Artículo

Guillemi, E.C.; Orozco, M.M.; Argibay, H.D.; Farber, M.D. "Evidence of Ehrlichia chaffeensis in Argentina through molecular detection in marsh deer (Blastocerus dichotomus)" (2019) International Journal for Parasitology: Parasites and Wildlife. 8:45-49
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Abstract:

Vector-borne pathogens are responsible for serious emerging diseases and have been widely described in wildlife. Ehrlichia chaffeensis causes the zoonotic “monocytic ehrlichiosis” in humans, is transmitted by the tick Amblyomma americanum and its reservoir host is the white-tailed deer (Odocoileus virginianus) in North America. Little is known about the native reservoir and the tick vectors involved in the transmission cycle in South America. We report here the detection of E. chaffeensis in a study on marsh deer (Blastocerus dichotomus) mortality in Argentina, in different time periods between 2007 and 2016. Four deer, from two distinct populations, were positive for E. chaffeensis through molecular methods. Additionally, the variable-length PCR target (VLPT) region of positive samples was genotyped. Our results provide the first evidence of E. chaffeensis in autochthonous Cervidae from Argentina, contributing to uncover the distribution of this tick-borne infection in South America. © 2018 The Authors

Registro:

Documento: Artículo
Título:Evidence of Ehrlichia chaffeensis in Argentina through molecular detection in marsh deer (Blastocerus dichotomus)
Autor:Guillemi, E.C.; Orozco, M.M.; Argibay, H.D.; Farber, M.D.
Filiación:Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria (INTA), Las Cabañas y Los Reseros s/n (B1712WAA), Castelar, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina
Laboratorio de Ecoepidemiología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Universitaria, Pabellón 2, 2° piso (C1428EHA), Buenos Aires, Argentina
Palabras clave:Blastocerus dichotomus; Ehrlichia chaffeensis; Marsh deer; Ticks; RNA 16S; adult; Amblyomma americanum; amino acid sequence; animal experiment; animal model; Article; autopsy; bacterial transmission; bacterium detection; biosafety; Blastocerus dichotomus; blood sampling; controlled study; deer; DNA extraction; DNA sequence; Ehrlichia chaffeensis; genotype; host pathogen interaction; human monocytic ehrlichiosis; medical examination; molecular diagnosis; mortality; nonhuman; polymerase chain reaction; sequence alignment; skin defect; spectrophotometry; tick infestation; wound fluid
Año:2019
Volumen:8
Página de inicio:45
Página de fin:49
DOI: http://dx.doi.org/10.1016/j.ijppaw.2018.12.004
Título revista:International Journal for Parasitology: Parasites and Wildlife
Título revista abreviado:Int. J. Parasitol. Parasites Wildl.
ISSN:22132244
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_22132244_v8_n_p45_Guillemi

Referencias:

