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Abstract:

Native chloroplast fructose-1,6-bisphosphatase (d-fructose 1,6-bisphosphate-l-phosphohydrolase, EC 3.1.3.11) wasinactivated with either Woodward's reagent K or diethylpyrocarbonate. Since pseudo-first-order rate constants (κapp) were a hyperbolic function of inhibitor concentration, it appeared that native chloroplast fructose-1,6-bisphosphatase and either Woodward's reagent K or diethylpyrocarbonate interacted reversibly prior to formation of irreversible (inactive) complex. Protection against inactivation was afforded by preincubating the enzyme with dithiothreitol, fructose 1,6-bisphosphate, Ca2+ and either chloroplast thioredoxin-f or a chaotropic anion (trichloroacetate). Following similar incubation with an organic solvent (2-propanol), the catalytic activity remained unaltered in the presence of Woodward's reagent K but was inactivated by diethylpyrocarbonate. The enhancement of the specific activity of chloroplast fructose-1,6-bisphosphatase caused by dithiothreitol, fructose-1,6-bisphosphate, Ca2+ and either chloroplast thioredoxin-f, or chaotropic anions or cosolvents was similar. Therefore, differential reactivity to selected reagents indicated the existence of several conformations of chloroplast fructose-1,6-bisphosphatase, i.e., native and different active forms. © 1989 Elsevier Science Publishers B.V. (Biomedical Division).

Registro:

Documento: Artículo
Título:Differential reactivity of chloroplast fructose-l,6-bisphosphatase to Woodward's reagent K and diethylpyrocarbonate
Autor:Prat-Gay, G.; Wolosiuk, RicardoA.
Filiación:Institulo de Iiwestigaciones Bioqu'imicas, Facullad de Ciencias Exailasy Naturales (U.B.A.). Buenos Aires, Argentina
Palabras clave:Chaotropic anion; Diethylpyrocarbonate: Thioredoxin; Fructose-l,6-bisphosphalase: Woodward's reagent K; Organic solvent
Año:1989
Volumen:973
Número:3
Página de inicio:457
Página de fin:464
DOI: http://dx.doi.org/10.1016/S0005-2728(89)80388-3
Título revista:BBA - Bioenergetics
Título revista abreviado:Biochim. Biophys. Acta Bioenerg.
ISSN:00052728
CODEN:BBBEB
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052728_v973_n3_p457_PratGay

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Citas:

---------- APA ----------
Prat-Gay, G. & Wolosiuk, RicardoA. (1989) . Differential reactivity of chloroplast fructose-l,6-bisphosphatase to Woodward's reagent K and diethylpyrocarbonate. BBA - Bioenergetics, 973(3), 457-464.
http://dx.doi.org/10.1016/S0005-2728(89)80388-3
---------- CHICAGO ----------
Prat-Gay, G., Wolosiuk, RicardoA. "Differential reactivity of chloroplast fructose-l,6-bisphosphatase to Woodward's reagent K and diethylpyrocarbonate" . BBA - Bioenergetics 973, no. 3 (1989) : 457-464.
http://dx.doi.org/10.1016/S0005-2728(89)80388-3
---------- MLA ----------
Prat-Gay, G., Wolosiuk, RicardoA. "Differential reactivity of chloroplast fructose-l,6-bisphosphatase to Woodward's reagent K and diethylpyrocarbonate" . BBA - Bioenergetics, vol. 973, no. 3, 1989, pp. 457-464.
http://dx.doi.org/10.1016/S0005-2728(89)80388-3
---------- VANCOUVER ----------
Prat-Gay, G., Wolosiuk, RicardoA. Differential reactivity of chloroplast fructose-l,6-bisphosphatase to Woodward's reagent K and diethylpyrocarbonate. Biochim. Biophys. Acta Bioenerg. 1989;973(3):457-464.
http://dx.doi.org/10.1016/S0005-2728(89)80388-3