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Abstract:

A primary mode-of-action of all pyrethroid insecticides (PYRs) is the disruption of the voltage-gated sodium channel electrophysiology in neurons of target pests and nontarget species. The neurological actions of PYRs on non-neuronal cells of the nervous system remain poorly investigated. In the present work, we used C6 astrocytoma cells to study PYR actions (0.1-50 μM) under the hypothesis that glial cells may be targeted by and vulnerable to PYRs. To this end, we characterized the effects of bifenthrin (BF), tefluthrin (TF), α-cypermethrin (α-CYP), and deltamethrin (DM) on the integrity of nuclear, mitochondrial, and lysosomal compartments. In general, 24- to 48-h exposures produced concentration-related impairment of cell viability. In single-compound, 24-h exposure experiments, effective concentration (EC)15s 3-(4,5-dimethyl-thiazol-2- yl)-2,5-diphenyl-tetrazolium bromide (MTT assay) were computed as follows (in μM): BF, 16.1; TF, 37.3; α-CYP, 7.8; DM, 5.0. We found concentration-related damage in several C6-cell subcellular compartments (mitochondria, nuclei, and lysosomes) at≥ 10-1 lM levels. Last, we examined a mixture of all PYRs (ie, Σ individual EC15) using MTT assays and subcellular analyses. Our findings indicate that C6 cells are responsive to nM levels of PYRs, suggesting that astroglial susceptibility may contribute to the low-dose neurological effects caused by these insecticides. This research further suggests that C6 cells may provide relevant information as a screening platform for pesticide mixtures targeting nervous system cells by expected and unexpected toxicogenic pathways potentially contributing to clinical neurotoxicity. © The Author 2016. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved.

Registro:

Documento: Artículo
Título:Vulnerability of C6 astrocytoma cells after single-compound and joint exposure to type I and type II pyrethroid insecticides
Autor:Romero, D.M.; Berardino, B.G.; Wolansky, M.J.; Kotler, M.L.
Filiación:Laboratorio de Toxicología de Mezclas Químicas, Universidad de Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
IQUIBICEN-Argentina National Research Council (CONICET), Argentina
Laboratorio de Neuroepigenética;, Argentina
Laboratorio de Disfunción Celular en Enfermedades Neurodegenerativas y Nanomedicina, Argentina
Institut du Fer á Moulin, INSERM UMRS 839, Paris, France
Sorbonne Universités, UniversitéPierre et Marie Curie, Paris, France
Palabras clave:C6 cells; Cell death; Cellular damage; Pyrethroids; 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide; bifenthrin; cypermethrin; deltamethrin; pyrethroid; tefluthrin; unclassified drug; insecticide; pyrethroid; animal cell; Article; astrocytoma cell; cell nucleus; cell viability; controlled study; drug cytotoxicity; glia cell; lysosome; mitochondrion; MTT assay; neurotoxicity; nonhuman; rat; animal; astrocytoma; cell fractionation; dose response; fluorescence microscopy; metabolism; pathology; tumor cell line; Animals; Astrocytoma; Cell Line, Tumor; Dose-Response Relationship, Drug; Insecticides; Microscopy, Fluorescence; Pyrethrins; Rats; Subcellular Fractions
Año:2017
Volumen:155
Número:1
Página de inicio:196
Página de fin:212
DOI: http://dx.doi.org/10.1093/toxsci/kfw188
Título revista:Toxicological Sciences
Título revista abreviado:Toxicol. Sci.
ISSN:10966080
CODEN:TOSCF
CAS:3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide, 298-93-1; bifenthrin, 82657-04-3; cypermethrin, 52315-07-8, 65731-84-2, 66841-24-5, 67375-30-8; deltamethrin, 52918-63-5; Insecticides; Pyrethrins
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_10966080_v155_n1_p196_Romero

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Citas:

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Romero, D.M., Berardino, B.G., Wolansky, M.J. & Kotler, M.L. (2017) . Vulnerability of C6 astrocytoma cells after single-compound and joint exposure to type I and type II pyrethroid insecticides. Toxicological Sciences, 155(1), 196-212.
http://dx.doi.org/10.1093/toxsci/kfw188
---------- CHICAGO ----------
Romero, D.M., Berardino, B.G., Wolansky, M.J., Kotler, M.L. "Vulnerability of C6 astrocytoma cells after single-compound and joint exposure to type I and type II pyrethroid insecticides" . Toxicological Sciences 155, no. 1 (2017) : 196-212.
http://dx.doi.org/10.1093/toxsci/kfw188
---------- MLA ----------
Romero, D.M., Berardino, B.G., Wolansky, M.J., Kotler, M.L. "Vulnerability of C6 astrocytoma cells after single-compound and joint exposure to type I and type II pyrethroid insecticides" . Toxicological Sciences, vol. 155, no. 1, 2017, pp. 196-212.
http://dx.doi.org/10.1093/toxsci/kfw188
---------- VANCOUVER ----------
Romero, D.M., Berardino, B.G., Wolansky, M.J., Kotler, M.L. Vulnerability of C6 astrocytoma cells after single-compound and joint exposure to type I and type II pyrethroid insecticides. Toxicol. Sci. 2017;155(1):196-212.
http://dx.doi.org/10.1093/toxsci/kfw188