  • Anderson, B.E., Sumner, J.W., Dawson, J.E., Tzianabos, T., Greene, C.R., Olson, J.G., Fishbein, D.B., George, E.H., Detection of the etiologic agent of human ehrlichiosis by polymerase chain reaction (1992) J. Clin. Microbiol., 30 (4), pp. 775-780
  • Bekker, C.P., de Vos, S., Taoufik, A., Sparagano, O.A., Jongejan, F., Simultaneous detection of Anaplasma and Ehrlichia species in ruminants and detection of Ehrlichia ruminantium in Amblyomma variegatum ticks by reverse line blot hybridization (2002) Vet. Microbiol., 89 (2-3), pp. 223-238
  • Cheng, C., Paddock, C.D., Reddy Ganta, R., Molecular heterogeneity of Ehrlichia chaffeensis isolates determined by sequence analysis of the 28-kilodalton outer membrane protein genes and other regions of the genome (2003) Infect. Immun., 71 (1), pp. 187-195
  • Davidson, W.R., Lockhart, J.M., Stallknecht, D.E., Howerth, E.W., Dawson, J.E., Rechav, Y., Persistent Ehrlichia chaffeensis infection in white-tailed deer (2001) J. Wildl. Dis., 37 (3), pp. 538-546
  • Delos Santos, J.R., Oglesbee, M., Rikihisa, Y., Stich, R.W., Pathologic evidence of ehrlichiosis in calves inoculated with Ehrlichia chaffeensis (2008) Ann NYAcad Sci, 1149, pp. 103-106
  • Dumler, J.S., Barbet, A.F., Bekker, C.P.J., Dasch, G.A., Palmer, G.H., Ray, S.C., Rikihisa, Y., Rurangirwa, F.R., Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and ‘HGE agent’ as subjective synonyms of Ehrlichia phagocytophila (2001) Int. J. Syst. Evol. Microbiol., 51, pp. 2145-2165
  • Ewing, S.A., Dawson, J.E., Kocan, A.A., Barker, R.W., Warner, C.K., Panciera, R.J., Fox, J.C., Blouin, E.F., Experimental transmission of Ehrlichia chaffeensis (Rickettsiales: Ehrlichieae) among white-tailed deer by Amblyomma americanum (Acari: Ixodidae) (1995) J. Med. Entomol., 32 (3), pp. 368-374
  • Guglielmone, A.A., Viñabal, A.E., Claves morfológicas dicotómicas e información ecológica para la identificación de garrapatas del género Amblyomma Koch, 1844 de la Argentina (1994) Revista de Investigaciones Agropecuarias, 25, pp. 39-67
  • Halos, L., Jamal, T., Vial, L., Maillard, R., Suau, A., Le Menach, A., Boulouis, H.J., Vayssier-Taussat, M., Determination of an efficient and reliable method for DNA extraction from ticks (2004) Vet. Res., 35 (6), pp. 709-713
  • Iriondo, M., The littoral complex at the Paraná mouth (2004) Quat. Int., 114, pp. 143-154
  • Machado, R.Z., Duarte, J.M., Dagnone, A.S., Szabo, M.P., Detection of Ehrlichia chaffeensis in Brazilian marsh deer (Blatocercus dichotomus) (2006) Vet. Parasitol., 139 (1-3), pp. 262-266
  • Malvárez, A.I., El Delta del Río Paraná como mosaico de humedales. IV (1999) Tópicos sobre humedales subtropicales y templados de Sudamérica. Oficina Regional de Ciencia y Técnica para América Latina y el Caribe, pp. 35-54. , A.I. Malvárez MAB/UNESCO Montevideo, Uruguay
  • Nava, S., Beati, L., Labruna, M.B., Caceres, A.G., Mangold, A.J., Guglielmone, A.A., Reassessment of the taxonomic status of Amblyomma cajennense with the description of three new species, Amblyomma tonelliae n. sp., Amblyomma interandinum n. sp. and Amblyomma patinoi sp., and reinstatement of Amblyomma mixtum, and Amblyomma sculptum (Ixodida: Ixodidae) (2014) Ticks Tick Borne Dis, 5, pp. 252-276
  • Orozco, M.M., Marull, C., Jiménez Pérez, I., Gürtler, R., Winter mortality of marsh deer (Blastocerus dichotomus, Illiger 1815) in wetlands of northeastern Argentina (2013) Mastozool. Neotrop., 20 (1), pp. 163-170
  • Paddock, C.D., Childs, J.E., Ehrlichia chaffeensis: a prototypical emerging pathogen (2003) Clin. Microbiol. Rev., 16 (1), pp. 37-64
  • Paddock, C.D., Yabsley, M.J., Ecological havoc, the rise of white- tailed deer, and the emergence of Amblyomma americanum associated zoonoses in the United States (2007) Curr. Top. Microbiol. Immunol., 315, pp. 289-324
  • Pritt, B.S., Allerdice, M.E.J., Sloan, L.M., Paddock, C.D., Munderloh, U.G., Rikihisa, Y., Tajima, T., Karpathy, S.E., Proposal to reclassify Ehrlichia muris as Ehrlichia muris subsp. muris subsp. nov. and description of Ehrlichia muris subsp. eauclairensis subsp. nov., a newly recognized tick-borne pathogen of humans (2017) Int. J. Syst. Evol. Microbiol., 67 (7), pp. 2121-2126
  • Randolph, S.E., To what extent has climate change contributed to the recent epidemiology of tick-borne diseases? (2010) Vet. Parasitol., 167 (2-4), pp. 92-94
  • Ripoll, C.M., Remondegui, C.E., Ordonez, G., Arazamende, R., Fusaro, H., Hyman, M.J., Paddock, C.D., Santos-Buch, C.A., Evidence of rickettsial spotted fever and ehrlichial infections in a subtropical territory of Jujuy, Argentina (1999) Am. J. Trop. Med. Hyg., 61 (2), pp. 350-354
  • Sacchi, A.B., Duarte, J.M., André, M.R., Machado, R.Z., Prevalence and molecular characterization of Anaplasmataceae agents in free-ranging Brazilian marsh deer (Blastocerus dichotomus) (2012) Comp. Immunol. Microbiol. Infect. Dis., 35 (4), pp. 325-334
  • Sumner, J.W., Childs, J.E., Paddock, C.D., Molecular cloning and characterization of the Ehrlichia chaffeensis Variable-Length PCR Target: an antigen-expressing gene that exhibits interstrain variation (1999) J. Clin. Microbiol., 37 (5), pp. 1447-1453
  • Tate, C.M., Howerth, E.W., Mead, D.G., Dugan, V.G., Luttrell, M.P., Sahora, A.I., Munderloh, U.G., Yabsley, M.J., Anaplasma odocoilei sp. nov. (family Anaplasmataceae) from white-tailed deer (Odocoileus virginianus) (2013) Ticks Tick Borne Dis, 4 (1-2), pp. 110-119
  • Tomassone, L., Nuñez, P., Gürtler, R.E., Ceballos, L.A., Orozco, M.M., Kitron, U.D., Farber, M., Molecular detection of Ehrlichia chaffeensis in Amblyomma parvum ticks collected in northern Argentina (2008) Emerg. Infect. Dis., 14 (12), pp. 1953-1955
  • Yabsley, M.J., Natural History of Ehrlichia chaffeensis: vertebrate hosts and tick vectors from the United States and evidence for endemic transmission in other countries (2010) Vet. Parasitol., 167 (2-4), pp. 136-148
  • Yang, J., Liu, Z., Niu, Q., Liu, J., Han, R., Guan, G., Hassan, M.A., Yin, H., A novel zoonotic Anaplasma species is prevalent in small ruminants: potential public health implications (2017) Parasites Vectors, 10 (1), p. 264

