Artículo

Godoy Herz, M.A.; Kubaczka, M.G.; Brzyżek, G.; Servi, L.; Krzyszton, M.; Simpson, C.; Brown, J.; Swiezewski, S.; Petrillo, E.; Kornblihtt, A.R. "Light Regulates Plant Alternative Splicing through the Control of Transcriptional Elongation" (2019) Molecular Cell. 73(5):1066-1074.e3
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Abstract:

Light makes carbon fixation possible, allowing plant and animal life on Earth. We have previously shown that light regulates alternative splicing in plants. Light initiates a chloroplast retrograde signaling that regulates nuclear alternative splicing of a subset of Arabidopsis thaliana transcripts. Here, we show that light promotes RNA polymerase II (Pol II) elongation in the affected genes, whereas in darkness, elongation is lower. These changes in transcription are consistent with elongation causing the observed changes in alternative splicing, as revealed by different drug treatments and genetic evidence. The light control of splicing and elongation is abolished in an Arabidopsis mutant defective in the transcription factor IIS (TFIIS). We report that the chloroplast control of nuclear alternative splicing in plants responds to the kinetic coupling mechanism found in mammalian cells, providing unique evidence that coupling is important for a whole organism to respond to environmental cues. Godoy Herz et al. provide biochemical and genetic evidence that plants exposed to light show faster gene transcription than those in the dark. This serves as control for alternative mRNA splicing decisions, which demonstrates that coupling between transcription and splicing is important for a whole organism to respond to environmental cues. © 2018 Elsevier Inc.

Registro:

Documento: Artículo
Título:Light Regulates Plant Alternative Splicing through the Control of Transcriptional Elongation
Autor:Godoy Herz, M.A.; Kubaczka, M.G.; Brzyżek, G.; Servi, L.; Krzyszton, M.; Simpson, C.; Brown, J.; Swiezewski, S.; Petrillo, E.; Kornblihtt, A.R.
Filiación:Universidad de Buenos Aires (UBA), Facultad de Ciencias Exactas y Naturales, Departamento de Fisiología, Biología Molecular y Celular and CONICET-UBA, Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE), (C1428EHA), Buenos Aires, Argentina
Department of Protein Biosynthesis, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
Division of Plant Sciences, University of Dundee at The James Hutton Institute, Invergowrie, Dundee, United Kingdom
Palabras clave:alternative splicing; light control in plants; transcription elongation; histone; messenger RNA; RNA polymerase II; transcription factor II; alternative RNA splicing; Arabidopsis thaliana; Article; carbon fixation; chloroplast; controlled study; darkness; genetic transcription; histone acetylation; intron; light; mammal cell; mutant; nonhuman; plant; regulatory mechanism; transcription elongation
Año:2019
Volumen:73
Número:5
Página de inicio:1066
Página de fin:1074.e3
DOI: http://dx.doi.org/10.1016/j.molcel.2018.12.005
Título revista:Molecular Cell
Título revista abreviado:Mol. Cell
ISSN:10972765
CODEN:MOCEF
CAS:histone, 9062-68-4
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_10972765_v73_n5_p1066_GodoyHerz

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Citas:

---------- APA ----------
Godoy Herz, M.A., Kubaczka, M.G., Brzyżek, G., Servi, L., Krzyszton, M., Simpson, C., Brown, J.,..., Kornblihtt, A.R. (2019) . Light Regulates Plant Alternative Splicing through the Control of Transcriptional Elongation. Molecular Cell, 73(5), 1066-1074.e3.
http://dx.doi.org/10.1016/j.molcel.2018.12.005
---------- CHICAGO ----------
Godoy Herz, M.A., Kubaczka, M.G., Brzyżek, G., Servi, L., Krzyszton, M., Simpson, C., et al. "Light Regulates Plant Alternative Splicing through the Control of Transcriptional Elongation" . Molecular Cell 73, no. 5 (2019) : 1066-1074.e3.
http://dx.doi.org/10.1016/j.molcel.2018.12.005
---------- MLA ----------
Godoy Herz, M.A., Kubaczka, M.G., Brzyżek, G., Servi, L., Krzyszton, M., Simpson, C., et al. "Light Regulates Plant Alternative Splicing through the Control of Transcriptional Elongation" . Molecular Cell, vol. 73, no. 5, 2019, pp. 1066-1074.e3.
http://dx.doi.org/10.1016/j.molcel.2018.12.005
---------- VANCOUVER ----------
Godoy Herz, M.A., Kubaczka, M.G., Brzyżek, G., Servi, L., Krzyszton, M., Simpson, C., et al. Light Regulates Plant Alternative Splicing through the Control of Transcriptional Elongation. Mol. Cell. 2019;73(5):1066-1074.e3.
http://dx.doi.org/10.1016/j.molcel.2018.12.005