Abstract:
A macromolecular (1→4)-α-d-[14C]glucan-protein complex was synthesized with a rat liver preparation and uridine diphosphate d-[14C]glucose. The size of the complex is contributed by both the protein and the (1→4)-α-d-glucosyl-oligomer components. Iodoacetamide treatment did not change the migration properties on Bio-Gel A-50m. Therefore, disulfide bonds linking glucan-protein subunits seem not to be involved. The [14C]glucan-protein, precipitated by diluted trichloroacetic acid, was digested by α-amylase, phosphorylase a, and proteases. The extent of proteolysis was greater for a complex having fewer d-glucose units incorporated. After proteolytic digestion of that complex, the labeled fragments behaved on electrophoresis, and ion-exchange and gel chromatography as [14C]glucosylated peptides. These findings support previous conclusions that the primer for liver glycogen synthesis is a protein on which glycogen is built up by covalent attachment. © 1986.
Registro:
Documento: |
Artículo
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Título: | A (1→4)-α-d-glucan-protein involved in liver glycogen biosynthesis |
Autor: | Krisman, C.R.; Geremia, R.A.; Whelan, W.J. |
Filiación: | Instituto de Investigaciones Bioquímicas Fundacion Compamar, Facultad de Ciencias Exactas y Naturales, CONICET, Antonio Machado 151, 1405 Buenos Aires, Argentina Department of Biochemistry, University of Miami School of Medicine, Miami, FL 33101, United States
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Palabras clave: | carbon; glucan; glycoprotein; uridine diphosphate glucose; animal; article; biosynthesis; enzymology; gel chromatography; glycogen liver level; ion exchange chromatography; liver; metabolism; paper electrophoresis; rat; Animal; Carbon Radioisotopes; Chromatography, Gel; Chromatography, Ion Exchange; Electrophoresis, Paper; Glucans; Glycoproteins; Liver; Liver Glycogen; Rats; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.; Uridine Diphosphate Glucose |
Año: | 1986
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Volumen: | 149
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Número: | 1
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Página de inicio: | 35
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Página de fin: | 45
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DOI: |
http://dx.doi.org/10.1016/S0008-6215(00)90367-7 |
Título revista: | Carbohydrate Research
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Título revista abreviado: | Carbohydr. Res.
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ISSN: | 00086215
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CODEN: | CRBRA
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CAS: | carbon, 7440-44-0; glucan, 9012-72-0, 9037-91-6; uridine diphosphate glucose, 133-89-1; Carbon Radioisotopes; Glucans; Glycoproteins; Liver Glycogen; Uridine Diphosphate Glucose, 133-89-1
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v149_n1_p35_Krisman |
Referencias:
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Citas:
---------- APA ----------
Krisman, C.R., Geremia, R.A. & Whelan, W.J.
(1986)
. A (1→4)-α-d-glucan-protein involved in liver glycogen biosynthesis. Carbohydrate Research, 149(1), 35-45.
http://dx.doi.org/10.1016/S0008-6215(00)90367-7---------- CHICAGO ----------
Krisman, C.R., Geremia, R.A., Whelan, W.J.
"A (1→4)-α-d-glucan-protein involved in liver glycogen biosynthesis"
. Carbohydrate Research 149, no. 1
(1986) : 35-45.
http://dx.doi.org/10.1016/S0008-6215(00)90367-7---------- MLA ----------
Krisman, C.R., Geremia, R.A., Whelan, W.J.
"A (1→4)-α-d-glucan-protein involved in liver glycogen biosynthesis"
. Carbohydrate Research, vol. 149, no. 1, 1986, pp. 35-45.
http://dx.doi.org/10.1016/S0008-6215(00)90367-7---------- VANCOUVER ----------
Krisman, C.R., Geremia, R.A., Whelan, W.J. A (1→4)-α-d-glucan-protein involved in liver glycogen biosynthesis. Carbohydr. Res. 1986;149(1):35-45.
http://dx.doi.org/10.1016/S0008-6215(00)90367-7