Citas:

---------- APA ----------
Guillemi, E.C., Orozco, M.M., Argibay, H.D. & Farber, M.D. (2019) . Evidence of Ehrlichia chaffeensis in Argentina through molecular detection in marsh deer (Blastocerus dichotomus). International Journal for Parasitology: Parasites and Wildlife, 8, 45-49.
http://dx.doi.org/10.1016/j.ijppaw.2018.12.004
---------- CHICAGO ----------
Guillemi, E.C., Orozco, M.M., Argibay, H.D., Farber, M.D. "Evidence of Ehrlichia chaffeensis in Argentina through molecular detection in marsh deer (Blastocerus dichotomus)" . International Journal for Parasitology: Parasites and Wildlife 8 (2019) : 45-49.
http://dx.doi.org/10.1016/j.ijppaw.2018.12.004
---------- MLA ----------
Guillemi, E.C., Orozco, M.M., Argibay, H.D., Farber, M.D. "Evidence of Ehrlichia chaffeensis in Argentina through molecular detection in marsh deer (Blastocerus dichotomus)" . International Journal for Parasitology: Parasites and Wildlife, vol. 8, 2019, pp. 45-49.
http://dx.doi.org/10.1016/j.ijppaw.2018.12.004
---------- VANCOUVER ----------
Guillemi, E.C., Orozco, M.M., Argibay, H.D., Farber, M.D. Evidence of Ehrlichia chaffeensis in Argentina through molecular detection in marsh deer (Blastocerus dichotomus). Int. J. Parasitol. Parasites Wildl. 2019;8:45-49.
http://dx.doi.org/10.1016/j.ijppaw.2018.12.004