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Abstract:

Bacillus amyloliquefaciens PGPBacCA1 was studied regarding its aptitude to protect common bean seeds from their intrinsic pathogens. Also, the inhibition of different environmental phytopathogenic fungi was tested. Two cultivars of Phaseolus vulgaris L. were evaluated: cv. Nag (black bean) and cv. Alubia (white bean). Aspergillus spp., Penicillium spp. and Fusarium spp. constituted the natural fungal biota of both seeds, whereas white bean and black bean also exhibited Cladosporium spp. and Rhizopus spp., respectively. B. amyloliquefaciens PGPBacCA1 prevented the development of the endophytic fungi of black bean, while only Cladosporium spp. survived in the white variety. Growth chamber assays were carried out and bacilli cells were applied on seeds without affecting neither the vigor nor the germination potential of either type of bean. In addition, B. amyloliquefaciens PGPBacCA1, by dual cultures, was able to inhibit the development of the following phytopathogenic fungi: Sclerotium rolfsii (35%), Sclerotinia sclerotiorum (76.5%), Rhizoctonia solani (73%), Fusarium solani (56.5%), and Penicillium spp. (71.5%). The UV-MALDI TOF MS analysis showed that B. amyloliquefaciens PGPBacCA1 co-produces different homologues of the lipopeptides surfactin, iturin and fengycin in the presence of S. sclerotiorum and F. solani. These compounds were identified as the main responsible for the antagonistic effect. SEM analysis confirmed the antifungal effects of the lipopeptides, which also caused damage to chlamydospores and sclerotia of Fusarium and Sclerotinia, respectively. B. amyloliquefaciens PGPBacCA1 can thus be applied to these bean seeds varieties as a potential bioprotection agent. © 2016 Elsevier Inc.

Registro:

Documento: Artículo
Título:Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens
Autor:Torres, M.J.; Pérez Brandan, C.; Sabaté, D.C.; Petroselli, G.; Erra-Balsells, R.; Audisio, M.C.
Filiación:Instituto de Investigaciones para la Industria Química (INIQUI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Salta, Av. Bolivia 5150, Salta, 4400, Argentina
Instituto Nacional de Tecnología Agropecuaria-Estación Experimental Salta, Ruta Nacional 68 Km 172, Cerrillos, Salta 4403, Argentina
CIHIDECAR-CONICET, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, 3 Ciudad Universitaria, Buenos Aires, 1428, Argentina
Palabras clave:Bacillus amyloliquefaciens; Fusarium solani; Lipopeptides; Phaseolus vulgaris L; Sclerotinia sclerotiorum; Seed-protector
Año:2017
Volumen:105
Página de inicio:93
Página de fin:99
DOI: http://dx.doi.org/10.1016/j.biocontrol.2016.12.001
Título revista:Biological Control
Título revista abreviado:Biol. Control
ISSN:10499644
CODEN:BCIOE
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_10499644_v105_n_p93_Torres

Referencias:

