Artículo

Ferreira, M.L.; Casabuono, A.C.; Stacchiotti, S.T.; Couto, A.S.; Ramirez, S.A.; Vullo, D.L. "Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes" (2017) International Biodeterioration and Biodegradation. 119:605-613
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Abstract:

The production, isolation, purification and characterization of soluble Extracellular Polymeric Substances (EPS) secreted by Pseudomonas veronii 2E were studied. Optimal production was obtained in medium M9-14 g l−1 glycerol, 25 °C. Spectrophotometric and potentiometric titration studies showed that EPS consisted on extracellular DNA, proteins and polysaccharides as the main components. DNase I, Proteinase K and further sodium deoxycholate treatments yielded similar proportions of lipopolysaccharides (LPSs) and exopolysaccharides (ExPs). ExP was subjected to total acid hydrolysis and then analyzed by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD), showing the presence of fucose, galactosamine, glucosamine, galactose, glucose, mannose and glucuronic acid. Further treatment with oxalic acid confirmed the presence of a pyruvilated sugar. The ability of the secreted products to interact with Cd(II) was studied by anodic stripping voltammetry (ASV). LPS fraction contained the highest ligand concentration (279 ± 10 μmoles Cd(II) binding sites g LPS−1). This information is a valuable tool for the application of EPS in industrial wastewater treatment. © 2016 Elsevier Ltd

Registro:

Documento: Artículo
Título:Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes
Autor:Ferreira, M.L.; Casabuono, A.C.; Stacchiotti, S.T.; Couto, A.S.; Ramirez, S.A.; Vullo, D.L.
Filiación:Área Química, Instituto de Ciencias, Universidad Nacional de General Sarmiento, J.M. Gutierrez 1150, Los Polvorines, Buenos Aires B1613GSX, Argentina
Consejo Nacional de lnvestigaciones Científicas y Técnicas, Av. Rivadavia 1917, Buenos Aires, C1033AAJ, Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Orgánica - Consejo Nacional de lnvestigaciones Cientificas y Técnicas, Centro de Investigación en Hidratos de Carbono (CIHIDECAR), Buenos Aires, Argentina
Palabras clave:Bacterial lipopolysaccharide (LPS); Biotreatment; Cadmium retention; Extracellular polymeric substances (EPS); Pseudomonas veronii 2E; Bacteria; Binding sites; Chromatography; Glucose; Industrial water treatment; Ligands; Oxalic acid; Polymers; Polysaccharides; Titration; Voltammetry; Anodic stripping voltammetry; Biotreatments; Extracellular polymeric substances; High-performance anion-exchange chromatography; Industrial wastewater treatment; Lipopolysaccharides; Pseudomonas veronii; Pulsed amperometric detection; Wastewater treatment; bacterium; bioactivity; cadmium; chemical analysis; concentration (composition); exopolymer; hydrolysis; ligand; oxalic acid; polysaccharide; purification; waste treatment; wastewater treatment; Bacteria (microorganisms); Pseudomonas veronii
Año:2017
Volumen:119
Página de inicio:605
Página de fin:613
DOI: http://dx.doi.org/10.1016/j.ibiod.2016.10.013
Título revista:International Biodeterioration and Biodegradation
Título revista abreviado:Int. Biodeterior. Biodegrad.
ISSN:09648305
CODEN:IBBIE
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_09648305_v119_n_p605_Ferreira

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Citas:

---------- APA ----------
Ferreira, M.L., Casabuono, A.C., Stacchiotti, S.T., Couto, A.S., Ramirez, S.A. & Vullo, D.L. (2017) . Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes. International Biodeterioration and Biodegradation, 119, 605-613.
http://dx.doi.org/10.1016/j.ibiod.2016.10.013
---------- CHICAGO ----------
Ferreira, M.L., Casabuono, A.C., Stacchiotti, S.T., Couto, A.S., Ramirez, S.A., Vullo, D.L. "Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes" . International Biodeterioration and Biodegradation 119 (2017) : 605-613.
http://dx.doi.org/10.1016/j.ibiod.2016.10.013
---------- MLA ----------
Ferreira, M.L., Casabuono, A.C., Stacchiotti, S.T., Couto, A.S., Ramirez, S.A., Vullo, D.L. "Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes" . International Biodeterioration and Biodegradation, vol. 119, 2017, pp. 605-613.
http://dx.doi.org/10.1016/j.ibiod.2016.10.013
---------- VANCOUVER ----------
Ferreira, M.L., Casabuono, A.C., Stacchiotti, S.T., Couto, A.S., Ramirez, S.A., Vullo, D.L. Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes. Int. Biodeterior. Biodegrad. 2017;119:605-613.
http://dx.doi.org/10.1016/j.ibiod.2016.10.013