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Abstract:

Haematophagous insects suffer big changes in water needs under different levels of starvation. Rhodnius prolixus is the most important haematophagous vector of Chagas disease in the north of South America and a model organism in insect physiology. Although there have been some studies on patterns of gas exchange and metabolic rates, there is little information regarding water loss in R. prolixus. We investigated whether there is any modulation of water loss and metabolic rate under different requirements for saving water. We measured simultaneously CO2 production, water emission and activity in individual insects in real time by open-flow respirometry at different temperatures (15, 25 and 35°C) and post-feeding days (0, 5, 13 and 29). We found: (1) a clear drop in metabolic rate between 5 and 13 days after feeding that cannot be explained by activity and (2) a decrease in water loss rate with increasing starvation level, by a decrease in cuticular water loss during the first 5 days after feeding and a drop in the respiratory component thereafter. We calculated the surface area of the insects and estimated cuticular permeability. In addition, we analysed the pattern of gas exchange; the change from a cyclic to a continuous pattern was affected by temperature and activity, but it was not affected by the level of starvation. Modulation of metabolic and water loss rates with temperature and starvation could help R. prolixus to be more flexible in tolerating different periods of starvation, which is adaptive in a changing environment with the uncertainty of finding a suitable host. © 2014. Published by The Company of Biologists Ltd.

Registro:

Documento: Artículo
Título:Metabolism and water loss rate of the haematophagous insect Rhodnius prolixus: Effect of starvation and temperature
Autor:Rolandi, C.; Iglesias, M.S.; Schilman, P.E.
Filiación:Departamento de Biodiversidad Y Biología Experimental, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, C1428EHA, Argentina
Instituto de Biodiversidad Y Biología Experimental Y Aplicada (IBBEA), CONICET-UBA, Buenos Aires, C1428EHA, Argentina
Palabras clave:CO2 emission rate; Cuticular permeability; Flow-through respirometry; Respiratory water loss; Hexapoda; Rhodnius prolixus; carbon dioxide; water; animal; energy metabolism; metabolism; permeability; physiological stress; physiology; Rhodnius; starvation; temperature; thermoregulation; Animals; Carbon Dioxide; Energy Metabolism; Permeability; Rhodnius; Starvation; Stress, Physiological; Temperature; Water; Water Loss, Insensible
Año:2014
Volumen:217
Número:24
Página de inicio:4414
Página de fin:4422
DOI: http://dx.doi.org/10.1242/jeb.109298
Título revista:Journal of Experimental Biology
Título revista abreviado:J. Exp. Biol.
ISSN:00220949
CODEN:JEBIA
CAS:carbon dioxide, 124-38-9, 58561-67-4; water, 7732-18-5; Carbon Dioxide; Water
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v217_n24_p4414_Rolandi

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Citas:

---------- APA ----------
Rolandi, C., Iglesias, M.S. & Schilman, P.E. (2014) . Metabolism and water loss rate of the haematophagous insect Rhodnius prolixus: Effect of starvation and temperature. Journal of Experimental Biology, 217(24), 4414-4422.
http://dx.doi.org/10.1242/jeb.109298
---------- CHICAGO ----------
Rolandi, C., Iglesias, M.S., Schilman, P.E. "Metabolism and water loss rate of the haematophagous insect Rhodnius prolixus: Effect of starvation and temperature" . Journal of Experimental Biology 217, no. 24 (2014) : 4414-4422.
http://dx.doi.org/10.1242/jeb.109298
---------- MLA ----------
Rolandi, C., Iglesias, M.S., Schilman, P.E. "Metabolism and water loss rate of the haematophagous insect Rhodnius prolixus: Effect of starvation and temperature" . Journal of Experimental Biology, vol. 217, no. 24, 2014, pp. 4414-4422.
http://dx.doi.org/10.1242/jeb.109298
---------- VANCOUVER ----------
Rolandi, C., Iglesias, M.S., Schilman, P.E. Metabolism and water loss rate of the haematophagous insect Rhodnius prolixus: Effect of starvation and temperature. J. Exp. Biol. 2014;217(24):4414-4422.
http://dx.doi.org/10.1242/jeb.109298