Artículo

Marin, M.S.; Hecker, Y.P.; Quintana, S.; Pérez, S.E.; Leunda, M.R.; Cantón, G.J.; Cobo, E.R.; Moore, D.P.; Odeón, A.C."Immunization with inactivated antigens of Neospora caninum induces toll-like receptors 3, 7, 8 and 9 in maternal-fetal interface of infected pregnant heifers" (2017) Veterinary Parasitology. 243:12-17
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Abstract:

Neospora caninum is an obligate parasite and a major cause of abortion in cattle. Pregnancy failures appear to be associated with weak innate defences on the maternal-fetal interface during infection with N. caninum. Herein, we studied the gene expression of Toll-like receptors (TLRs) in pregnant heifers immunized with different vaccine formulations against N. caninum before mating and then challenged the heifers with live N. caninum on day 70 of gestation. TLR7 and TLR8 expression was upregulated in the placental caruncle of infected-pregnant heifers previously exposed to live N. caninum as immunogen. However, TLR7 and 8 expression in both placenta and caruncle as well as, TLR3 and 9 expression in caruncle were upregulated when heifers were previously immunized with inactivated soluble whole antigens and recombinant NcSAG1, NcHSP20 and NcGRA7 proteins. All dams were carrying viable fetuses when they were culled at day 104 of gestation. Upregulation of TLR7 and IFNγ expression was detected in fetal spleen when their mothers where previously vaccinated with soluble antigens and recombinant NcSAG1, NcHSP20 and NcGRA7 proteins. These studies demonstrate that soluble or recombinant NcSAG1, NcHSP20 and NcGRA7 antigens induce key TLRs expression at the maternal-fetal interface, probably triggering damaging inflammatory cellular immune responses associated with abortion. Previous infection with N. caninum seems to attenuate the innate immune response at the maternal-fetal interface, which could favour pregnancy maintenance and perpetuation of the disease. This finding represents novel information on how N. caninum vaccination and infection modulate TLRs expression at the placenta and fetal spleen, the possible role in the pregnancy outcomes and transplacental transmission of the protozoa. © 2017 Elsevier B.V.

Registro:

Documento: Artículo
Título:Immunization with inactivated antigens of Neospora caninum induces toll-like receptors 3, 7, 8 and 9 in maternal-fetal interface of infected pregnant heifers
Autor:Marin, M.S.; Hecker, Y.P.; Quintana, S.; Pérez, S.E.; Leunda, M.R.; Cantón, G.J.; Cobo, E.R.; Moore, D.P.; Odeón, A.C.
Filiación:Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia 1917, Buenos Aires, C1033AAJ, Argentina
Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria Balcarce, Ruta 226 Km 73.5, Balcarce, Buenos Aires, 7620, Argentina
Centro de Investigación en Abejas Sociales, Departamento de Biología, Facultad de Ciencias Exactas y Naturales, UNMDP, Funes 3350, Mar del Plata, Buenos Aires, 7600, Argentina
Centro de Investigación Veterinaria de Tandil (CIVETAN, CONICET-CICPBA), Facultad de Ciencias Veterinarias, UNCPBA, Paraje Arroyo Seco s/n, Tandil, Buenos Aires, 7000, Argentina
Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Canada
Palabras clave:Cattle; Maternal-fetal interface; Neospora caninum; Toll-like receptor expression; Vaccine; gamma interferon; heat shock protein 20; inactivated vaccine; parasite antigen; protein GRA7; protein SAG1; toll like receptor 3; toll like receptor 7; toll like receptor 8; toll like receptor 9; unclassified drug; parasite antigen; toll like receptor; abortion; animal experiment; animal model; animal tissue; Article; breeding; cellular immunity; controlled study; dam (animal); female; fetus; heifer; immunity; inflammation; innate immunity; maternal fetal interface; neosporosis; nonhuman; protein expression; spleen; upregulation; vaccination; animal; bovine; cattle disease; coccidiosis; immunization; immunology; metabolism; Neospora; parasitology; placenta; pregnancy; pregnancy complication; veterinary; Animals; Antigens, Protozoan; Cattle; Cattle Diseases; Coccidiosis; Female; Immunity, Cellular; Immunization; Neospora; Placenta; Pregnancy; Pregnancy Complications, Parasitic; Toll-Like Receptors
Año:2017
Volumen:243
Página de inicio:12
Página de fin:17
DOI: http://dx.doi.org/10.1016/j.vetpar.2017.06.005
Handle:http://hdl.handle.net/20.500.12110/paper_03044017_v243_n_p12_Marin
Título revista:Veterinary Parasitology
Título revista abreviado:Vet. Parasitol.
ISSN:03044017
CODEN:VPARD
CAS:gamma interferon, 82115-62-6; toll like receptor 9, 352486-49-8, 390883-32-6; toll like receptor, 409141-78-2; Antigens, Protozoan; Toll-Like Receptors
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044017_v243_n_p12_Marin

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Citas:

---------- APA ----------
Marin, M.S., Hecker, Y.P., Quintana, S., Pérez, S.E., Leunda, M.R., Cantón, G.J., Cobo, E.R.,..., Odeón, A.C. (2017) . Immunization with inactivated antigens of Neospora caninum induces toll-like receptors 3, 7, 8 and 9 in maternal-fetal interface of infected pregnant heifers. Veterinary Parasitology, 243, 12-17.
http://dx.doi.org/10.1016/j.vetpar.2017.06.005
---------- CHICAGO ----------
Marin, M.S., Hecker, Y.P., Quintana, S., Pérez, S.E., Leunda, M.R., Cantón, G.J., et al. "Immunization with inactivated antigens of Neospora caninum induces toll-like receptors 3, 7, 8 and 9 in maternal-fetal interface of infected pregnant heifers" . Veterinary Parasitology 243 (2017) : 12-17.
http://dx.doi.org/10.1016/j.vetpar.2017.06.005
---------- MLA ----------
Marin, M.S., Hecker, Y.P., Quintana, S., Pérez, S.E., Leunda, M.R., Cantón, G.J., et al. "Immunization with inactivated antigens of Neospora caninum induces toll-like receptors 3, 7, 8 and 9 in maternal-fetal interface of infected pregnant heifers" . Veterinary Parasitology, vol. 243, 2017, pp. 12-17.
http://dx.doi.org/10.1016/j.vetpar.2017.06.005
---------- VANCOUVER ----------
Marin, M.S., Hecker, Y.P., Quintana, S., Pérez, S.E., Leunda, M.R., Cantón, G.J., et al. Immunization with inactivated antigens of Neospora caninum induces toll-like receptors 3, 7, 8 and 9 in maternal-fetal interface of infected pregnant heifers. Vet. Parasitol. 2017;243:12-17.
http://dx.doi.org/10.1016/j.vetpar.2017.06.005