Registro:
| Documento: | Tesis Doctoral |
| Disciplina: | quimica |
| Título: | Clonado y caracterización de quinasas relacionadas con la CDC2 en el parásito Trypanosoma cruzi |
| Título alternativo: | Cloning and characterization of CDC2 related protein kinases from the parasite Trypanosoma cruzi |
| Autor: | Gómez, Eliana Beatriz |
| Editor: | Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
| Filiación: | Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, "Dr. Héctor N. Torres" (INGEBI)
|
| Publicación en la Web: | 2017-03-01 |
| Fecha de defensa: | 1999 |
| Fecha en portada: | 1999 |
| Grado Obtenido: | Doctorado |
| Título Obtenido: | Doctor en Ciencias Químicas |
| Director: | Téllez-Iñón, María Teresa |
| Idioma: | Español |
| Tema: | biología/biología molecular y celular
biología/parasitología
|
| Formato: | PDF |
| Handle: |
http://hdl.handle.net/20.500.12110/tesis_n3128_Gomez |
| PDF: | https://bibliotecadigital.exactas.uba.ar/download/tesis/tesis_n3128_Gomez.pdf |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/tesis/document/tesis_n3128_Gomez |
| Ubicación: | Dep.BIO 003128 |
| Derechos de Acceso: | Esta obra puede ser leída, grabada y utilizada con fines de estudio, investigación y docencia. Es necesario el reconocimiento de autoría mediante la cita correspondiente. Gómez, Eliana Beatriz. (1999). Clonado y caracterización de quinasas relacionadas con la CDC2 en el parásito Trypanosoma cruzi. (Tesis Doctoral. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales.). Recuperado de http://hdl.handle.net/20.500.12110/tesis_n3128_Gomez |
Abstract:
Two CDC2-related protein kinase genes (crk), tzcrk3 and tzcrk1, were cloned from the parasite Trypanosoma cruzi. Recombinant proteins were used to raise specific antisera. Antibodies anti-TzCRK1recognized a protein of 33 kDa in the three forms of the parasite. This antiserumshowed that TzCRK1 is localized in the cytosol, kinetoplast and nucleus, and is constitutiverexpressed though the cell cycle. Purified anti-TzCRK1 IgGs, immunoprecipitated a kinaseactivity which phosphorylated histone H1 and Rb. The recombinant GST-TzCRK1 had high invitro kinase activity in the absense of regulatory proteins. CDC2 and CDKZ specific inhibitorscould not abolish the TzCRK1 kinase activity. Transfection of COS-7 cells with tzcrk1demonstrated for the first time that a CRK protein can bind mammalian cyclins; TzCRK1 co-immunoprecipitatedwith cyclins E, D3 and A suggesting a role for this kinase in cell cyclecontrol. The TzCRK3 antisera recognized a 40 kDa protein in membrane and nuclear fractionsand a 54 kDa protein in the citosolic fraction. The p13SUC1 protein coprecipitated a kinaseactivity from the citosol of the epimatigote stage. Yeast complementation assays showed thatthese T. cruzi CRKs, could not rescue the yeast CDC28 function. The two hybrid system was used to identify and clone proteins that associate to TzCRK1. Threeclones were identified, which code for proteins with identity to cyclins from the PREG and PHo80 family. In other organisms, these cyclin like proteins are involved in the control ofphosphate metabolism. In S. cerevisiae, PH080 binds to the CDK protein PHO85, whichcontrols the transcription of the acid phosphatase gene and participates in the regulation of thecell cycle. These results suggest that TzCRK1 could be involved in cell cycle progression and/orin other processes such as phosphate metabolism.
Citación:
---------- APA ----------
Gómez, Eliana Beatriz. (1999). Clonado y caracterización de quinasas relacionadas con la CDC2 en el parásito Trypanosoma cruzi. (Tesis Doctoral. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales.). Recuperado de https://hdl.handle.net/20.500.12110/tesis_n3128_Gomez
---------- CHICAGO ----------
Gómez, Eliana Beatriz. "Clonado y caracterización de quinasas relacionadas con la CDC2 en el parásito Trypanosoma cruzi". Tesis Doctoral, Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales, 1999.https://hdl.handle.net/20.500.12110/tesis_n3128_Gomez
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https://bibliotecadigital.exactas.uba.ar/download/tesis/tesis_n3128_Gomez.pdf
