Artículo

Conforte, V.P.; Yaryura, P.M.; Bianco, M.I.; Rodríguez, M.C.; Daglio, Y.; Prieto, E.; Schilardi, P.; Vojnov, A.A."Changes in the physico-chemical properties of the xanthan produced by Xanthomonas citri subsp. citri in grapefruit leaf extract" (2019) Glycobiology. 29(3):269-278
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Abstract:

Xanthan is a virulence factor produced by Xanthomonas spp. We previously demonstrated that this exopolysaccharide is not only essential for pathogenicity by contributing with bacterial survival but also its pyruvate substituents interfere with some plant defense responses. Deepening our studies about xanthan properties and structure, the aim of this work was to analyze the characteristics of xanthan produced by Xanthomonas in different culture media. We analyzed the xanthan produced by Xanthomonas citri subsp. citri (Xcc) in leaf extracts from grapefruit (a susceptible host of this bacterium) and compared it with the xanthan produced in a synthetic culture medium. We found that the xanthan produced in the grapefruit extract (Xan-GLE) presented shorter and more disordered molecules than xanthan produced in the synthetic medium (Xan-PYM). Besides, Xan-GLE resulted less viscous than Xan-PYM. The disordered molecular conformation of Xan-GLE could be attributed to its higher pyruvilation degree and lower acetylation degree compared with those detected in Xan-PYM. Meanwhile, the difference in the viscosity of both xanthans could be due to their molecules length. Finally, we cultured Xcc in the presence of the Xan-GLE or Xan-PYM and observed the formation of biofilm-like structures in both cases. We found significant differences in biofilm architecture between the two conditions, being the biofilm produced in presence of Xan-GLE similar to that formed in canker lesions developed in lemon plant leaves. Together, these results show how xanthan structure and properties changed when Xcc grew in a natural substrate and can contribute to better understand the biological role of xanthan. © © The Author(s) 2019. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Registro:

Documento: Artículo
Título:Changes in the physico-chemical properties of the xanthan produced by Xanthomonas citri subsp. citri in grapefruit leaf extract
Autor:Conforte, V.P.; Yaryura, P.M.; Bianco, M.I.; Rodríguez, M.C.; Daglio, Y.; Prieto, E.; Schilardi, P.; Vojnov, A.A.
Filiación:Instituto de Ciencia y Tecnología Dr. César Milstein, Fundación Pablo Cassará, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Saladillo, Ciudad de Buenos Aires, 2468 (C1440FFX), Argentina
Centro de Investigaciones y Transferencia (CIT Villa María), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Ciencias Básicas y Aplicadas, Universidad Nacional de Villa María., Av. Arturo Jauretche 1555, (5900), Villa María, Córdoba, Argentina
Niversidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Biodiversidad y Biología Experimental, Ciudad Universitaria-Pabellón 2, C1428EGA., Ciudad Autónoma de Buenos Aires, Argentina
Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Centro de Investigación en Hidratos de Carbono (CIHIDECAR), Facultad de Ciencias Exactas y Naturales Pabellón II, 3er P, Ciudad Universitaria, Buenos Aires, 1428, Argentina
Universidad Nacional de la Plata (UNLP), Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas, Consejo Nacional de Investigaciones Científicas y Tecnológicas INIFTA - CONICET), Diagonal 113 y 64 S/N La Plata, Buenos Aires, Argentina
Palabras clave:grapefruit leaf extract; xanthan; Xanthomonas citri subsp. citri
Año:2019
Volumen:29
Número:3
Página de inicio:269
Página de fin:278
DOI: http://dx.doi.org/10.1093/glycob/cwy114
Handle:http://hdl.handle.net/20.500.12110/paper_09596658_v29_n3_p269_Conforte
Título revista:Glycobiology
Título revista abreviado:Glycobiology
ISSN:09596658
CODEN:GLYCE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09596658_v29_n3_p269_Conforte

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Citas:

---------- APA ----------
Conforte, V.P., Yaryura, P.M., Bianco, M.I., Rodríguez, M.C., Daglio, Y., Prieto, E., Schilardi, P.,..., Vojnov, A.A. (2019) . Changes in the physico-chemical properties of the xanthan produced by Xanthomonas citri subsp. citri in grapefruit leaf extract. Glycobiology, 29(3), 269-278.
http://dx.doi.org/10.1093/glycob/cwy114
---------- CHICAGO ----------
Conforte, V.P., Yaryura, P.M., Bianco, M.I., Rodríguez, M.C., Daglio, Y., Prieto, E., et al. "Changes in the physico-chemical properties of the xanthan produced by Xanthomonas citri subsp. citri in grapefruit leaf extract" . Glycobiology 29, no. 3 (2019) : 269-278.
http://dx.doi.org/10.1093/glycob/cwy114
---------- MLA ----------
Conforte, V.P., Yaryura, P.M., Bianco, M.I., Rodríguez, M.C., Daglio, Y., Prieto, E., et al. "Changes in the physico-chemical properties of the xanthan produced by Xanthomonas citri subsp. citri in grapefruit leaf extract" . Glycobiology, vol. 29, no. 3, 2019, pp. 269-278.
http://dx.doi.org/10.1093/glycob/cwy114
---------- VANCOUVER ----------
Conforte, V.P., Yaryura, P.M., Bianco, M.I., Rodríguez, M.C., Daglio, Y., Prieto, E., et al. Changes in the physico-chemical properties of the xanthan produced by Xanthomonas citri subsp. citri in grapefruit leaf extract. Glycobiology. 2019;29(3):269-278.
http://dx.doi.org/10.1093/glycob/cwy114