Artículo

Patriarca, A.; da Cruz Cabral, L.; Pavicich, M.A.; Nielsen, K.F.; Andersen, B."Secondary metabolite profiles of small-spored Alternaria support the new phylogenetic organization of the genus" (2019) International Journal of Food Microbiology. 291:135-143
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Abstract:

The group of the small-spored Alternaria species is particularly relevant in foods due to its high frequency and wide distribution in different crops. These species are responsible for the accumulation of mycotoxins and bioactive secondary metabolites in food. The taxonomy of the genus has been recently revised with particular attention on them; several morphospecies within this group cannot be segregated by phylogenetic methods, and the most recent classifications proposed to elevate several phylogenetic species-groups to the taxonomic status of section. The purpose of the present study was to compare the new taxonomic revisions in Alternaria with secondary metabolite profiles with special focus on sections Alternaria and Infectoriae and food safety. A total of 360 small-spored Alternaria isolates from Argentinean food crops (tomato fruit, pepper fruit, blueberry, apple, wheat grain, walnut, pear, and plum) was morphologically identified to species-group according to Simmons (2007), and their secondary metabolite profile was determined. The isolates belonged to A. infectoria sp.-grp. (19), A. tenuissima sp.-grp. (262), A. arborescens sp.-grp. (40), and A. alternata sp.-grp. (7); 32 isolates, presenting characteristics overlapping between the last three groups, were classified as Alternaria sp. A high chemical diversity was observed; 78 different metabolites were detected, 31 of them of known chemical structure. The isolates from A. infectoria sp.-grp. (=Alternaria section Infectoriae) presented a specific secondary metabolite profile, different from the other species-groups. Infectopyrones, novae-zelandins and phomapyrones were the most frequent metabolites produced by section Infectoriae. Altertoxin-I and alterperylenol were the only compounds that these isolates produced in common with members of section Alternaria. None of the well-known Alternaria toxins, considered relevant in foods, namely alternariol (AOH), alternariol monomethyl ether (AME), tenuazonic acid (TeA), tentoxin (TEN) or altenuene (ALT), were produced by isolates of this section. On the other hand, strains from section Alternaria (A. tenuissima, A. arborescens, and A. alternata sp.-grps.) shared a common metabolite profile, indistinguishable from each other. AOH, AME, ALT, TEN, and TeA were the most frequently mycotoxins produced, together with pyrenochaetic acid A and altechromone A. Alternaria section Alternaria represents a substantial risk in food, since their members in all types of crops are able to produce the toxic metabolites. © 2018 Elsevier B.V.

Registro:

Documento: Artículo
Título:Secondary metabolite profiles of small-spored Alternaria support the new phylogenetic organization of the genus
Autor:Patriarca, A.; da Cruz Cabral, L.; Pavicich, M.A.; Nielsen, K.F.; Andersen, B.
Filiación:Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Orgánica, Laboratorio de Microbiología de Alimentos. CONICET, Instituto de Micología y Botánica (INMIBO), Buenos Aires, Argentina
Department of Biotechnology and Biomedicine, Technical University of Denmark, Kgs. Lyngby, DK-2800, Denmark
Palabras clave:Alternaria; Food safety; Secondary metabolite profiles; Section Alternaria; Section Infectoriae; altenuene; alternariol; alternariol monomethyl ether; alterperylenol; altertoxin i; infectopyrone derivative; mycotoxin; natural product; novae zelandin derivative; phomapyrone derivative; tentoxin; tenuazonic acid; unclassified drug; cyclopeptide; lactone; mycotoxin; tenuazonic acid; Alternaria; Alternaria alternata; Alternaria arborescens; Alternaria infectoria; Alternaria tenuissima; apple; Article; blueberry; food crop; food safety; fungal strain; fungus isolation; genus; grain; metabolite; morphospecies; nonhuman; pear; pepper; phylogeny; plum; tea; tomato; walnut; wheat; Alternaria; analysis; Argentina; black pepper; classification; crop; food contamination; food control; fruit; Malus; metabolism; microbiology; Pyrus; secondary metabolism; Alternaria; Argentina; Blueberry Plants; Crops, Agricultural; Food Contamination; Food Microbiology; Fruit; Juglans; Lactones; Lycopersicon esculentum; Malus; Mycotoxins; Peptides, Cyclic; Phylogeny; Piper nigrum; Prunus domestica; Pyrus; Secondary Metabolism; Tenuazonic Acid; Triticum
Año:2019
Volumen:291
Página de inicio:135
Página de fin:143
DOI: http://dx.doi.org/10.1016/j.ijfoodmicro.2018.11.022
Handle:http://hdl.handle.net/20.500.12110/paper_01681605_v291_n_p135_Patriarca
Título revista:International Journal of Food Microbiology
Título revista abreviado:Int. J. Food Microbiol.
ISSN:01681605
CODEN:IJFMD
CAS:alternariol, 641-38-3; tenuazonic acid, 610-88-8, 75652-74-3; lactone, 1338-03-0; altenuene; alternariol; alternariol monomethyl ether; Lactones; Mycotoxins; Peptides, Cyclic; tentoxin; Tenuazonic Acid
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681605_v291_n_p135_Patriarca

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Citas:

---------- APA ----------
Patriarca, A., da Cruz Cabral, L., Pavicich, M.A., Nielsen, K.F. & Andersen, B. (2019) . Secondary metabolite profiles of small-spored Alternaria support the new phylogenetic organization of the genus. International Journal of Food Microbiology, 291, 135-143.
http://dx.doi.org/10.1016/j.ijfoodmicro.2018.11.022
---------- CHICAGO ----------
Patriarca, A., da Cruz Cabral, L., Pavicich, M.A., Nielsen, K.F., Andersen, B. "Secondary metabolite profiles of small-spored Alternaria support the new phylogenetic organization of the genus" . International Journal of Food Microbiology 291 (2019) : 135-143.
http://dx.doi.org/10.1016/j.ijfoodmicro.2018.11.022
---------- MLA ----------
Patriarca, A., da Cruz Cabral, L., Pavicich, M.A., Nielsen, K.F., Andersen, B. "Secondary metabolite profiles of small-spored Alternaria support the new phylogenetic organization of the genus" . International Journal of Food Microbiology, vol. 291, 2019, pp. 135-143.
http://dx.doi.org/10.1016/j.ijfoodmicro.2018.11.022
---------- VANCOUVER ----------
Patriarca, A., da Cruz Cabral, L., Pavicich, M.A., Nielsen, K.F., Andersen, B. Secondary metabolite profiles of small-spored Alternaria support the new phylogenetic organization of the genus. Int. J. Food Microbiol. 2019;291:135-143.
http://dx.doi.org/10.1016/j.ijfoodmicro.2018.11.022