Artículo

Pavan, M.E.; Venero, E.S.; Egoburo, D.E.; Pavan, E.E.; López, N.I.; Julia Pettinari, M. "Glycerol inhibition of melanin biosynthesis in the environmental Aeromonas salmonicida 34mel T" (2019) Applied Microbiology and Biotechnology. 103(4):1865-1876
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Abstract:

The environmental strain Aeromonas salmonicida subsp. pectinolytica 34mel T produces abundant melanin through the homogentisate pathway in several culture media, but unexpectedly not when grown in a medium containing glycerol. Using this observation as a starting point, this study investigated the underlying causes of the inhibition of melanin synthesis by glycerol, to shed light on factors that affect melanin production in this microorganism. The effect of different carbon sources on melanin formation was related to the degree of oxidation of their C atoms, as the more reduced substrates delayed melanization more than the more oxidized ones, although only glycerol completely abolished melanin production. Glyphosate, an inhibitor of aromatic amino acid synthesis, did not affect melanization, while bicyclopyrone, an inhibitor of 4-hydroxyphenylpyruvate dioxygenase (Hpd), the enzyme responsible for the synthesis of homogentisate, prevented melanin synthesis. These results showed that melanin production in 34mel T depends on the degradation of aromatic amino acids from the growth medium and not on de novo aromatic amino acid synthesis. The presence of glycerol changed the secreted protein profile, but none of the proteins affected could be directly connected with melanin synthesis or transport. Transcription analysis of hpd, encoding the key enzyme for melanin synthesis, showed a clear inhibition caused by glycerol. The results obtained in this work indicate that a significant decrease in the transcription of hpd, together with a more reduced intracellular state, would lead to the abolishment of melanin synthesis observed. The effect of glycerol on melanization can thus be attributed to a combination of metabolic and regulatory effects. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.

Registro:

Documento: Artículo
Título:Glycerol inhibition of melanin biosynthesis in the environmental Aeromonas salmonicida 34mel T
Autor:Pavan, M.E.; Venero, E.S.; Egoburo, D.E.; Pavan, E.E.; López, N.I.; Julia Pettinari, M.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
IQUIBICEN-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Biomedical Technologies Laboratory, Department of Electronics, Information and Bioengineering, Politecnico di Milano, Milan, Italy
Palabras clave:4-Hydroxyphenylpyruvate dioxygenase; Aeromonas salmonicida subsp. pectinolytica; Glycerol; Homogentisate pathway; hpd expression; Melanin inhibition; Amino acids; Aromatic compounds; Aromatization; Biochemistry; Enzyme inhibition; Glycerol; Melanin; Substrates; Transcription; 4-Hydroxyphenylpyruvate dioxygenase; Aeromonas salmonicida; Different carbon sources; Environmental strain; Homogentisate; hpd expression; Melanin biosynthesis; Transcription analysis; Biosynthesis; Aeromonas salmonicida; Aeromonas salmonicida subsp. pectinolytica
Año:2019
Volumen:103
Número:4
Página de inicio:1865
Página de fin:1876
DOI: http://dx.doi.org/10.1007/s00253-018-9545-9
Título revista:Applied Microbiology and Biotechnology
Título revista abreviado:Appl. Microbiol. Biotechnol.
ISSN:01757598
CODEN:AMBID
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_01757598_v103_n4_p1865_Pavan

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Citas:

---------- APA ----------
Pavan, M.E., Venero, E.S., Egoburo, D.E., Pavan, E.E., López, N.I. & Julia Pettinari, M. (2019) . Glycerol inhibition of melanin biosynthesis in the environmental Aeromonas salmonicida 34mel T. Applied Microbiology and Biotechnology, 103(4), 1865-1876.
http://dx.doi.org/10.1007/s00253-018-9545-9
---------- CHICAGO ----------
Pavan, M.E., Venero, E.S., Egoburo, D.E., Pavan, E.E., López, N.I., Julia Pettinari, M. "Glycerol inhibition of melanin biosynthesis in the environmental Aeromonas salmonicida 34mel T" . Applied Microbiology and Biotechnology 103, no. 4 (2019) : 1865-1876.
http://dx.doi.org/10.1007/s00253-018-9545-9
---------- MLA ----------
Pavan, M.E., Venero, E.S., Egoburo, D.E., Pavan, E.E., López, N.I., Julia Pettinari, M. "Glycerol inhibition of melanin biosynthesis in the environmental Aeromonas salmonicida 34mel T" . Applied Microbiology and Biotechnology, vol. 103, no. 4, 2019, pp. 1865-1876.
http://dx.doi.org/10.1007/s00253-018-9545-9
---------- VANCOUVER ----------
Pavan, M.E., Venero, E.S., Egoburo, D.E., Pavan, E.E., López, N.I., Julia Pettinari, M. Glycerol inhibition of melanin biosynthesis in the environmental Aeromonas salmonicida 34mel T. Appl. Microbiol. Biotechnol. 2019;103(4):1865-1876.
http://dx.doi.org/10.1007/s00253-018-9545-9