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Abstract:

A cytogenetic characterization, including heterochromatin content, and the analysis of the location of rDNA genes, was performed in Largus fasciatus Blanchard, 1843 and L. Rufipennis Laporte, 1832. Mitotic and meiotic analyses revealed the same diploid chromosome number 2n = 12 + X0/XX (male/female). Heterochromatin content, very scarce in both species, revealed C-blocks at both ends of autosomes and X chromosome. The most remarkable cytological feature observed between both species was the different chromosome position of the NORs. This analysis allowed us to use the NORs as a cytological marker because two clusters of rDNA genes are located at one end of one pair of autosomes in L. Fasciatus, whereas a single rDNA cluster is located at one terminal region of the X chromosome in L. Rufipennis. Taking into account our results and previous data obtained in other heteropteran species, the conventional staining, chromosome bandings, and rDNA-FISH provide important chromosome markers for cytotaxonomy, karyotype evolution, and chromosome structure and organization studies. © 2017. Leonardo Luís Artico et al. All reights reserved.

Registro:

Documento: Artículo
Título:A karyotype comparison between two species of bordered plant bugs (hemiptera, heteroptera, largidae) by conventional chromosome staining, c-banding and rDNA-FISH
Autor:Salanitro, L.B.; Massaccesi, A.C.; Urbisaglia, S.; Bressa, M.J.; Chirino, M.G.
Filiación:Laboratorio de Entomología Aplicada y Forense, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Sáenz Peña 352, Bernal, Buenos Aires, B1876BXD, Argentina
Grupo de Citogenética de Insectos,Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, C1428EHA, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas, Godoy Cruz 2290, Ciudad Autónoma de Buenos Aires, C1425FQB, Argentina
Palabras clave:C-banding; Heteroptera; Holokinetic chromosomes; Karyotype comparison; Largus; RDNA-FISH
Año:2017
Volumen:11
Número:2
Página de inicio:239
Página de fin:248
DOI: http://dx.doi.org/10.3897/CompCytogen.v11i2.11683
Título revista:Comparative Cytogenetics
Título revista abreviado:Comp. Cytogenet.
ISSN:19930771
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_19930771_v11_n2_p239_Salanitro

Referencias:

