Astort, F.; Mercau, M.; Giordanino, E.; Degese, M.S.; Caldareri, L.; Coso, O.; Cymeryng, C.B. "Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction" (2014) Nitric Oxide - Biology and Chemistry. 37(1):1-10
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Induction of microsomal heme oxygenase 1 (HO-1) activity is considered a cytoprotective mechanism in different cell types. In adrenal cells, HO-1 induction by ACTH exerts a modulatory effect on steroid production as well. As nitric oxide (NO) has been also regarded as an autocrine/paracrine modulator of adrenal steroidogenesis we sought to study the effects of NO on the induction of HO-1 and the mechanism involved. We hereby analyzed the time and dose-dependent effect of a NO-donor (DETA/NO) on HO-1 induction in a murine adrenocortical cell line. We showed that this effect is mainly exerted at a transcriptional level as it is inhibited by actinomycin D and HO-1 mRNA degradation rates were not affected by DETA/NO treatment. HO-1 induction by NO does not appear to involve the generation of oxidative stress as it was not affected by antioxidant treatment. We also demonstrated that NO-treatment results in the nuclear translocation of the nuclear factor-erythroid 2-related factor (Nrf2), an effect that is attenuated by transfecting the cells with a dominant negative isoform of Nrf2. We finally show that the effects of the NO-donor are reproduced by a permeable analog of cGMP and that a soluble guanylate cyclase specific inhibitor blocked both the induction of HO-1 by NO and the nuclear translocation of Nrf2. © 2013 Elsevier Inc. All rights reserved.


Documento: Artículo
Título:Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction
Autor:Astort, F.; Mercau, M.; Giordanino, E.; Degese, M.S.; Caldareri, L.; Coso, O.; Cymeryng, C.B.
Filiación:Departamento de Bioquímica Humana, Facultad de Medicina, UBA, Paraguay 2155 5, C1121ABG, Argentina
Departamento de Fisiología y Biología Molecular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Idioma: Inglés
Palabras clave:cGMP; Heme oxygenase 1; Nitric oxide; Nuclear factor-erythroid 2-related factor (Nrf2); Soluble guanylate cyclase
Página de inicio:1
Página de fin:10
Título revista:Nitric Oxide - Biology and Chemistry
Título revista abreviado:Nitric Oxide Biol. Chem.


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---------- APA ----------
Astort, F., Mercau, M., Giordanino, E., Degese, M.S., Caldareri, L., Coso, O. & Cymeryng, C.B. (2014) . Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction. Nitric Oxide - Biology and Chemistry, 37(1), 1-10.
---------- CHICAGO ----------
Astort, F., Mercau, M., Giordanino, E., Degese, M.S., Caldareri, L., Coso, O., et al. "Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction" . Nitric Oxide - Biology and Chemistry 37, no. 1 (2014) : 1-10.
---------- MLA ----------
Astort, F., Mercau, M., Giordanino, E., Degese, M.S., Caldareri, L., Coso, O., et al. "Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction" . Nitric Oxide - Biology and Chemistry, vol. 37, no. 1, 2014, pp. 1-10.
---------- VANCOUVER ----------
Astort, F., Mercau, M., Giordanino, E., Degese, M.S., Caldareri, L., Coso, O., et al. Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction. Nitric Oxide Biol. Chem. 2014;37(1):1-10.