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Abstract:

Acid-sensing ion channels (ASICs) regulate synaptic activities and play important roles in neurodegenerative diseases. We found that these channels can be activated in neurons of the medial nucleus of the trapezoid body (MNTB) of the auditory system in the CNS. A drop in extracellular pH induces transient inward ASIC currents (IASICs) in postsynaptic MNTB neurons from wild-type mice. The inhibition of IASICs by psalmotoxin-1 (PcTx1) and the absence of these currents in knock-out mice for ASIC-1a subunit (ASIC1a-/-) suggest that homomeric ASIC-1as are mediating these currents in MNTB neurons. Furthermore, we detect ASIC1a-dependent currents during synaptic transmission, suggesting an acidification of the synaptic cleft due to the corelease of neurotransmitter and H+ from synaptic vesicles. These currents are capable of eliciting action potentials in the absence of glutamatergic currents. A significant characteristic of these homomeric ASIC-1as is their permeability to Ca2+. Activation of ASIC-1a in MNTB neurons by exogenous H+ induces an increase in intracellular Ca2+. Furthermore, the activation of postsynaptic ASIC-1as during high-frequency stimulation (HFS) of the presynaptic nerve terminal leads to a PcTx1-sensitive increase in intracellular Ca2+ in MNTB neurons, which is independent of glutamate receptors and is absent in neurons from ASIC1a-/-mice. During HFS, the lack of functional ASICs in synaptic transmission results in an enhanced short-term depression of glutamatergic EPSCs. These results strongly support the hypothesis of protons as neurotransmitters and demonstrate that presynaptic released protons modulate synaptic transmission by activating ASIC-1as at the calyx of Held-MNTB synapse. © 2017 the authors.

Registro:

Documento: Artículo
Título:Activated by evoked released protons modulate synaptic transmission at the mouse calyx of held synapse
Autor:González-Inchauspe, C.; Urbano, F.J.; Di Guilmi, M.N.; Uchitel, O.D.
Filiación:Instituto de Fisiología Biología molecular y Neurociencias, Departamento de Fisiología Biología Molecular y Celular “Dr Héctor Maldonado”, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, EGA, Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, Beunos Aires, CP 1428, Argentina
Palabras clave:ASIC-1a; Calyx of held; Glutamatergic EPSCs; Protons; Short-term depression; Synaptic plasticity; acid sensing ion channel; amiloride; AMPA receptor; antiporter; creatine phosphate; glutamate receptor; glutamic acid; postsynaptic receptor; Accn2 protein, mouse; acid sensing ion channel; proton; acidification; action potential; animal experiment; Article; cell membrane permeability; depolarization; depression; desensitization; drug mechanism; electrophysiology; evoked response; female; fluorescence; glutamatergic synapse; male; mouse; nerve cell plasticity; nerve ending; neurotransmitter release; nonhuman; patch clamp technique; priority journal; synapse; synaptic transmission; animal; auditory evoked potential; C57BL mouse; channel gating; chemistry; cochlear nucleus; metabolism; pH; physiology; synapse; synaptic transmission; Acid Sensing Ion Channels; Animals; Cochlear Nucleus; Evoked Potentials, Auditory; Female; Hydrogen-Ion Concentration; Ion Channel Gating; Male; Mice; Mice, Inbred C57BL; Neuronal Plasticity; Protons; Synapses; Synaptic Transmission
Año:2017
Volumen:37
Número:10
Página de inicio:2589
Página de fin:2599
DOI: http://dx.doi.org/10.1523/JNEUROSCI.2566-16.2017
Título revista:Journal of Neuroscience
Título revista abreviado:J. Neurosci.
ISSN:02706474
CODEN:JNRSD
CAS:amiloride, 2016-88-8, 2609-46-3; creatine phosphate, 67-07-2; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; proton, 12408-02-5, 12586-59-3; Accn2 protein, mouse; Acid Sensing Ion Channels; Protons
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_02706474_v37_n10_p2589_GonzalezInchauspe

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Citas:

---------- APA ----------
González-Inchauspe, C., Urbano, F.J., Di Guilmi, M.N. & Uchitel, O.D. (2017) . Activated by evoked released protons modulate synaptic transmission at the mouse calyx of held synapse. Journal of Neuroscience, 37(10), 2589-2599.
http://dx.doi.org/10.1523/JNEUROSCI.2566-16.2017
---------- CHICAGO ----------
González-Inchauspe, C., Urbano, F.J., Di Guilmi, M.N., Uchitel, O.D. "Activated by evoked released protons modulate synaptic transmission at the mouse calyx of held synapse" . Journal of Neuroscience 37, no. 10 (2017) : 2589-2599.
http://dx.doi.org/10.1523/JNEUROSCI.2566-16.2017
---------- MLA ----------
González-Inchauspe, C., Urbano, F.J., Di Guilmi, M.N., Uchitel, O.D. "Activated by evoked released protons modulate synaptic transmission at the mouse calyx of held synapse" . Journal of Neuroscience, vol. 37, no. 10, 2017, pp. 2589-2599.
http://dx.doi.org/10.1523/JNEUROSCI.2566-16.2017
---------- VANCOUVER ----------
González-Inchauspe, C., Urbano, F.J., Di Guilmi, M.N., Uchitel, O.D. Activated by evoked released protons modulate synaptic transmission at the mouse calyx of held synapse. J. Neurosci. 2017;37(10):2589-2599.
http://dx.doi.org/10.1523/JNEUROSCI.2566-16.2017