  • Bardella, V.B., Fernandes, T., Vanzela, A.L.L., The conservation of number and location of 18S sites indicates the relative stability of rDNA in species of Pentatomomorpha (Heterop-tera) (2013) Genome, 56 (7), pp. 425-429
  • Bardella, V.B., Fernandes, J.A.M., Cabral-de-Mello, D.C., Chromosomal evolutionary dynamics of four multigene families in Coreidae and Pentatomidae (Heteroptera) true bugs (2016) Molecular Genetics and Genomics, 291 (5), pp. 1919-1925
  • Bressa, M.J., Papeschi, A.G., Mola, L.M., Larramendy, M.L., Meiotic studies in Largus rufipennis (Castelnau) (Largidae, Heteroptera). II. Reciprocal traslocation heterizygosity (1998) Caryologia, 51, pp. 253-264
  • Bressa, M.J., Larramendy, M.L., Papeschi, A.G., Heterochromatin characterization in five species of Heteroptera (2005) Genetica, 124 (2-3), pp. 307-317
  • Bressa, M.J., Papeschi, A.G., Vítková, M., Kubícková, S., Fuková, I., Pigozzi, M.I., Marec, F., Sex chromosome evolution in cotton stainers of the genus Dysdercus (Heteroptera: Pyrrhocoridae) (2009) Cytogenetic and Genome Research, 125 (4), pp. 292-305
  • Chirino, M.G., Papeschi, A.G., Bressa, M.J., The significance of cytogenetics for the study of karyotype evolution and taxonomy of water bugs (Heteroptera, Belostomatidae) native to Argentina (2013) Comparative Cytogenetics, 7 (2), pp. 9-27
  • Chirino, M.G., Bressa, M.J., Karyotype evolution in progress: A new diploid number in Be-lostoma candidulum (Heteroptera: Belostomatidae) from Argentina leading to new insights into its ecology and evolution (2014) European Journal of Entomology, 111 (2), pp. 165-174
  • Fuková, I., Nguyen, P., Marec, F., Codling moth cytogenetics: Karyotype, chromosomal location of rDNA, and molecular differentiation of sex chromosomes (2005) Genome, 48 (6), pp. 1083-1092
  • Golub, N.V., Golub, V.B., Kuznetsova, V.G., Variability of 18rDNA loci in four lace bug species (Hemiptera, Tingidae) with the same chromosome number (2015) Comparative Cytogenetics, 9 (4), pp. 513-522
  • Golub, N.V., Golub, V.B., Kuznetsova, V.G., Further evidence for the variability of the 18S rDNA loci in the family Tingidae (Hemiptera, Heteroptera) (2016) Comparative Cytogenetics, 10 (4), pp. 517-528
  • Grozeva, S., Nokala, S., Chromosomes and their meiotic behavior in two families of the primitive infraorden Dipsocoromorpha (Heteroptera) (1996) Hereditas, 125 (1), pp. 31-36
  • Grozeva, S., Anokhin, B., Kuznetsova, V.G., Chapter 8. Recent advances in cytogenetics of bed bugs: FISH mapping of the 18S rDNA and TTAGG telomeric loci in Cimex lectu-larius Fabricius, 1803 (Hemiptera, Heteroptera, Cimicidae) (2014) Protocols for chromosome mapping of arthropod genomes, pp. 283-322. , In: Sharachov I (Ed.) CRCPress, Taylor & Francis, Boca Raton, Florida
  • Manna, G.K., Chromosomes in evolution in Heteroptera (1984) Chromosomes in evolution of eukaryotic groups, pp. 189-225. , In: Sharma AK, Sharma A (Eds) CRCPress, Boca Raton, Florida
  • Manna, G.K., Ueshima, N., Dey, S.K., Ded-Mallick, S., Market Sex chromosomal variations between Indian and a Japanese species of Physopelta (Largidae, Heteroptera) (1985) Cytologia, 50, pp. 621-630
  • Melo, M.C., Dellapé, P.M., Catalogue of the Pyrrhocoroidea (Hemiptera: Heteroptera) from Argentina (2013) Revista de la Sociedad Entomológica Argentina, 72 (1-2), pp. 55-74. , http://www.scielo.org.ar/pdf/rsea/v72n1-2/v72n1-2a06.pdf, [In Spanish]
  • Mola, L.M., Papeschi, A.G., Meiotic studies in Largus rufipennis (Castelnau) (Largidae, Heteroptera): Frequency and behaviour of ring bivalents, univalents and B chromosomes (1993) Heredity, 71, pp. 33-40
  • Morielle-Souza, A., Azeredo-Oliveira, M.T.V., Differential characterization of holocentric chromosomes in triatomines (Heteroptera, Triatominae) using different staining techniques and fluorescent in situ hybridization (2007) Genetics and molecular research, 6 (3), pp. 713-720. , https://science.report/pub/16044094
  • Nokkala, S., Nokala, C., Achiasmatic male meiosis in two species of Saldula (Saldidae, Hemiptera) (1983) Hereditas, 99 (1), pp. 131-134
  • Nokkala, S., Nokala, C., The occurrence of the X0 sex chromosome system in Dictyonota tricornis (Schr.) (Tingidae, Hemiptera) and its significance for concepts of sex chromosome system evolution in Heteroptera (1984) Hereditas, 100 (2), pp. 299-301
  • Panzera, F., Ferreiro, M.J., Pita, S., Calleros, L., Pérez, R., Basmadjián, Y., Guevara, Y., Panzera, Y., Evolutionary and dispersal history of Triatoma infestans, main vector of Chagas disease, by chromosomal markers (2014) Infection, Genetics and Evolution, 27, pp. 105-113
  • Papeschi, A.G., C-banding and DNA content in three species of Belostoma (Heteroptera) with large differences in chromosome size and number (1988) Genetica, 76, pp. 43-51
  • Papeschi, A.G., Bressa, M.J., Evolutionary cytogenetics in Heteroptera (2006) Journal of Biological Research, 5, pp. 3-21. , http://www.jbr.gr/papers20061/01-Alba.pdf
  • Pita, S., Panzera, F., Ferrandis, I., Galvao, C., Gomez-Palacio, A., Panzera, Y., Chromosomal divergence and evolutionary inferences in Rhodniini based on the chromosomal location of ribosomal genes (2013) Memórias do Instituto Oswaldo Cruz, 108 (3), pp. 376-382
  • Poggio, M.G., Bressa, M.J., Papeschi, A.G., Male meiosis, heterochromatin characterization and chromosomal location ofrDNA in Microtomus lunifer (Berg, 1900) (Hemiptera: Redu-viidae: Hammacerinae) (2011) Compative Cytogenetics, 5 (1), pp. 1-22
  • Rosas, C., Brailovsky, H., Revision of the genus Largus (Hemiptera: Heteroptera: Largidae) from Mexico (2016) Revista Mexicana de Biodiversidad, 87, pp. 347-375
  • Sahara, K., Marec, F., Traut, W., TTAGG telomeric repeats in chromosomes of some insects and other arthropods (1999) Chromosome Research, 7, pp. 449-460
  • Traut, W., Pachytene mapping in the female silkworm Bombyx mori L. (Lepidoptera) (1976) Chromosoma, 58, pp. 275-284
  • Ueshima, N., (1979) Hemiptera II: Heteroptera, p. 117. , Animal Cytogenetics 3 Insecta 6. Gebrüder Borntraeger, Stuttgart
  • Whiting, M.F., Phylogeny of the holometabolous insect orders: Molecular evidence (2002) Zoologica Scripta, 31 (1), pp. 3-15

