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Abstract:

Small subunit rDNA sequences of 42 taxa belonging to 10 genera were used to infer phylogenetic relationships among euglenoids. Members of the phototrophic genera Euglena, Phacus, Lepocinclis, Colacium, Trachelomonas, and Strombomonas plus the osmotrophs Astasia longa, Khawkinea quartana, and Hyalophacus ocellatus were included. Six major clades were found in most trees using multiple methods. The utility of Bayesian analyses in resolving these clades is demonstrated. The genus Phacus was polyphyletic with taxa sorting into two main clades. The two clades correlated with overall morphology and corresponded in large part to the previously defined sections, Pleuraspis Pochmann and Proterophacus Pochmann. Euglena was also polyphyletic and split into two clades. In Bayesian analyses species with less plastic pellicles and small disk-like chloroplasts diverged at the base of the tree. They grouped into a single clade which included the two Lepocinclis spp., which also are rigid and bear similar chloroplasts. The metabolic Euglena species with larger plastids bearing pyrenoids and paramylon caps arose near the top of the tree. The loricates Strombomonas and Trachelomonas formed two well-supported, but paraphyletic, clades. The strong support for the individual clades confirmed the value of using lorica features as taxonomic criteria. The separation of the osmotrophic species A. longa, K. quartana, and H. ocellatus into different clades suggested that the loss of the photosynthetic ability has occurred multiple times.

Registro:

Documento: Artículo
Título:Phylogeny of Euglenophyceae based on small subunit rDNA sequences: Taxonomic implications
Autor:Nudelman, M.A.; Rossi, M.S.; Conforti, V.; Triemer, R.E.
Filiación:Pabellón II, Ciudad Universitaria, Buenos Aires, C1428EHA, Argentina
Michigan State University, Department of Plant Biology, 166 Plant Biology, East Lansing, MI 48824-1312, United States
Palabras clave:Euglenoids; Euglenophyta; Euglenozoa; Molecular phylogeny; SSU rDNA; flagellate; phylogeny; taxonomy; algae; Astasia; Colacium; Euglena; Euglena longa; Euglenida; Euglenozoa; Eukaryota; Hyalophacus ocellatus; Khawkinea quartana; Lepocinclis; Lorica; Mastigophora (flagellates); Phacus; Strombomonas; Trachelomonas
Año:2003
Volumen:39
Número:1
Página de inicio:226
Página de fin:235
DOI: http://dx.doi.org/10.1046/j.1529-8817.2003.02075.x
Título revista:Journal of Phycology
Título revista abreviado:J. Phycol.
ISSN:00223646
CODEN:JPYLA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223646_v39_n1_p226_Nudelman

Referencias:

