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Abstract:

We characterized molecularly the first freshwater member ever reported for the family Bathycoccaceae in Lake Musters (Argentinean Patagonia). Members of this family are extremely numerous and play a key ecological role in marine systems as primary producers. We cloned a fragment comprising the SSU rRNA gene + ITS region from environmental DNA using specific mamiellophyte primers. The unique SSU rRNA gene sequence obtained clustered robustly with Bathycoccus prasinos. Analysis of the two-dimensional structure of the ITS region showed the presence of a typical supplementary helix in the ITS-2 region, a synapomorphy of Bathycoccaceae, which confirmed further its phylogenetic placement. We finally discuss the possible causes for the presence of this organism in Lake Musters. © 2017 Elsevier GmbH

Registro:

Documento: Artículo
Título:First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey
Autor:Lara, E.; Fernández, L.D.; Schiaffino, M.R.; Izaguirre, I.
Filiación:Laboratory of Soil Biology, University of Neuchâtel, Rue Emile Argand 11, Neuchâtel, CH-2000, Switzerland
Centro de Investigación en Recursos Naturales y Sustentabilidad (CIRENYS), Universidad Bernardo O'Higgins, Fábrica 1990, Santiago, Chile
Centro de Investigaciones y transferencia del Noroeste de la Provincia de Buenos Aires (CITNOBA, CONICET-UNNOBA), Junín, 6000, Argentina
Departamento de Ecología, Genética y Evolución, IEGEBA (UBA-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Real Jardín Botánico, CSIC, Plaza de Murillo 2, Madrid, E-28014, Spain
Palabras clave:Mamiellophyceae; Picoplankton; Ribosomal genes; Southern Patagonia; ribosomal spacer DNA; ribosome DNA; chemistry; classification; genetics; green alga; lake; metagenomics; phylogeny; species difference; Chlorophyta; DNA, Ribosomal; DNA, Ribosomal Spacer; Lakes; Metagenomics; Phylogeny; Species Specificity
Año:2017
Volumen:60
Página de inicio:45
Página de fin:49
DOI: http://dx.doi.org/10.1016/j.ejop.2017.05.008
Título revista:European Journal of Protistology
Título revista abreviado:Eur. J. Protistol.
ISSN:09324739
CODEN:EJPRE
CAS:DNA, Ribosomal; DNA, Ribosomal Spacer
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_09324739_v60_n_p45_Lara

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Citas:

---------- APA ----------
Lara, E., Fernández, L.D., Schiaffino, M.R. & Izaguirre, I. (2017) . First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey. European Journal of Protistology, 60, 45-49.
http://dx.doi.org/10.1016/j.ejop.2017.05.008
---------- CHICAGO ----------
Lara, E., Fernández, L.D., Schiaffino, M.R., Izaguirre, I. "First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey" . European Journal of Protistology 60 (2017) : 45-49.
http://dx.doi.org/10.1016/j.ejop.2017.05.008
---------- MLA ----------
Lara, E., Fernández, L.D., Schiaffino, M.R., Izaguirre, I. "First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey" . European Journal of Protistology, vol. 60, 2017, pp. 45-49.
http://dx.doi.org/10.1016/j.ejop.2017.05.008
---------- VANCOUVER ----------
Lara, E., Fernández, L.D., Schiaffino, M.R., Izaguirre, I. First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey. Eur. J. Protistol. 2017;60:45-49.
http://dx.doi.org/10.1016/j.ejop.2017.05.008