., 11 (10)
  • Shi, H., Ren, K., L, B., Zhang, W., Zhao, Y., Tan, R.X., Li, E., Baicalin from Scutellaria baicalensis blocks respiratory syncytial virus (RSV) infection and reduces inflammatory cell infiltration and lung injury in mice (2016) Sci. Rep., 21 (6), p. 35851
  • Sigurs, N., Aljassim, F., Kjellman, B., Robinson, P.D., Sigurbergsson, F., Bjarnason, R., Gustafsson, P.M., Asthma and allergy patterns over 18 years after severe RSV bronchiolitis in the first year of life (2010) Thorax, 65, pp. 1045-1052
  • Sirtori, C.R., Aescin: pharmacology, pharmacokinetics and therapeutic profile (2001) Pharmacol. Res., 44, pp. 183-193
  • Stokes, K.L., Chi, M.H., Sakamoto, K., Newcomb, D.C., Currier, M.G., Huckabee, M.M., Lee, S., Moore, M.L., Differential pathogenesis of respiratory syncytial virus clinical isolates in BALB/c mice (2011) J. Virol., 85 (12), pp. 5782-5793
  • Tian, B., Yang, J., Zhao, Y., Ivanciuc, T., Sun, H., Wakamiya, M., Garofalo, R.P., Brasier, A.R., Central role of the NF-κB pathway in the scgb1a1-expressing epithelium in mediating respiratory syncytial virus-induced airway inflammation (2018) J. Virol., 92 (11). , 14 pii: e00441-18
  • Tregoning, J.S., Schwarze, J., Respiratory viral infections in infants: causes, clinical symptoms, virology, and immunology (2010) Clin. Microbiol. Rev., 23, pp. 74-98
  • Turner, T.L., Kopp, B.T., Paul, G., Landgrave, L.C., Hayes, D., Jr., Thompson, R., Respiratory syncytial virus: current and emerging treatment options (2014) Clinicoecon Outcomes Res., 25 (6), pp. 217-225
  • Woolums, A., Lee, S., Moore, M., Animal models of respiratory syncytial virus pathogenesis and vaccine development: opportunities and future directions (2011) Human Respiratory Syncytial Virus Infection, , B. Resch InTech
  • Xin, W., Zhang, L., Sun, F., Jiang, N., Fan, H., Wang, T., Li, Z., Fu, F., Escin exerts synergistic anti-inflammatory effects with low doses of glucocorticoids in vivo and in vitro (2011) Phytomedicine, 18 (4), pp. 272-277. , 15

Citas:

---------- APA ----------
Salinas, F.M., Vázquez, L., Gentilini, M.V., O´Donohoe, A., Regueira, E., Nabaes Jodar, M.S., Viegas, M.,..., Bueno, C.A. (2019) . Aesculus hippocastanum L. seed extract shows virucidal and antiviral activities against respiratory syncytial virus (RSV) and reduces lung inflammation in vivo. Antiviral Research, 164, 1-11.
http://dx.doi.org/10.1016/j.antiviral.2019.01.018
---------- CHICAGO ----------
Salinas, F.M., Vázquez, L., Gentilini, M.V., O´Donohoe, A., Regueira, E., Nabaes Jodar, M.S., et al. "Aesculus hippocastanum L. seed extract shows virucidal and antiviral activities against respiratory syncytial virus (RSV) and reduces lung inflammation in vivo" . Antiviral Research 164 (2019) : 1-11.
http://dx.doi.org/10.1016/j.antiviral.2019.01.018
---------- MLA ----------
Salinas, F.M., Vázquez, L., Gentilini, M.V., O´Donohoe, A., Regueira, E., Nabaes Jodar, M.S., et al. "Aesculus hippocastanum L. seed extract shows virucidal and antiviral activities against respiratory syncytial virus (RSV) and reduces lung inflammation in vivo" . Antiviral Research, vol. 164, 2019, pp. 1-11.
http://dx.doi.org/10.1016/j.antiviral.2019.01.018
---------- VANCOUVER ----------
Salinas, F.M., Vázquez, L., Gentilini, M.V., O´Donohoe, A., Regueira, E., Nabaes Jodar, M.S., et al. Aesculus hippocastanum L. seed extract shows virucidal and antiviral activities against respiratory syncytial virus (RSV) and reduces lung inflammation in vivo. Antiviral Res. 2019;164:1-11.
http://dx.doi.org/10.1016/j.antiviral.2019.01.018