Artículo

Steinberg, E.R.; Fortes, V.B.; Rossi, L.F.; Murer, L.; Lovato, M.; Merani, M.S.; Mudry, M.D."Cytogenetic Characterization of Brown Howler Monkeys, Alouatta guariba clamitans (Atelidae, Platyrrhini): Meiotic Confirmation of an X1X1X2X2X3X3/X1X2X3Y1Y2 Sex Chromosome System" (2017) Cytogenetic and Genome Research. 151(3):131-140
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Abstract:

For brown howler monkeys (Alouatta guariba clamitans), diploid chromosome numbers varying from 2n = 45 to 2n = 52, with XX/XY, X1X1X2X2/X1X2Y, and X1X1X2X2X3X3/X1X2X3Y1Y2 sex chromosome systems have been described by mitotic studies but still await confirmation by meiotic analyses. We analyzed 3 male individuals sampled in the wild (in the municipality of Santa Maria, RS, Brazil) as well as 1 male and 1 female individual in captivity at the Saõ Braz breeding center. Peripheral blood samples and testicular biopsies were taken. We found different diploid numbers for both sexes in somatic cells, 2n = 45,X1X2X3Y1Y2 in males and 2n = 46,X1X1X2X2X3X3 in females, with 4 metacentric (9-12), 7 submetacentric (1-6, 8), and 9 acrocentric autosomal chromosome pairs (13-20, 22). X1 and X2 were submetacentric chromosomes, while X3, Y1, and Y2 were acrocentric ones. Spermatocyte microspreads were examined for synaptonemal complexes. Pachytene spermatocyte analysis was done to verify the chromosome number and morphologies observed in mitotic karyotypes. Immunodetection was performed using anti-SMC3 and anti-CREST antibodies. The presence of a sex chromosome pentavalent X1X2X3Y1Y2 in the males was confirmed by C-banding in metaphase I and by immunodetection in prophase I by the clear identification of 5 centromeres. The G-banded karyotype corresponded to that previously described for A. g. clamitans in the south of Brazil (Curitiba, Parana State, and Blumenau, Santa Catarina State) and for the Misiones Province, Argentina. © 2017 S. Karger AG, Basel.

Registro:

Documento: Artículo
Título:Cytogenetic Characterization of Brown Howler Monkeys, Alouatta guariba clamitans (Atelidae, Platyrrhini): Meiotic Confirmation of an X1X1X2X2X3X3/X1X2X3Y1Y2 Sex Chromosome System
Autor:Steinberg, E.R.; Fortes, V.B.; Rossi, L.F.; Murer, L.; Lovato, M.; Merani, M.S.; Mudry, M.D.
Filiación:GIBE, Departamento de Ecologiá, Genética y Evolución, Instituto de Ecologiá, Genética y Evolución de Buenos Aires (IEGEBA-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Guïraldes 2160, Buenos Aires, 1428EGA, Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Instituto de Ecologiá, Genética y Evolución de Buenos Aires (IEGEBA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Laboratorio de Biologiá Cromosómica, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Laboratório de Primatologia, Centro de Educacąõ Superior Norte Do Rio Grande Do sul, Universidade Federal de Santa Maria, Palmeira das Missões, Santa Maria, Brazil
Núcleo de Estudos e Pesquisas em Animais Silvestres (NEPAS), Centro de Ciências Rurais, Universidade Federal de Santa Maria, Santa Maria, Brazil
Palabras clave:Alouatta guariba clamitans; Meiosis; Multiple sex chromosome systems; Sex chromosome pentavalent; adult; Alouatta; Alouatta guariba clamitans; animal experiment; animal tissue; Argentina; Article; C banding; centromere; chromosome deletion; chromosome G band; chromosome number; chromosome pairing; cytogenetics; diploidy; female; geographic distribution; immunodetection; karyotype; male; metaphase; nonhuman; pachytene; priority journal; prophase; somatic cell; spermatocyte; synaptonemal complex; testis biopsy; X chromosome; Y chromosome; Alouatta; animal; chromosome analysis; cytology; genetics; germ cell; meiosis; physiology; sex chromosome; sex determination process; veterinary; Alouatta; Animals; Cytogenetic Analysis; Female; Germ Cells; Male; Meiosis; Sex Chromosomes; Sex Determination Processes; Spermatocytes
Año:2017
Volumen:151
Número:3
Página de inicio:131
Página de fin:140
DOI: http://dx.doi.org/10.1159/000464375
Handle:http://hdl.handle.net/20.500.12110/paper_14248581_v151_n3_p131_Steinberg
Título revista:Cytogenetic and Genome Research
Título revista abreviado:Cytogenet. Genome Res.
ISSN:14248581
CODEN:CGRYA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14248581_v151_n3_p131_Steinberg

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Citas:

---------- APA ----------
Steinberg, E.R., Fortes, V.B., Rossi, L.F., Murer, L., Lovato, M., Merani, M.S. & Mudry, M.D. (2017) . Cytogenetic Characterization of Brown Howler Monkeys, Alouatta guariba clamitans (Atelidae, Platyrrhini): Meiotic Confirmation of an X1X1X2X2X3X3/X1X2X3Y1Y2 Sex Chromosome System. Cytogenetic and Genome Research, 151(3), 131-140.
http://dx.doi.org/10.1159/000464375
---------- CHICAGO ----------
Steinberg, E.R., Fortes, V.B., Rossi, L.F., Murer, L., Lovato, M., Merani, M.S., et al. "Cytogenetic Characterization of Brown Howler Monkeys, Alouatta guariba clamitans (Atelidae, Platyrrhini): Meiotic Confirmation of an X1X1X2X2X3X3/X1X2X3Y1Y2 Sex Chromosome System" . Cytogenetic and Genome Research 151, no. 3 (2017) : 131-140.
http://dx.doi.org/10.1159/000464375
---------- MLA ----------
Steinberg, E.R., Fortes, V.B., Rossi, L.F., Murer, L., Lovato, M., Merani, M.S., et al. "Cytogenetic Characterization of Brown Howler Monkeys, Alouatta guariba clamitans (Atelidae, Platyrrhini): Meiotic Confirmation of an X1X1X2X2X3X3/X1X2X3Y1Y2 Sex Chromosome System" . Cytogenetic and Genome Research, vol. 151, no. 3, 2017, pp. 131-140.
http://dx.doi.org/10.1159/000464375
---------- VANCOUVER ----------
Steinberg, E.R., Fortes, V.B., Rossi, L.F., Murer, L., Lovato, M., Merani, M.S., et al. Cytogenetic Characterization of Brown Howler Monkeys, Alouatta guariba clamitans (Atelidae, Platyrrhini): Meiotic Confirmation of an X1X1X2X2X3X3/X1X2X3Y1Y2 Sex Chromosome System. Cytogenet. Genome Res. 2017;151(3):131-140.
http://dx.doi.org/10.1159/000464375