Artículo
Roberti, M.J.; Morgan, M.; Menéndez, G.; Pietrasanta, L.I.; Jovin, T.M.; Jares-Erijman, E.A. "Quantum dots as ultrasensitive nanoactuators and sensors of amyloid aggregation in live cells" (2009) Journal of the American Chemical Society. 131(23):8102-8107
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Abstract:
Quantum dots multifunctionalized with the amyloid protein α-synuclein act at nanomolar concentrations as very potent inducers of the aggregation of micromolar-millimolar bulk concentrations of the protein in vitro and in cells. Fibrillation in live cells, a process diagnostic of Parkinson's disease, is accelerated up to 15-fold with only ∼100 nanoparticles. The combination with a tetracysteine-tagged form of α-synuclein specific for fluorogenic biarsenicals constitutes a very sensitive system for studying pathological amyloid formation in cells. © 2009 American Chemical Society.
Registro:
| Documento: | Artículo |
| Título: | Quantum dots as ultrasensitive nanoactuators and sensors of amyloid aggregation in live cells |
| Autor: | Roberti, M.J.; Morgan, M.; Menéndez, G.; Pietrasanta, L.I.; Jovin, T.M.; Jares-Erijman, E.A. |
| Filiación: | Departamento de Química Orgánica, Facultad de Ciencias Exactas Y Naturales (FCEyN), CONICET, Buenos Aires, Argentina Laboratory of Cellular Dynamics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany DFG Forschungszentrum CMPB Molecular Physiology of the Brain, Göttingen, Germany Laboratorio Max Planck de Dinámica Celular, FCEyN, UBA, Buenos Aires, Argentina Centro de Microscopías Avanzadas, CONICET, UBA, Buenos Aires, Argentina |
| Palabras clave: | Amyloid formation; Amyloid proteins; Bulk concentration; Fluorogenic; In-cell; In-vitro; Live cell; Nanoactuators; Nanomolar concentration; Parkinson's disease; Process diagnostics; Quantum Dot; Sensitive systems; Synuclein; Ultrasensitive; Glycoproteins; Semiconductor quantum dots; Cell membranes; alpha synuclein; amyloid; amyloid protein; nanoparticle; quantum dot; fluorescent dye; article; atomic force microscopy; concentration (parameters); female; fluorescence microscopy; human; human cell; in vitro study; molecular mechanics; nanosensor; Parkinson disease; protein aggregation; quantum mechanics; chemistry; genetic procedures; HeLa cell; metabolism; alpha-Synuclein; Amyloid; Biosensing Techniques; Fluorescent Dyes; Hela Cells; Humans; Microscopy, Atomic Force; Microscopy, Fluorescence; Quantum Dots |
| Año: | 2009 |
| Volumen: | 131 |
| Número: | 23 |
| Página de inicio: | 8102 |
| Página de fin: | 8107 |
| DOI: | http://dx.doi.org/10.1021/ja900225w |
| Título revista: | Journal of the American Chemical Society |
| Título revista abreviado: | J. Am. Chem. Soc. |
| ISSN: | 00027863 |
| CODEN: | JACSA |
| CAS: | alpha synuclein, 154040-18-3; amyloid, 11061-24-8; Amyloid; Fluorescent Dyes; alpha-Synuclein |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00027863_v131_n23_p8102_Roberti |
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Citas:
---------- APA ----------
Roberti, M.J., Morgan, M., Menéndez, G., Pietrasanta, L.I., Jovin, T.M. & Jares-Erijman, E.A. (2009) . Quantum dots as ultrasensitive nanoactuators and sensors of amyloid aggregation in live cells. Journal of the American Chemical Society, 131(23), 8102-8107.http://dx.doi.org/10.1021/ja900225w
---------- CHICAGO ----------
Roberti, M.J., Morgan, M., Menéndez, G., Pietrasanta, L.I., Jovin, T.M., Jares-Erijman, E.A. "Quantum dots as ultrasensitive nanoactuators and sensors of amyloid aggregation in live cells" . Journal of the American Chemical Society 131, no. 23 (2009) : 8102-8107.http://dx.doi.org/10.1021/ja900225w
---------- MLA ----------
Roberti, M.J., Morgan, M., Menéndez, G., Pietrasanta, L.I., Jovin, T.M., Jares-Erijman, E.A. "Quantum dots as ultrasensitive nanoactuators and sensors of amyloid aggregation in live cells" . Journal of the American Chemical Society, vol. 131, no. 23, 2009, pp. 8102-8107.http://dx.doi.org/10.1021/ja900225w
---------- VANCOUVER ----------
Roberti, M.J., Morgan, M., Menéndez, G., Pietrasanta, L.I., Jovin, T.M., Jares-Erijman, E.A. Quantum dots as ultrasensitive nanoactuators and sensors of amyloid aggregation in live cells. J. Am. Chem. Soc. 2009;131(23):8102-8107.http://dx.doi.org/10.1021/ja900225w
