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Electrogenic bacteria are organisms that can transfer electrons to extracellular electron acceptors and have the potential to be used in devices such as bioelectrochemical systems (BES). In this study, Dietzia sp. RNV-4 bacterium has been isolated and identified based on its biochemical, physiological and morphological characteristics, as well as by its 16S rRNA sequence analysis. Furthermore, the current density production and electron transfer mechanisms were investigated using bioelectrochemical methods. The chronoamperometric data showed that the biofilm of Dietzia sp. RNV-4 grew as the current increased with time, reaching a maximum of 176.6 ± 66.1 mA/m2 at the end of the experiment (7 d); this highly suggests that the current was generated by the biofilm. The main electron transfer mechanism, indicated by the cyclic voltammograms, was due to secreted redox mediators. By high performance liquid chromatography, canthaxanthin was identified as the main compound involved in charge transfer between the bacteria and the solid electrodes. Dietzia sp. RNV-4 was used as biological material in a microbial fuel cell (MFC) and the current density production was 299.4 ± 40.2 mA/m2. This is the first time that Dietzia sp. RNV-4 has been electrochemically characterized and identified as a new electrogenic strain. © 2017 Sacco et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Documento: Artículo
Título:Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4
Autor:Sacco, N.J.; Bonetto, M.C.; Cortón, E.
Filiación:Laboratory of Biosensors and Bioanalysis (LABB), Departamento de Química Biológica and IQUIBICENCONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina
Palabras clave:canthaxanthin; RNA 16S; canthaxanthin; RNA 16S; Article; bacterial phenomena and functions; bacterial strain; bacterium isolation; biochemistry; bioelectrochemistry; biofilm; controlled study; cyclic potentiometry; density; Dietzia; Dietzia RNV 4; electrochemistry; electrogenic bacterium; electron transport; high performance liquid chromatography; microbial fuel cell; new species; nonhuman; nucleotide sequence; RNA sequence; Actinobacteria; bioenergy; confocal microscopy; electrochemical analysis; electron; genetics; isolation and purification; metabolism; microbiology; oxidation reduction reaction; phylogeny; procedures; scanning electron microscopy; Actinobacteria; Bioelectric Energy Sources; Biofilms; Canthaxanthin; Electrochemical Techniques; Electron Transport; Electrons; Microscopy, Confocal; Microscopy, Electron, Scanning; Oxidation-Reduction; Phylogeny; RNA, Ribosomal, 16S
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
CAS:canthaxanthin, 514-78-3; Canthaxanthin; RNA, Ribosomal, 16S


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---------- APA ----------
Sacco, N.J., Bonetto, M.C. & Cortón, E. (2017) . Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4. PLoS ONE, 12(2).
---------- CHICAGO ----------
Sacco, N.J., Bonetto, M.C., Cortón, E. "Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4" . PLoS ONE 12, no. 2 (2017).
---------- MLA ----------
Sacco, N.J., Bonetto, M.C., Cortón, E. "Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4" . PLoS ONE, vol. 12, no. 2, 2017.
---------- VANCOUVER ----------
Sacco, N.J., Bonetto, M.C., Cortón, E. Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4. PLoS ONE. 2017;12(2).