Artículo

Russi, N.B.; Maito, J.; Dieser, S.A.; Renna, M.S.; Signorini, M.L.; Camussone, C.; Neder, V.E.; Pol, M.; Tirante, L.; Odierno, L.M.; Calvinho, L.F. "Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections" (2015) Journal of Dairy Research. 82(3):317-321
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Abstract:

Few studies have described the relationship between genotypic and phenotypic methods for detecting penicillin resistance in Staphylococcus aureus isolated from bovine intramammary infection (IMI). Six phenotypic methods for penicillinase detection were compared with a genotypic method testing the presence of the β-lactamase gene blaZ in Staph. aureus (n = 150) isolated from bovine IMI. Highest sensitivities and specificities were observed for disk diffusion (DD) (93 and 97·4%), minimum inhibitory concentration (MIC) (90·3 and 97·4%), Cefinase™ (85·9 and 97·4%) and Diatabs™ (85·7 and 98·7%). The estimated cut-off points estimated in the present study can be considered close to the ones indicated by CLSI (2013). The molecular detection of blaZ gene is the only method that may indicate the real or potential capacity of producing β-lactamase in Staph. aureus. Considering that from a clinical standpoint a false negative result from a phenotypic test is the most unfavourable situation, a combination of standard DD with Diatabs™ or Cefinase™ should be performed by routine mastitis laboratories to minimise false negative results. © 2015 Proprietors of Journal of Dairy Research.

Registro:

Documento: Artículo
Título:Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections
Autor:Russi, N.B.; Maito, J.; Dieser, S.A.; Renna, M.S.; Signorini, M.L.; Camussone, C.; Neder, V.E.; Pol, M.; Tirante, L.; Odierno, L.M.; Calvinho, L.F.
Filiación:Facultad de Ciencias Veterinarias, Universidad Nacional Del Litoral, Rvdo. Padre Kreder 2805, Esperanza, Santa Fe, Argentina
Lactodiagnóstico Sur., Pedro Goyena 3199, Olivos, Buenos Aires, Argentina
Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Rió Cuarto, Ruta 36 Km601, Rió Cuarto, Córdoba, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas, Avda. Rivadavia 1917, Ciudad Autónoma de Buenos Aires, Argentina
Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnologiá Agropecuaria, Ruta 34, Rafaela, Santa Fe, Argentina
Palabras clave:blaZ; intramammary infections; penicillin resistance; Staphylococcus aureus; Bovinae; Staphylococcus aureus; bacterial DNA; beta lactamase; penicillin G; penicillinase; animal; bovine; bovine mastitis; comparative study; drug effects; enzymology; female; genetics; genotype; laboratory diagnosis; microbial sensitivity test; microbiology; penicillin resistance; phenotype; Staphylococcus aureus; Staphylococcus infection; veterinary; Animals; beta-Lactamases; Cattle; DNA, Bacterial; False Negative Reactions; Female; Genotype; Mastitis, Bovine; Microbial Sensitivity Tests; Penicillin G; Penicillin Resistance; Penicillinase; Phenotype; Staphylococcal Infections; Staphylococcus aureus
Año:2015
Volumen:82
Número:3
Página de inicio:317
Página de fin:321
DOI: http://dx.doi.org/10.1017/S0022029915000242
Título revista:Journal of Dairy Research
Título revista abreviado:J. Diary Res.
ISSN:00220299
CODEN:JDRSA
CAS:beta lactamase, 9073-60-3; penicillin G, 1406-05-9, 61-33-6; penicillinase, 9001-74-5; beta-Lactamases; DNA, Bacterial; Penicillin G; Penicillinase
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220299_v82_n3_p317_Russi

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Citas:

---------- APA ----------
Russi, N.B., Maito, J., Dieser, S.A., Renna, M.S., Signorini, M.L., Camussone, C., Neder, V.E.,..., Calvinho, L.F. (2015) . Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections. Journal of Dairy Research, 82(3), 317-321.
http://dx.doi.org/10.1017/S0022029915000242
---------- CHICAGO ----------
Russi, N.B., Maito, J., Dieser, S.A., Renna, M.S., Signorini, M.L., Camussone, C., et al. "Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections" . Journal of Dairy Research 82, no. 3 (2015) : 317-321.
http://dx.doi.org/10.1017/S0022029915000242
---------- MLA ----------
Russi, N.B., Maito, J., Dieser, S.A., Renna, M.S., Signorini, M.L., Camussone, C., et al. "Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections" . Journal of Dairy Research, vol. 82, no. 3, 2015, pp. 317-321.
http://dx.doi.org/10.1017/S0022029915000242
---------- VANCOUVER ----------
Russi, N.B., Maito, J., Dieser, S.A., Renna, M.S., Signorini, M.L., Camussone, C., et al. Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections. J. Diary Res. 2015;82(3):317-321.
http://dx.doi.org/10.1017/S0022029915000242