Artículo

Zubeldia-Brenner, L.; Gutierrez-Uzquiza, A.; Barrio-Real, L.; Wang, H.; Kazanietz, M.G.; Leskow, F.C. "β3-Chimaerin, a novel member of the chimaerin Rac-GAP family" (2014) Molecular Biology Reports:1-10
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Abstract:

Chimaerins are a family of diacylglycerol- and phorbol ester-regulated GTPase activating proteins (GAPs) for the small G-protein Rac. Extensive evidence indicates that these proteins play important roles in development, axon guidance, metabolism, cell motility, and T cell activation. Four isoforms have been reported to-date, which are products of CHN1 (α1- and α2-chimaerins) and CHN2 (β1- and β2-chimaerins) genes. Although these gene products are assumed to be generated by alternative splicing, bioinformatics analysis of the CHN2 gene revealed that β1- and β2-chimaerins are the products of alternative transcription start sites (TSSs) in different promoter regions. Furthermore, we found an additional TSS in CHN2 gene that leads to a novel product, which we named β3-chimaerin. Expression profile analysis revealed predominantly low levels for the β3-chimaerin transcript, with higher expression levels in epididymis, plasma blood leucocytes, spleen, thymus, as well as various areas of the brain. In addition to the prototypical SH2, C1, and Rac-GAP domains, β3-chimaerin has a unique N-terminal domain. Studies in cells established that β3-chimaerin has Rac-GAP activity and is responsive to phorbol esters. The enhanced responsiveness of β3-chimaerin for phorbol ester-induced translocation relative to β2-chimaerin suggests differential ligand accessibility to the C1 domain. © 2014 Springer Science+Business Media Dordrecht.

Registro:

Documento: Artículo
Título:β3-Chimaerin, a novel member of the chimaerin Rac-GAP family
Autor:Zubeldia-Brenner, L.; Gutierrez-Uzquiza, A.; Barrio-Real, L.; Wang, H.; Kazanietz, M.G.; Leskow, F.C.
Filiación:Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, IQUIBICEN-CONICET, Universidad de Buenos Aires, Buenos Aires, C1428EGA, Argentina
Department of Pharmacology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, 19104-6160, United States
Departamento de Ciencias Básicas, Universidad Nacional de Luján, Luján, 6700, Argentina
Idioma: Inglés
Palabras clave:C1 domain; Chimaerin; CHN2; Phorbol esters; Rac-GAP
Año:2014
Página de inicio:1
Página de fin:10
DOI: http://dx.doi.org/10.1007/s11033-014-3055-3
Título revista:Molecular Biology Reports
Título revista abreviado:Mol. Biol. Rep.
ISSN:03014851
CODEN:MLBRB
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_03014851_v_n_p1_ZubeldiaBrenner

Citas:

---------- APA ----------
Zubeldia-Brenner, L., Gutierrez-Uzquiza, A., Barrio-Real, L., Wang, H., Kazanietz, M.G. & Leskow, F.C. (2014) . β3-Chimaerin, a novel member of the chimaerin Rac-GAP family. Molecular Biology Reports, 1-10.
http://dx.doi.org/10.1007/s11033-014-3055-3
---------- CHICAGO ----------
Zubeldia-Brenner, L., Gutierrez-Uzquiza, A., Barrio-Real, L., Wang, H., Kazanietz, M.G., Leskow, F.C. "β3-Chimaerin, a novel member of the chimaerin Rac-GAP family" . Molecular Biology Reports (2014) : 1-10.
http://dx.doi.org/10.1007/s11033-014-3055-3
---------- MLA ----------
Zubeldia-Brenner, L., Gutierrez-Uzquiza, A., Barrio-Real, L., Wang, H., Kazanietz, M.G., Leskow, F.C. "β3-Chimaerin, a novel member of the chimaerin Rac-GAP family" . Molecular Biology Reports, 2014, pp. 1-10.
http://dx.doi.org/10.1007/s11033-014-3055-3
---------- VANCOUVER ----------
Zubeldia-Brenner, L., Gutierrez-Uzquiza, A., Barrio-Real, L., Wang, H., Kazanietz, M.G., Leskow, F.C. β3-Chimaerin, a novel member of the chimaerin Rac-GAP family. Mol. Biol. Rep. 2014:1-10.
http://dx.doi.org/10.1007/s11033-014-3055-3