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Fasciolosis is a trematode zoonosis of interest in public health and cattle production. We report here the immunostimulatory effect of a 66 mer mucin-like peptide from Fasciola hepatica (Fhmuc), which synergizes with lipopolysaccharide (LPS) to promote dendritic cell (DC) maturation, endowing these cells with Th1-polarizing capacity. Exposure of DCs to Fhmuc in presence of LPS induced enhanced secretion of pro-inflammatory cytokines and expression of co-stimulatory molecules by DCs, promoting their T cell stimulatory capacity and selectively augmenting IFN-3 secretion by allogeneic T cells. Furthermore, exposure of DCs to Fhmuc augmented LPS-induced Toll-like receptor (TLR) 4 expression on the cell surface. Finally, Fhmuc-conditioned DCs induced parasite specific-adaptive immunity with increased levels of IFN-3 secreted by splenocytes from vaccinated animals, and higher parasite-specific IgG antibodies. However, Fhmuc-treated DC conferred modest protection against F. hepatica infection highlighting the potent immuno-regulatory capacity of the parasite. In summary, this work highlights the capacity of a mucin-derived peptide from F. hepatica to enhance LPS-maturation of DCs and induce parasite-specific immune responses with potential implications in vaccination and therapeutic strategies. © The Author(s) 2017.


Documento: Artículo
Título:A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity
Autor:Noya, V.; Brossard, N.; Rodríguez, E.; Dergan-Dylon, L.S.; Carmona, C.; Rabinovich, G.A.; Freire, T.
Filiación:Laboratorio de Inmunomodulación y Desarrollo de Vacunas, Departamento de Inmunobiología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
Laboratorio de Inmunopatología, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina
Unidad de Biología Parasitaria, Instituto de Higiene, Departamento de Biología Celular y Molecular, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:antibody; autacoid; cytokine; gamma interferon; glycoprotein p 15095; helminth antibody; immunoglobulin enhancer binding protein; immunoglobulin G; lipopolysaccharide; mucin 1; peptide; toll like receptor 4; animal; biological model; C57BL mouse; cell polarity; cytology; dendritic cell; drug effect; Fasciola hepatica; female; immunology; lymphocyte activation; metabolism; parasite; pathology; peritoneal cavity; signal transduction; species difference; spleen; Th1 cell; vaccination; Animals; Antibodies; Antibodies, Helminth; CD11c Antigen; Cell Polarity; Cytokines; Dendritic Cells; Fasciola hepatica; Female; Immunoglobulin G; Inflammation Mediators; Interferon-gamma; Lipopolysaccharides; Lymphocyte Activation; Mice, Inbred C57BL; Models, Biological; Mucin-1; NF-kappa B; Parasites; Peptides; Peritoneal Cavity; Signal Transduction; Species Specificity; Spleen; Th1 Cells; Toll-Like Receptor 4; Vaccination
Título revista:Scientific Reports
Título revista abreviado:Sci. Rep.
CAS:gamma interferon, 82115-62-6; immunoglobulin G, 97794-27-9; mucin 1, 212255-06-6; toll like receptor 4, 203811-83-0; Antibodies; Antibodies, Helminth; CD11c Antigen; Cytokines; Immunoglobulin G; Inflammation Mediators; Interferon-gamma; Lipopolysaccharides; Mucin-1; NF-kappa B; Peptides; Toll-Like Receptor 4


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---------- APA ----------
Noya, V., Brossard, N., Rodríguez, E., Dergan-Dylon, L.S., Carmona, C., Rabinovich, G.A. & Freire, T. (2017) . A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity. Scientific Reports, 7.
---------- CHICAGO ----------
Noya, V., Brossard, N., Rodríguez, E., Dergan-Dylon, L.S., Carmona, C., Rabinovich, G.A., et al. "A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity" . Scientific Reports 7 (2017).
---------- MLA ----------
Noya, V., Brossard, N., Rodríguez, E., Dergan-Dylon, L.S., Carmona, C., Rabinovich, G.A., et al. "A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity" . Scientific Reports, vol. 7, 2017.
---------- VANCOUVER ----------
Noya, V., Brossard, N., Rodríguez, E., Dergan-Dylon, L.S., Carmona, C., Rabinovich, G.A., et al. A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity. Sci. Rep. 2017;7.