The proposed method for the detection of organic compounds on paper chromatograms with high recovery of the sample is based on the appearance of dark spots on the chromatogram after heating the paper with, for example, the hot-plate of a domestic iron. The sensitivity of the method could be increased by examinatin under UV radiation at 366mm. This extends the detection limits to the nanomole level for many substances. The application of the method gave high recoveries of radioactive glucose and lysie. With glucose it was further found that the chemical properties of the compound were preserved. The procedure is simple and can be carried out in a few seconds without reagents, using inexpensive equipment. © 1982.
Documento: | Artículo |
Título: | Method for the detection of organic compounds on paper chromatograms with high recovery of the sample |
Autor: | Alperin, D.M.; Idoyaga-Vargas, V.P.; Carminatti, H. |
Filiación: | Instituto de Investigaciones Bioquimicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Obligado 2490, 1428 Buenos Aires, Argentina |
Año: | 1982 |
Volumen: | 242 |
Número: | 2 |
Página de inicio: | 299 |
Página de fin: | 304 |
DOI: | http://dx.doi.org/10.1016/S0021-9673(00)81704-5 |
Título revista: | Journal of Chromatography A |
Título revista abreviado: | J. Chromatogr. A |
ISSN: | 00219673 |
CODEN: | JCRAE |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219673_v242_n2_p299_Alperin |