Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor


We characterized molecularly the first freshwater member ever reported for the family Bathycoccaceae in Lake Musters (Argentinean Patagonia). Members of this family are extremely numerous and play a key ecological role in marine systems as primary producers. We cloned a fragment comprising the SSU rRNA gene + ITS region from environmental DNA using specific mamiellophyte primers. The unique SSU rRNA gene sequence obtained clustered robustly with Bathycoccus prasinos. Analysis of the two-dimensional structure of the ITS region showed the presence of a typical supplementary helix in the ITS-2 region, a synapomorphy of Bathycoccaceae, which confirmed further its phylogenetic placement. We finally discuss the possible causes for the presence of this organism in Lake Musters. © 2017 Elsevier GmbH


Documento: Artículo
Título:First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey
Autor:Lara, E.; Fernández, L.D.; Schiaffino, M.R.; Izaguirre, I.
Filiación:Laboratory of Soil Biology, University of Neuchâtel, Rue Emile Argand 11, Neuchâtel, CH-2000, Switzerland
Centro de Investigación en Recursos Naturales y Sustentabilidad (CIRENYS), Universidad Bernardo O'Higgins, Fábrica 1990, Santiago, Chile
Centro de Investigaciones y transferencia del Noroeste de la Provincia de Buenos Aires (CITNOBA, CONICET-UNNOBA), Junín, 6000, Argentina
Departamento de Ecología, Genética y Evolución, IEGEBA (UBA-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Real Jardín Botánico, CSIC, Plaza de Murillo 2, Madrid, E-28014, Spain
Palabras clave:Mamiellophyceae; Picoplankton; Ribosomal genes; Southern Patagonia; ribosomal spacer DNA; ribosome DNA; chemistry; classification; genetics; green alga; lake; metagenomics; phylogeny; species difference; Chlorophyta; DNA, Ribosomal; DNA, Ribosomal Spacer; Lakes; Metagenomics; Phylogeny; Species Specificity
Página de inicio:45
Página de fin:49
Título revista:European Journal of Protistology
Título revista abreviado:Eur. J. Protistol.
CAS:DNA, Ribosomal; DNA, Ribosomal Spacer


  • Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J.H., Zhang, Z., Miller, W., Lipman, D.J., Gapped BLAST and PSI-BLAST: a new generation of protein database search programs (1997) Nucleic Acids Res., 25, pp. 3389-3402
  • Balzano, S., Marie, D., Gourvil, P., Vaulot, D., Composition of the summer photosynthetic pico and nanoplankton communities in the Beaufort Sea assessed by T-RFLP and sequences of the 18S rRNA gene from flow cytometry sorted samples (2012) ISME J., 6, pp. 1480-1498
  • Charvet, S., Vincent, W.F., Lovejoy, C., Chrysophytes and other protists in High Arctic lakes: molecular gene surveys, pigment signatures and microscopy (2012) Polar Biol., 35, pp. 733-748
  • Collado-Fabbri, S., Vaulot, D., Ulloa, O., Structure and seasonal dynamics of the eukaryotic picophytoplankton community in a wind-driven coastal upwelling ecosystem (2011) Limnol. Oceanogr., 56, pp. 2334-2346
  • Countway, P.D., Caron, D.A., Abundance and distribution of Ostreococcus sp. in the San Pedro Channel California, as revealed by quantitative PCR (2006) Appl. Environ. Microbiol., 72, pp. 2496-2506
  • Derelle, E., Ferraz, C., Rombauts, S., Rouze, P., Worden, A.Z., Robbens, S., Partensky, F., Moreau, H., Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features (2006) Proc. Natl. Acad. Sci. U. S. A., 103, pp. 11647-11652
