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Abstract:

Bryopsis sp. from a restricted area of the rocky shore of Mar del Plata (Argentina) on the Atlantic coast was identified as Bryopsis plumosa (Hudson) C. Agardh (Bryopsidales, Chlorophyta) based on morphological characters and rbcL and tufA DNA barcodes. To analyze the cell wall polysaccharides of this seaweed, the major room temperature (B1) and 90°C (X1) water extracts were studied. By linkage analysis and NMR spectroscopy, the structure of a sulfated galactan was determined, and putative sulfated rhamnan structures and furanosidic nonsulfated arabinan structures were also found. By anion exchange chromatography of X1, a fraction (F4), comprising a sulfated galactan as major structure was isolated. Structural analysis showed a linear backbone constituted of 3-linked β-d-galactose units, partially sulfated on C-6 and partially substituted with pyruvic acid forming an acetal linked to O-4 and O-6. This galactan has common structural features with those of green seaweeds of the genus Codium (Bryopsidales, Chlorophyta), but some important differences were also found. This is the first report about the structure of the water-soluble polysaccharides biosynthesized by seaweeds of the genus Bryopsis. These sulfated galactans and rhamnans were in situ localized mostly in two layers, one close to the plasma membrane and the other close to the apoplast, leaving a middle amorphous, unstained cell wall zone. In addition, fibrillar polysaccharides, comprising (1→3)-β-d-xylans and cellulose, were obtained by treatment of the residue from the water extractions with an LiCl/DMSO solution at high temperature. These polymers were also localized in a bilayer arrangement. © 2012 Phycological Society of America.

Registro:

Documento: Artículo
Título:Characterization of cell wall polysaccharides of the coencocytic green seaweed bryopsis plumosa (bryopsidaceae, chlorophyta) from the argentine coast
Autor:Ciancia, M.; Alberghina, J.; Arata, P.X.; Benavides, H.; Leliaert, F.; Verbruggen, H.; Estevez, J.M.
Filiación:Departamento de Biología Aplicada y Alimentos (CIHIDECAR-CONICET), Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE Buenos Aires, Argentina
Departamento de Ecología Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria-Pabellón 2, 1428 Buenos Aires, Argentina
Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP), Paseo Victoria Ocampo No 1, Escollera Norte, B7602HSA Mar del Plata, Buenos Aires, Argentina
Phycology Research Group, Center for Molecular Phylogenetics and Evolution, Ghent University, Krijgslaan 281 (S8), B-9000 Gent, Belgium
Instituto de Fisiología Biología Molecular y Neurociencias (IFIBYNE-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina
The National Research Council of Argentina, CONICET, Argentina
Palabras clave:Bryopsis plumose; Cell wall; Green seaweed; Pyruvylated galactan sulfate; Sulfated polysaccharides; Bryopsidaceae; Bryopsis; Bryopsis plumosa; Caulerpales; Chlorophyta; Codium
Año:2012
Volumen:48
Número:2
Página de inicio:326
Página de fin:335
DOI: http://dx.doi.org/10.1111/j.1529-8817.2012.01131.x
Título revista:Journal of Phycology
Título revista abreviado:J. Phycol.
ISSN:00223646
CODEN:JPYLA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223646_v48_n2_p326_Ciancia

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Citas:

---------- APA ----------
Ciancia, M., Alberghina, J., Arata, P.X., Benavides, H., Leliaert, F., Verbruggen, H. & Estevez, J.M. (2012) . Characterization of cell wall polysaccharides of the coencocytic green seaweed bryopsis plumosa (bryopsidaceae, chlorophyta) from the argentine coast. Journal of Phycology, 48(2), 326-335.
http://dx.doi.org/10.1111/j.1529-8817.2012.01131.x
---------- CHICAGO ----------
Ciancia, M., Alberghina, J., Arata, P.X., Benavides, H., Leliaert, F., Verbruggen, H., et al. "Characterization of cell wall polysaccharides of the coencocytic green seaweed bryopsis plumosa (bryopsidaceae, chlorophyta) from the argentine coast" . Journal of Phycology 48, no. 2 (2012) : 326-335.
http://dx.doi.org/10.1111/j.1529-8817.2012.01131.x
---------- MLA ----------
Ciancia, M., Alberghina, J., Arata, P.X., Benavides, H., Leliaert, F., Verbruggen, H., et al. "Characterization of cell wall polysaccharides of the coencocytic green seaweed bryopsis plumosa (bryopsidaceae, chlorophyta) from the argentine coast" . Journal of Phycology, vol. 48, no. 2, 2012, pp. 326-335.
http://dx.doi.org/10.1111/j.1529-8817.2012.01131.x
---------- VANCOUVER ----------
Ciancia, M., Alberghina, J., Arata, P.X., Benavides, H., Leliaert, F., Verbruggen, H., et al. Characterization of cell wall polysaccharides of the coencocytic green seaweed bryopsis plumosa (bryopsidaceae, chlorophyta) from the argentine coast. J. Phycol. 2012;48(2):326-335.
http://dx.doi.org/10.1111/j.1529-8817.2012.01131.x