Citas:

---------- APA ----------
Salanitro, L.B., Massaccesi, A.C., Urbisaglia, S., Bressa, M.J. & Chirino, M.G. (2017) . A karyotype comparison between two species of bordered plant bugs (hemiptera, heteroptera, largidae) by conventional chromosome staining, c-banding and rDNA-FISH. Comparative Cytogenetics, 11(2), 239-248.
http://dx.doi.org/10.3897/CompCytogen.v11i2.11683
---------- CHICAGO ----------
Salanitro, L.B., Massaccesi, A.C., Urbisaglia, S., Bressa, M.J., Chirino, M.G. "A karyotype comparison between two species of bordered plant bugs (hemiptera, heteroptera, largidae) by conventional chromosome staining, c-banding and rDNA-FISH" . Comparative Cytogenetics 11, no. 2 (2017) : 239-248.
http://dx.doi.org/10.3897/CompCytogen.v11i2.11683
---------- MLA ----------
Salanitro, L.B., Massaccesi, A.C., Urbisaglia, S., Bressa, M.J., Chirino, M.G. "A karyotype comparison between two species of bordered plant bugs (hemiptera, heteroptera, largidae) by conventional chromosome staining, c-banding and rDNA-FISH" . Comparative Cytogenetics, vol. 11, no. 2, 2017, pp. 239-248.
http://dx.doi.org/10.3897/CompCytogen.v11i2.11683
---------- VANCOUVER ----------
Salanitro, L.B., Massaccesi, A.C., Urbisaglia, S., Bressa, M.J., Chirino, M.G. A karyotype comparison between two species of bordered plant bugs (hemiptera, heteroptera, largidae) by conventional chromosome staining, c-banding and rDNA-FISH. Comp. Cytogenet. 2017;11(2):239-248.
http://dx.doi.org/10.3897/CompCytogen.v11i2.11683