  • Diez, B., Pedros-Alio, C., Marsh, T.L., Massana, R., Application of denaturing gradient gel electrophoresis (DGGE) to study the diversity of marine picoeukaryotic assemblages and comparison of DGGE with other molecular techniques (2001) Appl. Environ. Microbiol., 67, pp. 2942-2951
  • Diez, B., Pedros-Alio, C., Massana, R., Study of genetic diversity of eukaryotic picoplankton in different oceanic regions by small-subunit rRNA gene cloning and sequencing (2001) Appl. Environ. Microbiol., 67, pp. 2932-2941
  • Fernández, L.D., Lara, E., Mitchell, E.A.D., Checklist diversity and distribution of testate amoebae in Chile (2015) Eur. J. Protistol., 51, pp. 409-424
  • Fuller, N.J., Campbell, C., Allen, D.J., Pitt, F.D., Zwirglmaierl, K., Le Gall, F., Analysis of photosynthetic picoeukaryote diversity at open ocean sites in the Arabian Sea using a PCR biased towards marine algal plastids (2006) Aquat. Microb. Ecol., 43, pp. 79-93
  • Guillou, L., Eikrem, W., Chretiennot-Dinet, M.J., Le Gall, F., Massana, R., Romari, K., Diversity of picoplanktonic prasinophytes assessed by direct nuclear SSU rDNA sequencing of environmental samples and novel isolates retrieved from oceanic and coastal marine ecosystems (2004) Protist, 155, pp. 193-214
  • Kulk, G., van de Poll, W.H., Visser, R.J.W., Buma, A.G.J., Distinct differences in photoacclimation potential between prokaryotic and eukaryotic oceanic phytoplankton (2011) J. Exp. Mar. Biol. Ecol., 398, pp. 63-72
  • Lefranc, M., Thenot, A., Lepere, U., Debroas, D., Genetic diversity of small eukaryotes in lakes differing by their trophic status (2005) Appl. Environ. Microbiol., 71, pp. 5935-5942
  • Lepere, C., Masquelier, S., Mangot, J.F., Debroas, D., Domaizon, I., Vertical structure of small eukaryotes in three lakes that differ by their trophic status: a quantitative approach (2010) ISME J., 4, pp. 1509-1519
  • Li, W.K.W., Primary production of prochlorophytes, cyanobacteria, and eukaryotic ultraphytoplankton: measurements from flow cytometric sorting (1994) Limnol. Oceanogr., 39, pp. 169-175
  • Mangot, J.F., Domaizon, I., Taib, N., Marouni, N., Duffaud, E., Bronner, G., Debroas, D., Short-term dynamics of diversity patterns: evidence of continual reassembly within lacustrine small eukaryotes (2013) Environ. Microbiol., 15, pp. 1745-1758
  • Marin, B., Melkonian, M., Molecular phylogeny and classification of the Mamiellophyceae class. nov. (Chlorophyta) based on sequence comparisons of the nuclear- and plastid-encoded rRNA operons (2010) Protist, 161, pp. 304-336
  • Moon-van der Staay, S.Y., De Wachter, R., Vaulot, D., Oceanic 18S rDNA sequences from picoplankton reveal unsuspected eukaryotic diversity (2001) Nature, 409, pp. 607-610
  • Newbold, L.K., Oliver, A.E., Booth, T., Tiwari, B., DeSantis, T., Maguire, M., Andersen, G., Whiteley, A.S., The response of marine picoplankton to ocean acidification (2012) Environ. Microbiol., 14, pp. 2293-2307
  • Not, F., Latasa, M., Marie, D., Cariou, T., Vaulot, D., Simon, N., A single species, Micromonas pusilla (Prasinophyceae), dominates the eukaryotic picoplankton in the western English channel (2004) Appl. Environ. Microbiol., 70, pp. 4064-4072
  • Not, F., Siano, R., Kooistra, W., Simon, N., Vaulot, D., Probert, I., Diversity and ecology of eukaryotic marine phytoplankton (2012) Genomic Insights into the Biology of Algae, pp. 1-53. , G. Piganeau Academic Press Ltd.—Elsevier Science Ltd. London
