Artículo
Abstract:
Incubation of UDP-[14C]Glc with the inner membranes of Agrobacterium tumefaciens leads to the formation of cyclic β1-2 glucan and trichloroacetic acid-insoluble compounds. The proteolysis products of the latter show a positive charge in acid and a negative charge in alkaline buffers. The cyclic β1-2 glucan and the trichloroacetic acid insoluble compounds yield the same products on partial acid hydrolysis. Addition of excess non-radioactive UDP-Glc to the reaction mixture nearly stops the formation of radioactive β1-2 glucan and leads to a rapid fall of radioactivity in the trichloroacetic acid precipitate. Alkaline treatment of the insoluble compounds under conditions of β-elimination leads to the partial release of free saccharides (about 30%). It is concluded that β1-2 glucan chains are built up joined to a protein and then released as free cyclic β1-2 glucan. © 1985.
Registro:
| Documento: | Artículo |
| Título: | An intermediate in cyclic β1-2 glucan biosynthesis |
| Autor: | Zorreguieta, A.; Ugalde, R.A.; Leloir, L.F. |
| Filiación: | Instituto de Investigaciones Bioquimicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales Antonio Machado 151, 1405 Buenos Aires, Argentina CONICET, Antonio Machado 151, 1405 Buenos Aires, Argentina |
| Palabras clave: | radioisotope; agrobacterium tumefaciens; beta 1,2 glucan; nonhuman; priority journal; uridine diphosphate glucose c 14; Agrobacterium; Chromatography, Paper; Electrophoresis, Polyacrylamide Gel; Glucans; Hydrogen-Ion Concentration; Models, Chemical |
| Año: | 1985 |
| Volumen: | 126 |
| Número: | 1 |
| Página de inicio: | 352 |
| Página de fin: | 357 |
| DOI: | http://dx.doi.org/10.1016/0006-291X(85)90613-8 |
| Título revista: | Biochemical and Biophysical Research Communications |
| Título revista abreviado: | Biochem. Biophys. Res. Commun. |
| ISSN: | 0006291X |
| CODEN: | BBRCA |
| CAS: | beta-1,2-glucan, 9051-99-4; Glucans |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v126_n1_p352_Zorreguieta |
Referencias:
- Koizumi, Okada, Horiyama, Utamura, Hisamatsu, Amemura, (1983) J. Chromatogr, 265, pp. 89-96
- Dell, York, McNeil, Darvill, Albersheim, (1983) Carbohydr. Res, 117, pp. 185-200
- Amemura, Synthesis of (1.RAR.2)-.BETA.-D-glucan by cell-free extracts of Agrobacterium radiobacter IFO 12665b1 and rhizobium phaseoli AHU 1133. (1984) Agricultural and Biological Chemistry, 48, pp. 1809-1817
- Zorreguieta, A., Tolmasky, M.E. and Staneloni, R.J. Submitted for publication; Dedonder, Hassid, (1964) Biochim. Biophys. Acta, 90, pp. 239-248
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Citas:
---------- APA ----------
Zorreguieta, A., Ugalde, R.A. & Leloir, L.F. (1985) . An intermediate in cyclic β1-2 glucan biosynthesis. Biochemical and Biophysical Research Communications, 126(1), 352-357.http://dx.doi.org/10.1016/0006-291X(85)90613-8
---------- CHICAGO ----------
Zorreguieta, A., Ugalde, R.A., Leloir, L.F. "An intermediate in cyclic β1-2 glucan biosynthesis" . Biochemical and Biophysical Research Communications 126, no. 1 (1985) : 352-357.http://dx.doi.org/10.1016/0006-291X(85)90613-8
---------- MLA ----------
Zorreguieta, A., Ugalde, R.A., Leloir, L.F. "An intermediate in cyclic β1-2 glucan biosynthesis" . Biochemical and Biophysical Research Communications, vol. 126, no. 1, 1985, pp. 352-357.http://dx.doi.org/10.1016/0006-291X(85)90613-8
---------- VANCOUVER ----------
Zorreguieta, A., Ugalde, R.A., Leloir, L.F. An intermediate in cyclic β1-2 glucan biosynthesis. Biochem. Biophys. Res. Commun. 1985;126(1):352-357.http://dx.doi.org/10.1016/0006-291X(85)90613-8
