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Abstract:

1. 1. It has been reported that hexachlorobenzene (HCB) in vivo produces hepatic porphyrinogen carboxy-lyase (PCL) decrease and pentachlorophenol (PCP) is one of its metabolites. In order to investigate if such decrease is due to an enzyme inhibitor present in the porphyric livers, the effects of the following additions on the normal rat liver PCL activity were investigated: (a) a porphyric liver preparation (free of porphyrins), (b) a heat deproteinized porphyric liver preparation, (c) HCB, PCP, several chloro and nitrophenols and other related compounds. 2. 2. The results of (a) and (b) would indicate that in porphyric liver there is probably an inhibitor tightly bound to the "porphyric" enzyme which is not separated by Sephadex G-25 and that produces an inhibition of "normal" enzyme smaller than that observed in vivo for HCB, the difference being perhaps due to a protein synthesis level action. 3. 3. From the results of (c) it is observed that (i) there are several drugs that inhibit hepatic PCL in different degrees, PCP, tetrachlorophenol and 2,4-dinitrophenol being the most effective, while HCB has no effect at any of the assayed concentrations, (ii) the inhibitory effect of these compounds could be mainly due to the presence of phenolic group and increased by the presence of electrophilic groups in the benzene ring, (iii) the above mentioned inhibitor made evident by the heating assays could be PCP, HCB being discarded because the absence of effect. However, the marked inhibitory effect of PCP decreases and disappears at lower concentrations. 4. 4. The physiological role of PCP in the production mechanism of HCB porphyria is discussed. © 1980.

Registro:

Documento: Artículo
Título:Investigations on the presence of porphyrinogen carboxy-lyase inhibitor in the liver of rats intoxicated with hexachlorobenzene
Autor:De Molina, M.D.C.R.; De Calmanovici, R.W.; De Viale, L.C.S.M.
Filiación:Laboratorio de Porfirinas, Departamento de Quimica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Núñez, 1428 Buenos Aires, Argentina
Palabras clave:2,4 dinitrophenol; hexachlorobenzene; pentachlorophenol; animal experiment; drug identification; drug induced disease; enzyme inhibition; hepatic porphyria; liver; oral drug administration; porphyrinogen carboxylase; preliminary communication; rat; tetrachlorophenol; Animal; Benzene; Biotransformation; Carboxy-Lyases; Chorionic Gonadotropin; Coproporphyrinogens; Female; Hexachlorobenzene; Liver; Pentachlorophenol; Porphyrinogens; Rats; Structure-Activity Relationship; Support, Non-U.S. Gov't
Año:1980
Volumen:12
Número:5-6
Página de inicio:1027
Página de fin:1032
DOI: http://dx.doi.org/10.1016/0020-711X(80)90206-2
Título revista:International Journal of Biochemistry
Título revista abreviado:Int. J. Biochem.
ISSN:0020711X
CODEN:IJBOB
CAS:2,4 dinitrophenol, 25550-58-7, 51-28-5; hexachlorobenzene, 118-74-1, 55600-34-5; pentachlorophenol, 87-86-5; Benzene, 71-43-2; Carboxy-Lyases, EC 4.1.1.; Chorionic Gonadotropin; Coproporphyrinogens; Hexachlorobenzene, 118-74-1; Pentachlorophenol, 87-86-5; porphyrinogen carboxy-lyase, EC 4.1.1.-; Porphyrinogens
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v12_n5-6_p1027_DeMolina

Referencias:

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  • Elder, Evans, Matlin, The porphyrinogenic action of polychlorinated hydrocarbons (1975) First International Porphyrin Meeting on Porphyrins in Human Diseases, pp. 424-431. , M. Doss, Karger, Basel
  • Elder, Evans, Matlin, The effect of the porphyrogenic compound hexachlorobenzene, on the activity of heptatic uroporphyrinogen decarboxylase in the rat (1976) Clin. Sci. molec. M ed., 51, pp. 71-80
  • Haley, Human poisoning with pentachlorophenol and its treatment (1977) Ecotoxicology and Environmental Safety, 50, pp. 343-347
  • Koss, Koransky, Steinbach, Studies on the toxicology of hexachlorobenzene II. Identification and determination of metabolites (1976) Arch Tax., 35, pp. 107-114
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  • Rios De Molina M. Del, Taira, San Martin De Viale, Preparación y propiedades de la ferroquelatasa de higado de ratas normales y con porfiria experimental (1977) 13th Natn. Meet. Argent. Soc. Biochem. Invest., , Abstr. 49
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  • San Martin De Viale, Rios De Molina M. Del, Wainstok De Calmanovici, Tomio, Experimental porphyria induced in rats by hexachlorobenzene. Studies on the stepwise decarboxylation of uroporphyrinogen and phyriaporphyrinogen in vivo and in vitro in several tissues (1975) First International Porphyrin Meeting on Porphyrins in Human Diseases, pp. 445-452. , M. Doss, Karger, Basel
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Citas:

---------- APA ----------
De Molina, M.D.C.R., De Calmanovici, R.W. & De Viale, L.C.S.M. (1980) . Investigations on the presence of porphyrinogen carboxy-lyase inhibitor in the liver of rats intoxicated with hexachlorobenzene. International Journal of Biochemistry, 12(5-6), 1027-1032.
http://dx.doi.org/10.1016/0020-711X(80)90206-2
---------- CHICAGO ----------
De Molina, M.D.C.R., De Calmanovici, R.W., De Viale, L.C.S.M. "Investigations on the presence of porphyrinogen carboxy-lyase inhibitor in the liver of rats intoxicated with hexachlorobenzene" . International Journal of Biochemistry 12, no. 5-6 (1980) : 1027-1032.
http://dx.doi.org/10.1016/0020-711X(80)90206-2
---------- MLA ----------
De Molina, M.D.C.R., De Calmanovici, R.W., De Viale, L.C.S.M. "Investigations on the presence of porphyrinogen carboxy-lyase inhibitor in the liver of rats intoxicated with hexachlorobenzene" . International Journal of Biochemistry, vol. 12, no. 5-6, 1980, pp. 1027-1032.
http://dx.doi.org/10.1016/0020-711X(80)90206-2
---------- VANCOUVER ----------
De Molina, M.D.C.R., De Calmanovici, R.W., De Viale, L.C.S.M. Investigations on the presence of porphyrinogen carboxy-lyase inhibitor in the liver of rats intoxicated with hexachlorobenzene. Int. J. Biochem. 1980;12(5-6):1027-1032.
http://dx.doi.org/10.1016/0020-711X(80)90206-2