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Abstract:

Fluorescence fluctuation-based methods are non-invasive microscopy tools especially suited for the study of dynamical aspects of biological processes. These methods examine spontaneous intensity fluctuations produced by fluorescent molecules moving through the small, femtoliter-sized observation volume defined in confocal and multiphoton microscopes. The quantitative analysis of the intensity trace provides information on the processes producing the fluctuations that include diffusion, binding interactions, chemical reactions and photophysical phenomena. In this review, we present the basic principles of the most widespread fluctuation-based methods, discuss their implementation in standard confocal microscopes and briefly revise some examples of their applications to address relevant questions in living cells. The ultimate goal of these methods in the Cell Biology field is to observe biomolecules as they move, interact with targets and perform their biological action in the natural context. © 2016 IUBMB Life, 69(1):8–15, 2017. © 2016 International Union of Biochemistry and Molecular Biology

Registro:

Documento: Artículo
Título:Dynamics of intracellular processes in live-cell systems unveiled by fluorescence correlation microscopy
Autor:González Bardeci, N.; Angiolini, J.F.; De Rossi, M.C.; Bruno, L.; Levi, V.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN, UBA-CONICET, Argentina
IFIBA, UBA-CONICET, Argentina
Palabras clave:autocorrelation; cross-correlation; diffusion; fluorescence correlation spectroscopy; fluorescence microscopy; intracellular dynamics; cell nucleus; confocal microscopy; cytology; fluctuations technique; fluorescence correlation microscopy; fluorescence cross correlation spectroscopy; fluorescence microscopy; molecule; nonhuman; practice guideline; quantitative analysis; Review; cells; cytoplasm; fluorescence; molecular imaging; procedures; transport at the cellular level; ultrastructure; Biological Transport; Cells; Cytoplasm; Fluorescence; Microscopy, Fluorescence; Molecular Imaging
Año:2017
Volumen:69
Número:1
Página de inicio:8
Página de fin:15
DOI: http://dx.doi.org/10.1002/iub.1589
Título revista:IUBMB Life
Título revista abreviado:IUBMB Life
ISSN:15216543
CODEN:IULIF
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_15216543_v69_n1_p8_GonzalezBardeci

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Citas:

---------- APA ----------
González Bardeci, N., Angiolini, J.F., De Rossi, M.C., Bruno, L. & Levi, V. (2017) . Dynamics of intracellular processes in live-cell systems unveiled by fluorescence correlation microscopy. IUBMB Life, 69(1), 8-15.
http://dx.doi.org/10.1002/iub.1589
---------- CHICAGO ----------
González Bardeci, N., Angiolini, J.F., De Rossi, M.C., Bruno, L., Levi, V. "Dynamics of intracellular processes in live-cell systems unveiled by fluorescence correlation microscopy" . IUBMB Life 69, no. 1 (2017) : 8-15.
http://dx.doi.org/10.1002/iub.1589
---------- MLA ----------
González Bardeci, N., Angiolini, J.F., De Rossi, M.C., Bruno, L., Levi, V. "Dynamics of intracellular processes in live-cell systems unveiled by fluorescence correlation microscopy" . IUBMB Life, vol. 69, no. 1, 2017, pp. 8-15.
http://dx.doi.org/10.1002/iub.1589
---------- VANCOUVER ----------
González Bardeci, N., Angiolini, J.F., De Rossi, M.C., Bruno, L., Levi, V. Dynamics of intracellular processes in live-cell systems unveiled by fluorescence correlation microscopy. IUBMB Life. 2017;69(1):8-15.
http://dx.doi.org/10.1002/iub.1589