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Abstract:

The Laguna Brava Nature Reserve is a stressful habitat in the Andean Mountains (Argentina) dominated by extreme abiotic factors: high altitude and UV radiance, hypersalinity, alkalinity, and high concentrations of toxic elements in the soil. The sparse native vegetation that inhabits Laguna Brava and Mulas Muertas wetlands is frequently colonized by arbuscular mycorrhizal (AM) fungi. It is, however, unknown which AM species can survive in such a harsh environment and how those environmental conditions influence the AM communities. To answer these questions, 454-amplicon pyrosequencing and morphological (based on spore traits) approaches were used to assess fungal diversity. A total of 23 molecular operational taxonomic units and 14 distinct morphospecies of AM fungi were identified. The morphological characterization of AM fungal communities in Laguna Brava and Mulas Muertas, supported by the molecular data, revealed that Glomeraceae and Claroideoglomeraceae were the dominant families, confirming the predominance of generalist and ruderal AM fungal taxa but with stress-tolerant life history traits. Our results showed that the presence of AM fungi is strongly associated with local environmental variations in Laguna Brava (hypersalinity and high Na+, Sr, As and U contents in soils). The AM fungal communities in Laguna Brava and Mulas Muertas wetlands were similar according to the Simpson diversity index and the ecological distance estimated by Bray Curtis index. These results were also supported by the environmental parameters measured, as they did not vary between the studied sites. This study represents the first characterization of AM fungal community in a high-altitude Andean wetland in Argentina, improving our knowledge about these fungi from extreme environments. © 2016, Springer Science+Business Media Dordrecht.

Registro:

Documento: Artículo
Título:Arbuscular mycorrhizal fungal diversity in high-altitude hypersaline Andean wetlands studied by 454-sequencing and morphological approaches
Autor:Silvani, V.A.; Colombo, R.P.; Scorza, M.V.; Fernández Bidondo, L.; Rothen, C.P.; Scotti, A.; Fracchia, S.; Godeas, A.
Filiación:Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, IBBEA-CONICET, Universidad de Buenos Aires, Ciudad Universitaria, 4to piso Pabellón 2, Buenos Aires, 1428, Argentina
International Center for Earth Sciences, Regional Mendoza, Comisión Nacional de Energía Atómica, Padre Contreras 1300, Mendoza, 5500, Argentina
Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja, CRILAR- CONICET, 5301, Entre Ríos y Mendoza, Anillaco, La Rioja, Argentina
Palabras clave:Arbuscular mycorrhizal fungi; Extreme environment; Heavy metal; High-altitude wetlands; Pyrosequencing; Salinity; altitude; arbuscular mycorrhiza; diversity index; extremophile; fungus; genetic analysis; heavy metal; hypersaline environment; life history trait; wetland; Andes; Argentina; Buenos Aires [Argentina]; La Brava Lake; Fungi; Glomeraceae
Año:2017
Volumen:72
Número:2
Página de inicio:143
Página de fin:152
DOI: http://dx.doi.org/10.1007/s13199-016-0454-3
Título revista:Symbiosis
Título revista abreviado:Symbiosis
ISSN:03345114
CODEN:SYMBE
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_03345114_v72_n2_p143_Silvani

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Citas:

---------- APA ----------
Silvani, V.A., Colombo, R.P., Scorza, M.V., Fernández Bidondo, L., Rothen, C.P., Scotti, A., Fracchia, S.,..., Godeas, A. (2017) . Arbuscular mycorrhizal fungal diversity in high-altitude hypersaline Andean wetlands studied by 454-sequencing and morphological approaches. Symbiosis, 72(2), 143-152.
http://dx.doi.org/10.1007/s13199-016-0454-3
---------- CHICAGO ----------
Silvani, V.A., Colombo, R.P., Scorza, M.V., Fernández Bidondo, L., Rothen, C.P., Scotti, A., et al. "Arbuscular mycorrhizal fungal diversity in high-altitude hypersaline Andean wetlands studied by 454-sequencing and morphological approaches" . Symbiosis 72, no. 2 (2017) : 143-152.
http://dx.doi.org/10.1007/s13199-016-0454-3
---------- MLA ----------
Silvani, V.A., Colombo, R.P., Scorza, M.V., Fernández Bidondo, L., Rothen, C.P., Scotti, A., et al. "Arbuscular mycorrhizal fungal diversity in high-altitude hypersaline Andean wetlands studied by 454-sequencing and morphological approaches" . Symbiosis, vol. 72, no. 2, 2017, pp. 143-152.
http://dx.doi.org/10.1007/s13199-016-0454-3
---------- VANCOUVER ----------
Silvani, V.A., Colombo, R.P., Scorza, M.V., Fernández Bidondo, L., Rothen, C.P., Scotti, A., et al. Arbuscular mycorrhizal fungal diversity in high-altitude hypersaline Andean wetlands studied by 454-sequencing and morphological approaches. Symbiosis. 2017;72(2):143-152.
http://dx.doi.org/10.1007/s13199-016-0454-3