Abstract:
Since introduction of sinapinic acid (SA) and α-cyano-4-hydroxycinnamic acid as matrices, successful application of matrix-assisted laser desorption/ionization mass spectrometry started for protein/polypeptides. Both show some limitations in short peptide analysis because matrix clusters are quite abundant. Cinnamics currently used are E-cinnamics. Here, Z-SA as matrix for peptides is studied and compared with E-SA and α-cyano-4-hydroxycinnamic acid. Minor number of clusters is always observed in the low m/z region allowing the detection of short peptides. The results here described show that this novel matrix is a tool of choice for direct, rapid and sensitive detection of hydrophilic and hydrophobic peptides. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.
Registro:
Documento: |
Artículo
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Título: | Application of Z-sinapinic matrix in peptide MALDI-MS analysis |
Autor: | Salum, M.L.; Giudicessi, S.L.; Schmidt De León, T.; Camperi, S.A.; Erra-Balsells, R. |
Filiación: | CIHDECAR-CONICET y Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon II, 3 piso, Ciudad Universitaria, Buenos Aires, 1428, Argentina Consejo Nacional de lnvestigaciones Cientificas y Técnicas, Instituto de Nanobiotecnología (NANOBIOTEC), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires, 1113, Argentina Facultad de Farmacia y Bioquímica, Cátedra de Biotecnología, Universidad de Buenos Aires, Junín 956, Buenos Aires, 1113, Argentina
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Palabras clave: | CHCA clusters; MALDI-MS; peptides; Z-sinapinic acid; alpha cyano 4 hydroxycinnamic acid; angiotensin I; angiotensin II; bradykinin; peptide; sinapic acid; alpha-cyano-4-hydroxycinnamate; cinnamic acid; cinnamic acid derivative; coumaric acid; peptide; sinapic acid; limit of detection; matrix-assisted laser desorption-ionization mass spectrometry; molecular model; Note; peptide analysis; peptide synthesis; priority journal; amino acid sequence; chemical phenomena; chemistry; matrix-assisted laser desorption-ionization mass spectrometry; Amino Acid Sequence; Cinnamates; Coumaric Acids; Hydrophobic and Hydrophilic Interactions; Limit of Detection; Models, Molecular; Peptides; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
Año: | 2017
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Volumen: | 52
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Número: | 3
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Página de inicio: | 182
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Página de fin: | 186
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DOI: |
http://dx.doi.org/10.1002/jms.3908 |
Título revista: | Journal of Mass Spectrometry
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Título revista abreviado: | J. Mass Spectrom.
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ISSN: | 10765174
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CODEN: | JMSPF
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CAS: | alpha cyano 4 hydroxycinnamic acid, 28166-41-8; angiotensin I, 9041-90-1; angiotensin II, 11128-99-7, 68521-88-0; bradykinin, 58-82-2, 5979-11-3; sinapic acid, 530-59-6; cinnamic acid, 4151-45-5, 538-42-1, 621-82-9; coumaric acid, 25429-38-3; alpha-cyano-4-hydroxycinnamate; Cinnamates; cinnamic acid; Coumaric Acids; Peptides; sinapinic acid
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10765174_v52_n3_p182_Salum |
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Citas:
---------- APA ----------
Salum, M.L., Giudicessi, S.L., Schmidt De León, T., Camperi, S.A. & Erra-Balsells, R.
(2017)
. Application of Z-sinapinic matrix in peptide MALDI-MS analysis. Journal of Mass Spectrometry, 52(3), 182-186.
http://dx.doi.org/10.1002/jms.3908---------- CHICAGO ----------
Salum, M.L., Giudicessi, S.L., Schmidt De León, T., Camperi, S.A., Erra-Balsells, R.
"Application of Z-sinapinic matrix in peptide MALDI-MS analysis"
. Journal of Mass Spectrometry 52, no. 3
(2017) : 182-186.
http://dx.doi.org/10.1002/jms.3908---------- MLA ----------
Salum, M.L., Giudicessi, S.L., Schmidt De León, T., Camperi, S.A., Erra-Balsells, R.
"Application of Z-sinapinic matrix in peptide MALDI-MS analysis"
. Journal of Mass Spectrometry, vol. 52, no. 3, 2017, pp. 182-186.
http://dx.doi.org/10.1002/jms.3908---------- VANCOUVER ----------
Salum, M.L., Giudicessi, S.L., Schmidt De León, T., Camperi, S.A., Erra-Balsells, R. Application of Z-sinapinic matrix in peptide MALDI-MS analysis. J. Mass Spectrom. 2017;52(3):182-186.
http://dx.doi.org/10.1002/jms.3908