Artículo

Zitare, U.A.; Szuster, J.; Santalla, M.C.; Llases, M.E.; Morgada, M.N.; Vila, A.J.; Murgida, D.H. "Fine Tuning of Functional Features of the Cu A Site by Loop-Directed Mutagenesis" (2019) Inorganic Chemistry. 58(3):2149-2157
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Abstract:

Here we report the spectroscopic and electrochemical characterization of three novel chimeric Cu A proteins in which either one or the three loops surrounding the metal ions in the Thermus thermophilus protein have been replaced by homologous human and plant sequences while preserving the set of coordinating amino acids. These conservative modifications mimic basic differences between Cu A sites from different organisms and allow for fine tuning the energy gap between alternative electronic ground states of Cu A. . This results in a systematic modulation of thermodynamic and kinetic electron transfer (ET) parameters and in the selection of one of two possible redox-active molecular orbitals, which differ in the ET reorganization energy by a factor of 2. Moreover, the ET mechanism is found to be frictionally controlled, and the modifications introduced into the different chimeras do not affect the frictional activation parameter. © 2019 American Chemical Society.

Registro:

Documento: Artículo
Título:Fine Tuning of Functional Features of the Cu A Site by Loop-Directed Mutagenesis
Autor:Zitare, U.A.; Szuster, J.; Santalla, M.C.; Llases, M.E.; Morgada, M.N.; Vila, A.J.; Murgida, D.H.
Filiación:Departamento de Química Inorgánica, Analítica y Química Física, Facultad de Ciencias Exactas y Naturales, Instituto de Química Física de Los Materiales, Medio Ambiente y Energía, Universidad de Buenos Aires, CONICET, Buenos Aires, 1428, Argentina
Departamento de Química Biológica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Instituto de Biología Molecular y Celular de Rosario (IBR), Universidad Nacional de Rosario, CONICET, Rosario, 2000, Argentina
Univ Rennes, CNRS, ISCR (Institut des Sciences Chimiques de Rennes), UMR 6226, Rennes, F-35000, France
Palabras clave:copper; cytochrome c oxidase; chemistry; electrochemical analysis; electron transport; kinetics; metabolism; molecular model; thermodynamics; Thermus thermophilus; X ray crystallography; Copper; Crystallography, X-Ray; Electrochemical Techniques; Electron Transport; Electron Transport Complex IV; Kinetics; Models, Molecular; Thermodynamics; Thermus thermophilus
Año:2019
Volumen:58
Número:3
Página de inicio:2149
Página de fin:2157
DOI: http://dx.doi.org/10.1021/acs.inorgchem.8b03244
Título revista:Inorganic Chemistry
Título revista abreviado:Inorg. Chem.
ISSN:00201669
CODEN:INOCA
CAS:copper, 15158-11-9, 7440-50-8; cytochrome c oxidase, 72841-18-0, 9001-16-5; Copper; Electron Transport Complex IV
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_00201669_v58_n3_p2149_Zitare

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Citas:

---------- APA ----------
Zitare, U.A., Szuster, J., Santalla, M.C., Llases, M.E., Morgada, M.N., Vila, A.J. & Murgida, D.H. (2019) . Fine Tuning of Functional Features of the Cu A Site by Loop-Directed Mutagenesis. Inorganic Chemistry, 58(3), 2149-2157.
http://dx.doi.org/10.1021/acs.inorgchem.8b03244
---------- CHICAGO ----------
Zitare, U.A., Szuster, J., Santalla, M.C., Llases, M.E., Morgada, M.N., Vila, A.J., et al. "Fine Tuning of Functional Features of the Cu A Site by Loop-Directed Mutagenesis" . Inorganic Chemistry 58, no. 3 (2019) : 2149-2157.
http://dx.doi.org/10.1021/acs.inorgchem.8b03244
---------- MLA ----------
Zitare, U.A., Szuster, J., Santalla, M.C., Llases, M.E., Morgada, M.N., Vila, A.J., et al. "Fine Tuning of Functional Features of the Cu A Site by Loop-Directed Mutagenesis" . Inorganic Chemistry, vol. 58, no. 3, 2019, pp. 2149-2157.
http://dx.doi.org/10.1021/acs.inorgchem.8b03244
---------- VANCOUVER ----------
Zitare, U.A., Szuster, J., Santalla, M.C., Llases, M.E., Morgada, M.N., Vila, A.J., et al. Fine Tuning of Functional Features of the Cu A Site by Loop-Directed Mutagenesis. Inorg. Chem. 2019;58(3):2149-2157.
http://dx.doi.org/10.1021/acs.inorgchem.8b03244