  • Nullo, F., Combina, A., Patagonian continental deposits (Cretaceous–Tertiary) (2011) Biol. J. Linnean Soc., 103, pp. 289-304
  • Richards, T.A., Vepritskiy, A.A., Gouliamova, D.E., Nierzwicki-Bauer, S.A., The molecular diversity of freshwater picoeukaryotes from an oligotrophic lake reveals diverse, distinctive and globally dispersed lineages (2005) Environ. Microbiol., 7, pp. 1413-1425
  • Schiaffino, M.R., Lara, E., Fernández, L.D., Balagué, V., Singer, D., Seppey, C.C.W., Massana, R., Izaguirre, I., Microbial eukaryote communities exhibit robust biogeographical patterns along a gradient of Patagonian and Antarctic lakes (2016) Environ. Microbiol., 18, pp. 5249-5264
  • Slapeta, J., Moreira, D., Lopez-Garcia, P., The extent of protist diversity: insights from molecular ecology of freshwater eukaryotes (2005) Proc. R. Soc. B: Biol. Sci., 272, pp. 2073-2081
  • Stamatakis, A., Hoover, P., Rougemont, J., A rapid bootstrap algorithm for the RAxML web servers (2008) Syst. Biol., 57, pp. 758-771
  • Subirana, L., Pequin, B., Michely, S., Escande, M.L., Meilland, J., Derelle, E., Marin, B., Grimsley, N.H., Morphology, genome plasticity, and phylogeny in the genus Ostreococcus reveal a cryptic species O. mediterraneus sp. nov. (Mamiellales, Mamiellophyceae) (2013) Protist, 164, pp. 643-659
  • Taib, N., Mangot, J.F., Domaizon, I., Bronner, G., Debroas, D., Phylogenetic affiliation of SSU rRNA genes generated by massively parallel sequencing: new insights into the freshwater protist diversity (2013) PLoS One, 8
  • Thompson, J.D., Higgins, D.G., Gibson, T.J., CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice (1994) Nucleic Acids Res., 22, pp. 4673-4680
  • Woelfl, S., Notas sobre protozoos ciliados dulceacuicolas de Chile (2006) Gayana, 70, pp. 24-26
  • Worden, A.Z., Picoeukaryote diversity in coastal waters of the Pacific Ocean (2006) Aquat. Microb. Ecol., 43, pp. 165-175
  • Wu, Q.L.L., Chatzinotas, A., Wang, J.J., Boenigk, J., Genetic diversity of eukaryotic plankton assemblages in Eastern Tibetan Lakes differing by their salinity and altitude (2009) Microb. Ecol., 58, pp. 569-581
  • Zhao, B.Y., Chen, M.J., Sun, Y., Yang, J.X., Chen, F.Z., Genetic diversity of picoeukaryotes in eight lakes differing in trophic status (2011) Can. J. Microbiol., 57, pp. 115-126
  • Zuker, M., Mfold web server for nucleic acid folding and hybridization prediction (2003) Nucleic Acids Res., 31, pp. 3406-3415


---------- APA ----------
Lara, E., Fernández, L.D., Schiaffino, M.R. & Izaguirre, I. (2017) . First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey. European Journal of Protistology, 60, 45-49.
---------- CHICAGO ----------
Lara, E., Fernández, L.D., Schiaffino, M.R., Izaguirre, I. "First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey" . European Journal of Protistology 60 (2017) : 45-49.
---------- MLA ----------
Lara, E., Fernández, L.D., Schiaffino, M.R., Izaguirre, I. "First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey" . European Journal of Protistology, vol. 60, 2017, pp. 45-49.
---------- VANCOUVER ----------
Lara, E., Fernández, L.D., Schiaffino, M.R., Izaguirre, I. First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey. Eur. J. Protistol. 2017;60:45-49.