  • Bricheux, G., Brugerolle, G., The membrane cytoskeleton complex of Euglenids. I. Biochemical and immunological characterization of the epiplasmic proteins of Euglena gracilis (1986) Eur. J. Cell Biol., 40, pp. 150-159
  • Bricheux, G., Brugerolle, G., The pellicular complex of Euglenoids. II. A biochemical and immunological comparative study of major epiplasmic proteins (1987) Protoplasma, 140, pp. 43-54
  • Busse, I., Preisfeld, A., Phylogenetic position of Rhynchopus sp. and Diplonema ambulator as indicated by analyses of euglenozoan SSU rDNA (2002) Gene, 284, pp. 83-91
  • Conrad, W., Materiaux pour une monographie du genre Lepocinclis Perty (1934) Arch. Protkde., 82, pp. 203-249
  • Dawson, N.S., Dunlap, J.R., Walne, P.L., Structure and elemental composition of pellicular warts of Euglena spirogyra (Euglenophyceae) (1988) Br. Phycol. J., 23, pp. 61-69
  • Deflandre, G., Strombomonas nouveau genre d' Euglenacées. (Trachelomonas Ehr. p. p.) (1930) Arch. Protkde., 69, pp. 551-614
  • Donnelly Barnes, L.S., Walne, P.L., Dunlap, J.R., Cytological and taxonomic studies of the Euglenales. I. Ultrastructure and envelope composition in Trachelomonas spp. (1986) Br. Phycol. J., 21, pp. 387-397
  • Dujardin, F., (1841) Histoire Naturelle des Zoophytes Infusoires, , Roret, Paris, 684 pp
  • Dunlap, J.R., Walne, P.L., Variations in envelope morphology and mineralization in Trachelomonas lefevrei (Euglenophyceae) (1987) J. Phycol., 23, pp. 556-564
  • Dunlap, J.R., Walne, P.L., Bentley, J., Microarchitecture and elemental spatial aggregation of envelopes of Trachelomonas lefevrei (Euglenophyceae) (1983) Protoplasma, 117, pp. 97-106
  • Dunlap, J.R., Walne, P.L., Kivic, P.A., Cytological and taxonomic studies of the Euglenales. II. Comparative microarchitecture and cytochemistry of envelopes of Strombomonas and Trachelomonas (1986) Br. Phycol. J., 21, pp. 399-405
  • Dynesius, R.A., Walne, P.L., Ultrastructure of the reservoir and flagella in Phacus pleuronectes (Euglenophyceae) (1975) J. Phycol., 11, pp. 125-130
  • Ehrenberg, C.G., (1838) Die Infusionsthierchen als Vollkommene Organismen, , Verlag von Leopold Voss, Leipzig, 547 pp
  • Felsenstein, J., Confidence limits on phylogenies: An approach using the bootstrap (1985) Evolution, 39, pp. 783-791
  • Gibbs, S.P., The chloroplasts of Euglena may have evolved from symbiotic green algae (1978) Can. J. Bot., 56, pp. 2883-2889
  • Gibbs, S.P., The chloroplasts of some algae groups may have evolved from endosymbiotic eukaryotic algae (1981) Ann. N. Y. Acad. Sci., 361, pp. 193-208
  • Godjics, M., (1953) The Genus Euglena, , The University of Wisconsin Press, Madison, 268 pp
  • Hall, B.G., (2001) Phylogenetic Trees Made Easy, , Sinauer Associates, Sunderland, MA, 179 pp
  • Hillis, D.M., Huelsenbeck, J.P., Signal, noise, and reliability in molecular phylogenetic analyses (1992) J. Hered., 83, pp. 189-195
  • Hüber-Pestalozzi, G., Das phytoplankton des süsswassers. Systematic und biologie. Vol. 4. Euglenophyceae (1955) Die Binnengewässer. E. Schweizerbartsche Verlagsbuchandlung, 4. , Thienemann, A. [Ed.]. Nägele und Obermiller, Stuttgart, 1135 pp
  • Huelsenbeck, J.P., Ronquist, F., MRBAYES: Bayesian inference of phylogenetic trees (2001) Bioinformatics, 17, pp. 754-755
  • Kim, J.T., Boo, S.M., Coute, A., Taxonomic and floristic accounts of the genus Trachelomonas Ehr. 1833 from Korea (2000) Korean J. Limnol., 33, pp. 80-108
  • Kivic, P.A., Walne, P.L., An evaluation of a possible phylogenetic relationship between Euglenophyta and Kinetoplastida (1984) Origin Life, 13, pp. 269-288
  • Kjer, K.M., Use of rRNA secondary structure in phylogenetic studies to identify homologous positions: An example of alignment and data presentation from the frogs (1995) Mol. Phylo. Evol., 4, pp. 314-330
  • Leander, B.S., Farmer, M.A., Evolution of Phacus (Euglenozoa) as inferred from pellicle morphology and small subunit rDNA (2001) J. Phycol., 37, pp. 143-159
  • Leander, B.S., Farmer, M.A., Comparative morphology of the euglenid pellicle. II (2001) J. Eukaryot. Microbiol., 48, pp. 204-217
  • Leander, B.S., Witek, R.P., Farmer, M.A., Trends in the evolution of the euglenoid pellicle (2001) Evolution, 55, pp. 2215-2235
  • Leedale, G.F., Pellicle structure in Euglena (1964) Br. Phycol. Bull., 2, pp. 291-306
  • Leedale, G.F., (1967) Euglenoid Flagellates, , Prentice Hall, Englewood Cliffs, NJ, 242 pp
  • Lemmermann, E., Kryptogamenflora der Mark Brandenburg und angrenzender Geiviete (herausgegeben von dem Bot. Ver. der Prov. Brandenburg) (1910) Algen I (Schizophyceen, Flagellaten, Peridineen). Eugleninen, 3, pp. 484-562. , Borntraeger, Leipzig
  • Linton, E.W., Hittner, H., Lewandowski, C., Auld, T., Triemer, R.E., A molecular study of Euglenoid phylogeny using small subunit rDNA (1999) J. Euk. Microbiol., 46, pp. 217-223
  • Linton, E.W., Nudelman, M.A., Conforti, V., Triemer, R.E., A molecular analysis of the euglenophytes using SSU rDNA (2000) J. Phycol., 36, pp. 740-746
  • Maslov, D.A., Yasuhira, S., Simpson, L., Phylogenetic affinities of Diplonema within the Euglenozoa as inferred from the SSU rRNA gene and partial COX I protein sequences (1999) Protist, 150, pp. 33-42
  • Mignot, J.P., Ultrastructure des eugléniens 1. Étude de la cuticule chez différentes espécies (1965) Protistologica, 1, pp. 5-15
  • Milanowski, R., Zakrys, B., Kwiatowski, J., Phylogenetic analysis of chloroplast small subunit rRNA genes of the genus Euglena Ehrenberg (2001) IJSEM, 51, pp. 773-781
