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Abstract:

A cytogenetic characterization, including heterochromatin content, and the analysis of the location of rDNA genes, was performed in Largus fasciatus Blanchard, 1843 and L. Rufipennis Laporte, 1832. Mitotic and meiotic analyses revealed the same diploid chromosome number 2n = 12 + X0/XX (male/female). Heterochromatin content, very scarce in both species, revealed C-blocks at both ends of autosomes and X chromosome. The most remarkable cytological feature observed between both species was the different chromosome position of the NORs. This analysis allowed us to use the NORs as a cytological marker because two clusters of rDNA genes are located at one end of one pair of autosomes in L. Fasciatus, whereas a single rDNA cluster is located at one terminal region of the X chromosome in L. Rufipennis. Taking into account our results and previous data obtained in other heteropteran species, the conventional staining, chromosome bandings, and rDNA-FISH provide important chromosome markers for cytotaxonomy, karyotype evolution, and chromosome structure and organization studies. © 2017. Leonardo Luís Artico et al. All reights reserved.

Registro:

Documento: Artículo
Título:A karyotype comparison between two species of bordered plant bugs (hemiptera, heteroptera, largidae) by conventional chromosome staining, c-banding and rDNA-FISH
Autor:Salanitro, L.B.; Massaccesi, A.C.; Urbisaglia, S.; Bressa, M.J.; Chirino, M.G.
Filiación:Laboratorio de Entomología Aplicada y Forense, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Sáenz Peña 352, Bernal, Buenos Aires, B1876BXD, Argentina
Grupo de Citogenética de Insectos,Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, C1428EHA, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas, Godoy Cruz 2290, Ciudad Autónoma de Buenos Aires, C1425FQB, Argentina
Palabras clave:C-banding; Heteroptera; Holokinetic chromosomes; Karyotype comparison; Largus; RDNA-FISH
Año:2017
Volumen:11
Número:2
Página de inicio:239
Página de fin:248
DOI: http://dx.doi.org/10.3897/CompCytogen.v11i2.11683
Título revista:Comparative Cytogenetics
Título revista abreviado:Comp. Cytogenet.
ISSN:19930771
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19930771_v11_n2_p239_Salanitro

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Citas:

---------- APA ----------
Salanitro, L.B., Massaccesi, A.C., Urbisaglia, S., Bressa, M.J. & Chirino, M.G. (2017) . A karyotype comparison between two species of bordered plant bugs (hemiptera, heteroptera, largidae) by conventional chromosome staining, c-banding and rDNA-FISH. Comparative Cytogenetics, 11(2), 239-248.
http://dx.doi.org/10.3897/CompCytogen.v11i2.11683
---------- CHICAGO ----------
Salanitro, L.B., Massaccesi, A.C., Urbisaglia, S., Bressa, M.J., Chirino, M.G. "A karyotype comparison between two species of bordered plant bugs (hemiptera, heteroptera, largidae) by conventional chromosome staining, c-banding and rDNA-FISH" . Comparative Cytogenetics 11, no. 2 (2017) : 239-248.
http://dx.doi.org/10.3897/CompCytogen.v11i2.11683
---------- MLA ----------
Salanitro, L.B., Massaccesi, A.C., Urbisaglia, S., Bressa, M.J., Chirino, M.G. "A karyotype comparison between two species of bordered plant bugs (hemiptera, heteroptera, largidae) by conventional chromosome staining, c-banding and rDNA-FISH" . Comparative Cytogenetics, vol. 11, no. 2, 2017, pp. 239-248.
http://dx.doi.org/10.3897/CompCytogen.v11i2.11683
---------- VANCOUVER ----------
Salanitro, L.B., Massaccesi, A.C., Urbisaglia, S., Bressa, M.J., Chirino, M.G. A karyotype comparison between two species of bordered plant bugs (hemiptera, heteroptera, largidae) by conventional chromosome staining, c-banding and rDNA-FISH. Comp. Cytogenet. 2017;11(2):239-248.
http://dx.doi.org/10.3897/CompCytogen.v11i2.11683