Artículo
Abstract:
A functional relationship between the neuroendocrine and immune systems has been clearly established. We examined the role of neuroendocrine changes, particularly thyrotropin-releasing hormone (TRH) and prolactin (PRL), during the T cell-dependent immune response. After immunization of rats with sheep red blood cells (SRBC, a T cell-dependent antigen) we observed: a) an increase of hypothalamic TRH mRNA at 4 to 24 h post-immunization (i.e: SRBC vs saline: 4 h, 2.8x), in contrast to the decrease of TRH mRNA observed following treatment with LPS, a T-independent antigen (LPS vs saline: 4 h, 1.6x); b) an increase in pituitary TRH receptor mRNA and plasma PRL levels but no changes in thyroid-stimulating hormone and growth hormone plasma levels. Intracerebroventricular (icv) injection in conscious freely-moving rats of antisense oligonucleotide complementary to rat TRH mRNA resulted in: a) a significant inhibition of specific antibody production [ELISA 7 days: Ig(M+G): TRH sense vs TRH-antisense: 384 ± 27 (n = 11) vs 193 ± 22 (n = 11); p < 0.001] b) an inability to produce the peak in plasma PRL levels in rats immunized with SRBC [(12h post-immunization, TRH-sense vs TRH-antisense: 8.3 ± 1.4 (n = 6) vs 2.2 ± 0.5 (n = 6); p < 0 01]; c) a decrease in hypothalamic TRH mRNA (TRH-sense vs TRH-antisense: 12h, 1.7x). These studies demonstrate that the T-cell antigen needs an early activation of TRH and PRL for an adequate immune response, in contrast to the inhibition induced by a T-cell independent antigen.
Registro:
| Documento: | Artículo |
| Título: | The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response |
| Autor: | Castro, C.P.; Pereda, M.P.; Keller, E.A.; Arzt, E. |
| Filiación: | Lab. de Fisiol. y Biol. Molecular, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina Lab. de Fisiol. y Biologia Molecular, Fac. de Ciencias Exactas y Naturales, UBA, 1428 Buenos Aires, Argentina |
| Palabras clave: | Immune modulation; Prolactin; SRBC; T-lymphocytes; TRH antisense; antisense oligonucleotide; protirelin; analysis of variance; animal; antibody production; article; blood; erythrocyte; immune system; immunology; male; metabolism; neurosecretion; rat; sheep; T lymphocyte; Wistar rat; Analysis of Variance; Animals; Antibody Formation; Erythrocytes; Immune System; Male; Neurosecretory Systems; Oligonucleotides, Antisense; Rats; Rats, Wistar; Sheep; T-Lymphocytes; Thyrotropin-Releasing Hormone |
| Año: | 1998 |
| Volumen: | 58 |
| Número: | 2 |
| Página de inicio: | 189 |
| Página de fin: | 193 |
| Título revista: | Medicina |
| Título revista abreviado: | Medicina (Argentina) |
| ISSN: | 00257680 |
| CODEN: | MEDCA |
| CAS: | Oligonucleotides, Antisense; Thyrotropin-Releasing Hormone, 24305-27-9 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v58_n2_p189_Castro |
Referencias:
- Besedovsky, H.O., Del Rey, A., Immune-neuro-endocrine interactions: Facts and hypotheses (1996) Endocr Rev, 17, pp. 64-102
- Besedovsky, H.O., Sorkin, E., Kelier, M., Muller, J., Changes in blood hormone levels during the immune response (1975) Proc Soc Exp Med, 150, pp. 466-502
- Raiden, S., Polack, E., Nahmod, V., Labeur, M., Holsboer, F., Arzt, E., TRH receptor on immune cells: In vitro and in vivo stimulation of human lymphocyte and rat splenocyle DNA synthesis by TRH (1995) J Clin Immunol, 15, pp. 242-249
- Matera, L., Endocrine, paracrine and autocrine actions of prolactin on immune cells (1996) Life Sci, 8, pp. 599-613
- Nagy, E., Berczi, I., Wren, G.E., Asa, S.L., Kovacs, K., Immunomodulation by bromocriptine (1983) Immunopharmacology, 6, pp. 231-243
- Reul, J.M.H.M., Stec, I., Wiegers, G.J., Labeur, M.S., Linthorst, A.C.E., Arzt, E., Prenatal immune challenge alters the hypothalamic-pituitary-adrenocortical axis in the adult rats (1994) J Clin Invest, 93, pp. 2600-2607
- Kakusca, I., Romero, L., Clark, B., Rondeel, J., Qi, Y., Alex, S., Suppression of thyrotropin-releasing hormone gene expression by interleukin-1β in the rat implications for nonthyroidal illes (1994) Neuroendocrinology, 59, pp. 129-137
- García, S.I., Porto, P.I., Alvarez, A.L., Shaurli, D., Finkielman, S., Pirola, C.J., Central overexpression of the TRH precursor gene induced hypertension in rats: Antisense reversal (1997) Hypertension, 30, pp. 759-766
- Bemton, E., Beach, J.E., Holaday, J.W., Smallridge, R.C., Fein, H.G., Release of multiple hormones by a direct action of interleuquin-1 on pituitary cells (1987) Science, 238, pp. 519-521
- Arzt, E., Buric, R., Stelzer, G., Stalla, J., Sauer, J., Renner, U., Interleukin involvement in anterior pituitary cell growth regulation: Effects of IL-2 and IL-6 (1993) Endocrinology, 132, pp. 459-467
- Karanth, S., McCann, S.M., Influence of dopamine on the altered release of prolactine, luteinizing hormone and follicle stimulating hormone induced by interleukin-2 in vitro (1992) Neuroendocrinology, 56, pp. 871-880
- Besedovsky, H.O., Del Rey, A., Sorkin, E., Da Prada, M., Burri, R., Honegger, C., The immune response evokes changes in brain noradrenergic neurons (1983) Science, 221, pp. 564-565
- Hickey, W.F., Hsu, B.L., Kimura, H., T-lymphocyte entry into the central nervous system (1991) J Neurosci Res, 28, pp. 254-260
- Hoang-Vu, C., Leitoif, H., Brabant, G., Continuous vs pulsatile administration of thyrotropin-releasing hormone (TRH) in the model of the chronically cannulated rat: Long-term effect on thyroid function (1996) Exp Clin Endocrinol Diabetes, 104, pp. 51-54
- Redei, E., Hllderbrand, H., Aird, F., Corticotropin release-inhibiting factor in preprothyrotropm-releasing hormone-(178-199) (1995) Endocrinology, 136, pp. 3557-3563
Citas:
---------- APA ----------
Castro, C.P., Pereda, M.P., Keller, E.A. & Arzt, E. (1998) . The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response . Medicina, 58(2), 189-193.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v58_n2_p189_Castro [ ]
---------- CHICAGO ----------
Castro, C.P., Pereda, M.P., Keller, E.A., Arzt, E. "The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response " . Medicina 58, no. 2 (1998) : 189-193.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v58_n2_p189_Castro [ ]
---------- MLA ----------
Castro, C.P., Pereda, M.P., Keller, E.A., Arzt, E. "The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response " . Medicina, vol. 58, no. 2, 1998, pp. 189-193.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v58_n2_p189_Castro [ ]
---------- VANCOUVER ----------
Castro, C.P., Pereda, M.P., Keller, E.A., Arzt, E. The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response . Medicina (Argentina). 1998;58(2):189-193.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v58_n2_p189_Castro [ ]