  • Montegut-Felkner, A.E., Triemer, R.E., Phylogenetic relationships of select euglenoid genera based on morphological and molecular data (1997) J. Phycol., 33, pp. 512-519
  • Muellner, A.N., Angeler, D.G., Samuel, R., Linton, E.W., Triemer, R.E., Phylogenetic analysis of phagotrophic, phototrophic and osmotrophic euglenoids by using the nuclear 18S rDNA sequence (2001) IJSEM, 51, pp. 783-791
  • Nudelman, M.A., Conforti, V., Trachelomonas argentinensis (Euglenophyta) nov. comb (1997) Bol. Soc. Arg. Bot., 33, pp. 17-22
  • Nudelman, M.A., Lombardo, R., Conforti, V., Comparative analysis of envelopes of Trachelomonas argentinensis (Euglenophyta) from different aquatic environments in South America (1998) Arch. Hydrobiol. Algol. Stud., 89, pp. 97-105
  • Owens, K.J., Farmer, M.A., Triemer, R.E., The flagellar apparatus and reservoir/cytoskeleton of Cryptoglena pigra (Euglenophyceae) (1988) J. Phycol., 24, pp. 520-528
  • Perty, M., (1852) Zur Kenntnis Kleinster Lebensformen nach Bau, Funktionen, Systematik, mit Spezialverzeichnis der in der Schweiz Beobachteten, , Jent und Reinert, Bern, 228 pp
  • Playfair, G.J., Australian freshwater flagellates (1921) Proc. Linn. Soc. N. S. Wales, Sydney, 46, pp. 99-146
  • Pochmann, A., Synopsis der gattung Phacus (1942) Arch. Protistenk., 65, pp. 81-252
  • Posada, D., Crandall, K.A., MODELTEST: Testing the model of DNA substitution (1998) Bioinformatics, 14, pp. 817-818
  • Preisfeld, A., Berger, S., Busse, I., Liller, S., Ruppel, H.G., Phylogenetic analyses of various euglenoid taxa (Euglenozoa) based on 18S rDNA sequence data (2000) J. Phycol., 36, pp. 220-226
  • Pringsheim, E.G., Observations on some species of Trachelomonas grown in culture (1953) New Phytol., 52, pp. 93-113
  • Pringsheim, E.G., Contributions towards a monograph of the genus Euglena (1956) Nova Acta Leopoldina, 125, pp. 1-168
  • Rosowski, J.R., Vadas, R.L., Kugrens, P., Surface configurations of the lorica of the Euglenoid Trachelomonas as revealed with scanning electron microscopy (1975) Am. J. Bot., 62, pp. 48-57
  • Rosowski, J.R., Walne, P.L., West, L.K., Comparative effects of critical point on air-drying on the morphology of the rigid mucilaginous coating (lorica) of Trachelomonas (Euglenophyceae) (1975) Micron, 5, pp. 221-329
  • Shin, W., Boo, S.M., Ultrastructure of Phacus trypanon (Euglenophyceae) with an emphasis on striated fiber and microtubules arrangement (2001) J. Phycol., 37, pp. 95-105
  • Shin, W., Boo, S.M., Inouye, I., Ultrastructure of Euglena anabaena var. minor (Euglenophyceae) (2000) Phycol. Res., 48, pp. 19-25
  • Shin, W., Boo, S.M., Triemer, R.E., Ultrastructure of the basal body complex and putative vestigial feeding apparatus in Phacus pleuronectes (Euglenophyceae) (2001) J. Phycol., 37, pp. 913-921
  • Singh, K.P., Studies in the genus Trachelomonas I, II. Description of six organisms grown in cultivation (1956) Am. J. Bot., 43, pp. 258-266
  • Smith, S.W., Overbeek, R., Woese, C.R., Gilbert, W., Gillevet, P.M., The genetic data environment: An expandable GUI for multiple sequence analysis (1994) Comput. Appl. Biosci., 10, pp. 671-675
  • Solomon, J.A., Walne, P.L., Dawson, N.S., Willey, R.L., Structural characterization of Eutreptiella (Euglenophyta). II. The flagellar root system and putative vestigial cytopharinx (1991) Phycologia, 30, pp. 402-414
  • Starmach, K., (1983) Flora Slodkowodna Polski, 3. Euglenophyta-Eugleniny, , Pansttwowe wydawnictwo Naukowe, Warsawa-Krakow, 594 pp
  • Surek, B., Melkonian, M., A cryptic cytostome is present in Euglena (1986) Protoplasma, 133, pp. 39-49
  • Suzaki, T., Williamson, R.E., Euglenoid movement in Euglena fusca. Evidence for sliding between pellicular strips (1985) Protoplasma, 124, pp. 137-146
  • Suzaki, T., Williamson, R.E., Pellicular ultrastructure and euglenoid movement in Euglena ehrenbergii Klebs and Euglena oxyuris Schmarda (1986) J. Protozool., 33, pp. 165-171
  • Swirenko, D.O., Zur kentis der rusichen algenflora. Die euglenaceen gattung trachelomonas (1914) Arch. Hydrobiol., 9, pp. 630-647
  • Swofford, D.L., (1999) PAUP*: Phylogenetic Analysis Using Parsimony (and Other Methods). Version 4.0, , Sinauer Associates, Sunderland, MA
  • Triemer, R.E., Farmer, M.A., The ultrastructural organization of the heterotrophic euglenids and its evolutionary implications (1991) The Biology of Free-Living Heterotrophic Flagellates, pp. 186-204. , Patterson, D.J. & Larsen, J. [Eds.]. Clarendon Press, Oxford
  • Triemer, R.E., Farmer, M.A., An ultrastructural comparison of the mitotic apparatus, feeding apparatus flagellar apparatus and cytoskeleton in euglenoids and kinetoplastids (1991) Protoplasma, 164, pp. 91-104
  • Van De Peer, Y., Robbrecht, E., De Hoog, S., Caers, A., De Rijk, P., De Wachter, R., Database on the structure of small subunit ribosomal RNA (1999) Nucleic Acids Res., 27, pp. 179-183
  • West, L.K., Walne, P.L., Trachelomonas hispida var. coronata (Euglenophyceae). II. Envelope substructure (1980) J. Phycol., 16, pp. 498-506
  • Willey, R.L., Wibel, R.G., A cytostome/cytopharinx in green euglenoids flagellates (Euglenales) and its phylogenetic implications (1985) Biosystems, 18, pp. 369-376
  • Willey, R.L., Wibel, R.G., The reservoir cytoskeleton and a possible cytostomal homologue in Colacium (Euglenophyceae) (1985) J. Phycol., 21, pp. 570-577
  • Willey, R.L., Walne, P.L., Kivic, P.A., Phagotrophy and the origin of the euglenoid flagellates (1988) CRC Crit. Rev. Plant Sci., 7, pp. 303-340
  • Zakrys, B., Contribution to the monograph of Polish members of the genus Euglena Ehrenberg 1830 (1986) Nova Hedw., 42, pp. 491-540

