Di Paolo, M.; Roberti, M.J.; Bordoni, A.V.; Aramendía, P.F.; Wolosiuk, A.; Bossi, M.L. "Nanoporous silica nanoparticles functionalized with a fluorescent turn-on spirorhodamineamide as pH indicators" (2019) Photochemical and Photobiological Sciences. 18(1):155-165
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We prepared water soluble, biocompatible fluorescent turn-on pH nanosensors and characterized their behavior as a function of changes in pH. The response relies on a halochromic reaction of a spirorhodamineamide derived from the bright and highly chemically and photo-stable rhodamine 6G, encapsulated in core/nanoporous shell silica nanoparticles. The fluorescent sensors displayed a fast response in the pH range of intracellular compartments. The encapsulation conferred solubility in aqueous environments and biocompatibility. We assessed the two main properties of the sensor, namely the useful pH range and the kinetics of the response, and compared them to those of the free probe. We found that such properties are strongly dependent on the functionalization and position in the silica matrix relative to the core/shell structure. Finally, we demonstrated the cellular uptake of the nanosensors, and their localization in lysosomes of living cells, by fluorescence confocal microscopy. © The Royal Society of Chemistry and Owner Societies.


Documento: Artículo
Título:Nanoporous silica nanoparticles functionalized with a fluorescent turn-on spirorhodamineamide as pH indicators
Autor:Di Paolo, M.; Roberti, M.J.; Bordoni, A.V.; Aramendía, P.F.; Wolosiuk, A.; Bossi, M.L.
Filiación:INQUIMAE, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Departamento de Química Inorgánica, Analítica y Química Física. Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires, Pabellón 2. Ciudad Universitaria, Ciudad de Buenos Aires, 1428, Argentina
Gerencia Química-Centro Atómico Constituyentes, Comisión Nacional de Energía Atómica, Av. Gral. Paz 1499 B1650KNA San Martín, Buenos Aires, Argentina
Centro de Investigaciones en Bionanociencias “Elizabeth Jares-Erijman” CIBION-CONICET, Godoy Cruz 2390, Ciudad de Buenos Aires, 1425, Argentina
Max-Planck-Institute For Medical Research, Jahnstraße 29, Heidelberg, 69120, Germany
Página de inicio:155
Página de fin:165
Título revista:Photochemical and Photobiological Sciences
Título revista abreviado:Photochem. Photobiol. Sci.


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---------- APA ----------
Di Paolo, M., Roberti, M.J., Bordoni, A.V., Aramendía, P.F., Wolosiuk, A. & Bossi, M.L. (2019) . Nanoporous silica nanoparticles functionalized with a fluorescent turn-on spirorhodamineamide as pH indicators. Photochemical and Photobiological Sciences, 18(1), 155-165.
---------- CHICAGO ----------
Di Paolo, M., Roberti, M.J., Bordoni, A.V., Aramendía, P.F., Wolosiuk, A., Bossi, M.L. "Nanoporous silica nanoparticles functionalized with a fluorescent turn-on spirorhodamineamide as pH indicators" . Photochemical and Photobiological Sciences 18, no. 1 (2019) : 155-165.
---------- MLA ----------
Di Paolo, M., Roberti, M.J., Bordoni, A.V., Aramendía, P.F., Wolosiuk, A., Bossi, M.L. "Nanoporous silica nanoparticles functionalized with a fluorescent turn-on spirorhodamineamide as pH indicators" . Photochemical and Photobiological Sciences, vol. 18, no. 1, 2019, pp. 155-165.
---------- VANCOUVER ----------
Di Paolo, M., Roberti, M.J., Bordoni, A.V., Aramendía, P.F., Wolosiuk, A., Bossi, M.L. Nanoporous silica nanoparticles functionalized with a fluorescent turn-on spirorhodamineamide as pH indicators. Photochem. Photobiol. Sci. 2019;18(1):155-165.