Artículo
Abstract:
Agrobacterium tumefaciens exoB mutants (deficient in succinoglycan or EPS I synthesis) were characterized. The defect was determined to be the lack of UDP-glucose 4-epimerase (EC 5.1.3.2) activity. This defect results in absence of UDP-galactose, which is the obligatory intermediate as galactose donor for initiation of the succinoglycan repeating unit on a lipid intermediate. It was determined that A. tumefaciens does not have the Leloir pathway enzyme activities galactokinase (EC 2.7.1.6) and galactose-1-phosphate uridylyltransferase (EC 2.7.7.10). A De Ley-Doudoroff oxidative pathway for galactose metabolism was observed in A. tumefaciens. This pathway is not constitutive and its expression required D-galactose or L-arabinose. However, UDP-glucose 4-epimerase is constitutive and did not require growth on galactose for full expression.
Registro:
| Documento: | Artículo |
| Título: | Galactose metabolism in rhizobiaceae: Characterization of agrobacterium tumefaciens exoB mutants |
| Autor: | Uttaro, A.D.; Ielpi, L.; Ugalde, R.A. |
| Filiación: | Inst Invest Bioquimica Fund Campomar, Facultad Ciencias Exactas Nat y and, CONICET, Antonio Machado 151, 1405 Buenos Aires, Argentina |
| Palabras clave: | arabinose; galactokinase; galactose; galactose 1 phosphate uridylyltransferase; glycan derivative; lipid; uridine diphosphate galactose; uridine diphosphate glucose 4 epimerase; aerobic metabolism; article; bacterium mutant; enzyme activity; enzyme metabolism; enzyme synthesis; nonhuman; priority journal; Rhizobium radiobacter; Agrobacterium tumefaciens; Bacteria (microorganisms); Rhizobiaceae |
| Año: | 1993 |
| Volumen: | 139 |
| Número: | 5 |
| Página de inicio: | 1055 |
| Página de fin: | 1062 |
| DOI: | http://dx.doi.org/10.1099/00221287-139-5-1055 |
| Título revista: | Journal of General Microbiology |
| Título revista abreviado: | J. GEN. MICROBIOL. |
| ISSN: | 00221287 |
| CODEN: | JGMIA |
| CAS: | arabinose, 147-81-9; galactokinase, 9030-53-9; galactose, 26566-61-0, 50855-33-9, 59-23-4; galactose 1 phosphate uridylyltransferase, 9016-11-9; lipid, 66455-18-3; uridine diphosphate galactose, 2956-16-3; uridine diphosphate glucose 4 epimerase, 9032-89-7 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221287_v139_n5_p1055_Uttaro |
Citas:
---------- APA ----------
Uttaro, A.D., Ielpi, L. & Ugalde, R.A. (1993) . Galactose metabolism in rhizobiaceae: Characterization of agrobacterium tumefaciens exoB mutants. Journal of General Microbiology, 139(5), 1055-1062.http://dx.doi.org/10.1099/00221287-139-5-1055
---------- CHICAGO ----------
Uttaro, A.D., Ielpi, L., Ugalde, R.A. "Galactose metabolism in rhizobiaceae: Characterization of agrobacterium tumefaciens exoB mutants" . Journal of General Microbiology 139, no. 5 (1993) : 1055-1062.http://dx.doi.org/10.1099/00221287-139-5-1055
---------- MLA ----------
Uttaro, A.D., Ielpi, L., Ugalde, R.A. "Galactose metabolism in rhizobiaceae: Characterization of agrobacterium tumefaciens exoB mutants" . Journal of General Microbiology, vol. 139, no. 5, 1993, pp. 1055-1062.http://dx.doi.org/10.1099/00221287-139-5-1055
---------- VANCOUVER ----------
Uttaro, A.D., Ielpi, L., Ugalde, R.A. Galactose metabolism in rhizobiaceae: Characterization of agrobacterium tumefaciens exoB mutants. J. GEN. MICROBIOL. 1993;139(5):1055-1062.http://dx.doi.org/10.1099/00221287-139-5-1055