Citas:

---------- APA ----------
Nudelman, M.A., Rossi, M.S., Conforti, V. & Triemer, R.E. (2003) . Phylogeny of Euglenophyceae based on small subunit rDNA sequences: Taxonomic implications. Journal of Phycology, 39(1), 226-235.
http://dx.doi.org/10.1046/j.1529-8817.2003.02075.x
---------- CHICAGO ----------
Nudelman, M.A., Rossi, M.S., Conforti, V., Triemer, R.E. "Phylogeny of Euglenophyceae based on small subunit rDNA sequences: Taxonomic implications" . Journal of Phycology 39, no. 1 (2003) : 226-235.
http://dx.doi.org/10.1046/j.1529-8817.2003.02075.x
---------- MLA ----------
Nudelman, M.A., Rossi, M.S., Conforti, V., Triemer, R.E. "Phylogeny of Euglenophyceae based on small subunit rDNA sequences: Taxonomic implications" . Journal of Phycology, vol. 39, no. 1, 2003, pp. 226-235.
http://dx.doi.org/10.1046/j.1529-8817.2003.02075.x
---------- VANCOUVER ----------
Nudelman, M.A., Rossi, M.S., Conforti, V., Triemer, R.E. Phylogeny of Euglenophyceae based on small subunit rDNA sequences: Taxonomic implications. J. Phycol. 2003;39(1):226-235.
http://dx.doi.org/10.1046/j.1529-8817.2003.02075.x