  • Alvarez, F., Castro, M., Príncipe, A., Borioli, G., Fischer, S., Mori, G., Jofré, E., The plant-associated Bacillus amyloliquefaciens strains MEP218 and ARP23 capable of producing the cyclic lipopeptides iturin or surfactin and fengycin are effective in biocontrol of sclerotinia stem rot disease (2011) J Appl. Microbiol., 112, pp. 159-174
  • Aranda, F., Teruel, J., Ortiz, A., Further aspects on the hemolytic activity of the antibiotic lipopeptide iturin A (2005) Biochim. Biophys. Acta, 1713, pp. 51-56
  • Arrebola, E., Jacobs, R., Korsten, L., Iturin A is the principal inhibitor in the biocontrol activity of Bacillus amyloliquefaciens PPCB004 against postharvest fungal pathogens (2010) J. Appl. Microbiol., 108, pp. 386-395
  • Babalola, O., Beneficial bacteria of agricultural importance (2010) Biotechnol. Lett., 32, pp. 1559-1570
  • Barret, M., Briand, M., Bonneau, S., Préveaux, A., Valière, S., Bouchez, O., Hunault, G., Jacquesa, M.A., Emergence shapes the structure of the seed microbiota (2015) Appl. Environ. Microbiol., 81, pp. 1257-1266
  • Basurto-Cadena, M.G., Ambrosio, M.I., García-Jiménez, J., Vázquez-Arista, M., Cambios en la estructura celular durante la actividad antagonica de Bacillus subtilis contra Rhizoctonia solani y Fusarium verticillioides (2010) Acta microscópica, 19, pp. 138-144
  • Berg, G., Plant-microbe interactions promoting plant growth and health: perspectives for controlled use of microorganisms in agriculture (2009) Appl. Microbiol. Biotech., 84, pp. 11-18
  • Bernheimer, A., Avigad, L., Nature and properties of a cytological agent produced by Bacillus subtilis (1970) J. Gen. Microbiol., 61, pp. 361-369
  • Cawoy, H., Debois, D., Franzil, L., De Pauw, E., Thonart, P., Ongena, M., Lipopeptides as main ingredients for inhibition of fungal phytopathogens by Bacillus subtilis/ amyloliquefaciens (2014) Microb Biotechnol., 8, pp. 281-295
  • Choudhary, D., Johri, B., Interactions of Bacillus spp. and plants – with special reference to induced systemic resistance (ISR) (2009) Microbiol. Res., 164, pp. 493-513
  • Chowdhury, S.P., Hartmann, A., Gao, X., Borriss, R., Biocontrol mechanism by root-associated Bacillus amyloliquefaciens FZB42 – a review (2015) Front. Microbiol., 6, p. 780
  • Deleu, M., Paquot, M., Nylander, T., Fengycin interaction with lipid monolayers at the air-aqueous interface – implications for the effect of fengycin on biological membranes (2005) J. Colloid Interface Sci., 283, pp. 358-365
  • Droby, S., Wisniewski, M., Macarisin, D., Wilson, C., Twenty years of postharvest biocontrol research: is it time for new paradigm? (2009) Postharvest Biol. Tec., 52, pp. 137-145
  • Francis, I., Holsters, M., Vereecke, D., The Gram-positive side of plant–microbe interactions (2010) Environ. Microbiol., 12, pp. 1-12
  • García Medina, S., (2002) El cultivo de poroto en la Argentina, , Desimone y Calvo. Gofica Salta, Argentina
  • Grau, A., Gómez Fernández, J.C., Peypoux, F., Ortiz, A., A study on the interactions of surfactin with phospholipid vesicles (1999) Biochim. Biophys. Acta, 1418, pp. 307-319
  • Gray, E.J., Smith, D.L., Intracellular and extracellular PGPR: commonalities and distinctions in the plant–bacterium signaling processes (2005) Soil Biol. Biochem., 37, pp. 395-412
  • Koumoutsi, A., Chen, X.-H., Henne, A., Liesegang, H., Hitzeroth, G., Franke, P., Vater, J., Borriss, R., Structural and functional characterization of gene clusters directing nonribosomal synthesis of bioactive cyclic lipopeptides in Bacillus amyloliquefaciens strain FZB42 (2004) J. Bacteriol., 186, pp. 1084-1096
  • Landa, B., Hervas, A., Bettiol, W., Jiménez-Díaz, R., Antagonistic activity of bacteria from the chickpea rhizosphere against Fusarium oxysporum f. sp. ciceris (1997) Phytoparasitica, 25, pp. 305-318
  • Li, B., Li, Q., Xu, Z., Zhang, N., Shen, Q., Zhang, R., Responses of beneficial Bacillus amyloliquefaciens SQR9 to different soilborne fungal pathogens through the alteration of antifungal compounds production (2014) Front. Microbiol., 5, pp. 636-651
  • Liu, J., Hagberg, I., Novitsky, L., Hadj-Moussa, H., Tyler, J.A., Interaction of antimicrobial cyclic lipopeptides from Bacillus subtilis influences their effect on spore germination and membrane permeability in fungal plant pathogens (2014) Fungal Biol., 118, pp. 855-861
