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Abstract:

Proteins are main targets of oxidants in biological systems. This oxidation may occur in the protein backbone as well as in certain amino acid side chains, depending on the oxidant and amino acid intrinsic reactivity. Moreover, many enzymes are capable of generating stable amino acid radicals, such as tyrosyl, tryptophanyl and cysteinyl radicals. These species react very rapidly (many times as diffusion-controlled reactions) with relevant cellular open-shell species such as nitric oxide (·NO) or molecular oxygen (O 2 ). The exception to this apparent rule is tyrosyl radical, that reacts at diffusion rates with ·NO, but shows very slow reactivity towards O 2 (rate constant <10 3 M −1 s −1 ). In this work, we provide a comparative molecular-level description of the reaction mechanisms involved in the reactions of tyrosyl, tryptophanyl and cysteinyl radicals towards ·NO and O 2 , through quantum mechanics simulations which allow us to obtain relevant energetic and structural parameters, proposing a molecular explanation to this tyrosyl discrimination capability, namely, its marginal reactivity with O 2 . © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.

Registro:

Documento: Artículo
Título:A computational investigation of the reactions of tyrosyl, tryptophanyl, and cysteinyl radicals with nitric oxide and molecular oxygen
Autor:Pedron, F.N.; Bartesaghi, S.; Estrin, D.A.; Radi, R.; Zeida, A.
Filiación:Departamento de Química Inorgánica, Analítica y Química-Física and INQUIMAE-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires Ciudad Universitaria, Buenos Aires, Argentina
Departamento de Bioquímica, Universidad de la República, Montevideo, Uruguay
Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
Palabras clave:Amino acid-derived radicals; nitric oxide; one-electron oxidation; oxygen; cysteinyl radical; nitric oxide; oxidizing agent; oxygen; radical; tryptophanyl radical; tyrosyl radical; unclassified drug; Article; diffusion; energy transfer; mathematical computing; oxidation; quantum mechanics; rate constant; reaction analysis
Año:2019
Volumen:53
Número:1
Página de inicio:18
Página de fin:25
DOI: http://dx.doi.org/10.1080/10715762.2018.1541322
Título revista:Free Radical Research
Título revista abreviado:Free Radic. Res.
ISSN:10715762
CODEN:FRARE
CAS:nitric oxide, 10102-43-9; oxygen, 7782-44-7
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_10715762_v53_n1_p18_Pedron

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Citas:

---------- APA ----------
Pedron, F.N., Bartesaghi, S., Estrin, D.A., Radi, R. & Zeida, A. (2019) . A computational investigation of the reactions of tyrosyl, tryptophanyl, and cysteinyl radicals with nitric oxide and molecular oxygen. Free Radical Research, 53(1), 18-25.
http://dx.doi.org/10.1080/10715762.2018.1541322
---------- CHICAGO ----------
Pedron, F.N., Bartesaghi, S., Estrin, D.A., Radi, R., Zeida, A. "A computational investigation of the reactions of tyrosyl, tryptophanyl, and cysteinyl radicals with nitric oxide and molecular oxygen" . Free Radical Research 53, no. 1 (2019) : 18-25.
http://dx.doi.org/10.1080/10715762.2018.1541322
---------- MLA ----------
Pedron, F.N., Bartesaghi, S., Estrin, D.A., Radi, R., Zeida, A. "A computational investigation of the reactions of tyrosyl, tryptophanyl, and cysteinyl radicals with nitric oxide and molecular oxygen" . Free Radical Research, vol. 53, no. 1, 2019, pp. 18-25.
http://dx.doi.org/10.1080/10715762.2018.1541322
---------- VANCOUVER ----------
Pedron, F.N., Bartesaghi, S., Estrin, D.A., Radi, R., Zeida, A. A computational investigation of the reactions of tyrosyl, tryptophanyl, and cysteinyl radicals with nitric oxide and molecular oxygen. Free Radic. Res. 2019;53(1):18-25.
http://dx.doi.org/10.1080/10715762.2018.1541322