  • Ludueña, L.M., Taurian, T., Tonelli, M.L., Angelini, J.G., Anzuay, M.S., Valetti, L., Munoz, V., Fabra, A., Biocontrol bacterial communities associated with diseased peanut (Arachis hypogaea L.) plants (2012) Eur. J. Soil Biol., 53, pp. 48-55
  • Lugtenberg, B., Kamilova, F., Plant-growth-promoting rhizobacteria (2009) Annu. Rev. Microbiol., 63, pp. 541-556
  • Ongena, M., Jacques, P., Bacillus lipopeptides: versatile weapons for plant disease biocontrol (2008) Trends Microbiol., 16, pp. 116-125
  • Parsa, S., García-Lemos, A.M., Castillo, K., Ortiz, V., Becerra López-Lavalle, L.A., Braun, J., Vega, F.E., Fungal endophytes in germinated seeds of the common bean, Phaseolus vulgaris (2016) Fungal Biol, 120, pp. 783-790
  • Pérez Brandán, C., Caracterización Y Aspectos Biológicos Y Epidemiológicos De La Podredumbre Carbonosa De La Soja (Macrophomina Phaseolina) Para Contribuir a Su Manejo (2009), p. 191. , Tesis de Magister Scientae Universidad de Buenos Aires Buenos Aires, Argentina; Pérez Brandán, C., Altamirano, F., Torres, N., Arzeno, J.L., Corvalán, E., Evaluation of the germination in black bean with phosphate solubilizator rhizobacterias (2005) Biocell, 29, p. 69
  • Pérez-García, A., Romero, D., de Vicente, A., Plant protection and growth stimulation by microorganisms: biotechnological applications of Bacilli in agriculture (2011) Curr. Opin. Biotechnol., 22, pp. 187-193
  • Peypoux, F., Bonmatin, J.M., Wallach, J., Recent trends in the biochemistry of surfactin (1999) Appl. Microbiol. Biotechnol., 51, pp. 553-563
  • Sheppard, J.D., Jumarie, C., Cooper, D.G., Laprade, R., Ionic channels induced by surfactin in planar lipid bilayer membranes (1991) Biochim. Biophys. Acta, 1064, pp. 13-23
  • Souto, G.I., Correa, O.S., Montecchia, M.S., Kerber, N.L., Pucheu, N.L., Bachur, M., Garcia, A.F., Genetic and functional characterization of a Bacillus sp. strain excreting surfactin and antifungal metabolites partially identified as iturin-like compounds (2004) J. Appl. Microbiol., 97, pp. 1247-1256
  • Thimon, L., Peypoux, F., Dana Maget, R., Roux, B., Michel, G., Interactions of bioactive lipopeptides, iturin A and surfactin from Bacillus subtilis (1992) Biotechnol. Appl. Biochem., 16, pp. 144-151
  • Torres, M.J., Pérez Brandán, C., Petroselli, G., Erra-Balsells, R., Audisio, M.C., Antagonistic effects of Bacillus subtilis subsp. subtilis and B. amyloliquefaciens against Macrophomina phaseolina: SEM study of fungal changes and UV-MALDI-TOF MS analysis of their bioactive compounds (2016) Microbiol. Res., 182, pp. 31-39
  • Torres, M.J., Petroselli, G., Daz, M., Erra-Balsells, R., Audisio, M.C., Bacillus subtilis subsp. subtilis CBMDC3f with antimicrobial activity against Gram-positive foodborne pathogenic bacteria: UV-MALDI-TOF MS analysis of its bioactive compounds (2015) World J. Microbiol. Biotechnol., 31, pp. 929-940
  • Wu, L., Wu, H.-J., Qiao, J., Gao, X., Borriss, R., Novel routes for improving biocontrol activity of Bacillus based bioinoculants (2015) Front. Microbiol., 6, p. 1395

Citas:

---------- APA ----------
Torres, M.J., Pérez Brandan, C., Sabaté, D.C., Petroselli, G., Erra-Balsells, R. & Audisio, M.C. (2017) . Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens. Biological Control, 105, 93-99.
http://dx.doi.org/10.1016/j.biocontrol.2016.12.001
---------- CHICAGO ----------
Torres, M.J., Pérez Brandan, C., Sabaté, D.C., Petroselli, G., Erra-Balsells, R., Audisio, M.C. "Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens" . Biological Control 105 (2017) : 93-99.
http://dx.doi.org/10.1016/j.biocontrol.2016.12.001
---------- MLA ----------
Torres, M.J., Pérez Brandan, C., Sabaté, D.C., Petroselli, G., Erra-Balsells, R., Audisio, M.C. "Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens" . Biological Control, vol. 105, 2017, pp. 93-99.
http://dx.doi.org/10.1016/j.biocontrol.2016.12.001
---------- VANCOUVER ----------
Torres, M.J., Pérez Brandan, C., Sabaté, D.C., Petroselli, G., Erra-Balsells, R., Audisio, M.C. Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens. Biol. Control. 2017;105:93-99.
http://dx.doi.org/10.1016/j.biocontrol.2016.12.001