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Abstract:

We previously reported the presence of serotonin (5-HT) in testes from golden hamster, a photoperiodic species which is a useful model for the study of states of male (in)fertility. The aims of this study were to investigate: (1) the presence of intrinsic sources of 5-HT in the testis; (2) the role of 5-HT in in vitro androgen production; (3) the serotoninergic receptor subtypes in the testis, and (4) the existence of interactions among the 5-HT receptors and the testicular catecholaminergic and corticotropin-releasing hormone (CRH) systems. Immunohistochemical studies revealed the presence of tryptophan hydroxylase, a 5-HT-biosynthetic enzyme, in interstitial cells which show the characteristic punctate chromatin pattern of Leydig cells. We describe an inhibitory action of 5-HT on testosterone, dihydrotestosterone, and androstane-3α,17β-diol production from testes of peripubertal and adult hamsters maintained in a long photoperiod (14/10 h light/dark), and adult animals exposed to a short photoperiod (6/18 h light/dark). By using several agonists and antagonists of 5-HT receptors, we characterized 5-HT1A and 5-HT2A receptor subtypes involved in the inhibitory action of this neurotransmitter on human chorionic gonadotropin stimulated cyclic adenosine monophosphate and testosterone production. CRH also produced a negative modulation of both parameters, but epinephrine and norepinephrine, through α1/β1-adrenergic receptors, exerted a stimulatory action. 5-HT1A, 5-HT2, and CRH antagonists showed that the testicular activity of the serotoninergic system, but also the α1/β1-adrenergic receptor system, is mediated by CRH. Moreover, interactions between the 5-HT2A receptor system and α1/β-adrenergic receptors have been established. Thus, these data suggest that α1/β1-adrenergic receptors are involved in the local regulatory action exerted by 5-HT on steroidogenesis through a 5-HT2-receptor-mediated response and the CRH system. Copyright © 2002 S. Karger AG, Basel.

Registro:

Documento: Artículo
Título:Interactions between testicular serotoninergic, catecholaminergic, and corticotropin-releasing hormone systems modulating cAMP and testosterone production in the golden hamster
Autor:Frungieri, M.B.; Zitta, K.; Pignataro, O.P.; Gonzalez-Calvar, S.I.; Calandra, R.S.
Filiación:Instituto de Biología y Medicina Experimental, CONICET, Buenos Aires, Argentina
Facultad de Medicina, UBA, Buenos Aires, Argentina
Cátedra de Endocrinología, Facultad de Ciencias Exactas, UNLP, La Plata, Argentina
Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Catecholamines; Corticotropin-releasing hormone; Gonadal steroids; Hamster; Immunocytochemistry; Serotonin; Serotonin agonists; Serotonin antagonists; Steroidogenesis; Testis; 2 dipropylamino 8 hydroxytetralin; 4 iodo 2,5 dimethoxyamphetamine; 4 iodo n [2 [4 (methoxyphenyl) 1 piperazinyl]ethyl] n 2 pyridinylbenzamide; adrenalin; alpha 1 adrenergic receptor; androstanediol; androstanolone; benzamide derivative; beta 1 adrenergic receptor; bucladesine; chorionic gonadotropin; clozapine; corticotropin releasing factor[9-41]; cyclic AMP; isoprenaline; ketanserin; metitepine; metoclopramide; noradrenalin; oxymetazoline; phenylephrine; prazosin; quipazine; serotonin; serotonin 1A receptor; serotonin 2A receptor; testosterone; tryptophan hydroxylase; unclassified drug; unindexed drug; zinterol; androgen synthesis; animal tissue; article; catecholaminergic system; chromatin; controlled study; dose response; drug effect; hamster; immunohistochemistry; Leydig cell; male; nonhuman; photoperiodicity; prepuberty; priority journal; serotoninergic system; steroidogenesis; testis; Animals; Bucladesine; Catecholamines; Chorionic Gonadotropin; Corticotropin-Releasing Hormone; Cricetinae; Cyclic AMP; Drug Interactions; Epinephrine; Immunohistochemistry; Leydig Cells; Male; Mesocricetus; Norepinephrine; Peptide Fragments; Photoperiod; Receptor, Serotonin, 5-HT2A; Receptors, Adrenergic, alpha-1; Receptors, Adrenergic, beta-1; Receptors, Serotonin; Serotonin; Serotonin Antagonists; Testis; Testosterone; Tryptophan Hydroxylase
Año:2002
Volumen:76
Número:1
Página de inicio:35
Página de fin:46
DOI: http://dx.doi.org/10.1159/000063682
Título revista:Neuroendocrinology
Título revista abreviado:Neuroendocrinology
ISSN:00283835
CODEN:NUNDA
CAS:Bucladesine, 362-74-3; Catecholamines; Chorionic Gonadotropin; corticotropin releasing hormone (9-41), 96118-75-1; Corticotropin-Releasing Hormone, 9015-71-8; Cyclic AMP, 60-92-4; Epinephrine, 51-43-4; Norepinephrine, 51-41-2; Peptide Fragments; Receptor, Serotonin, 5-HT2A; Receptors, Adrenergic, alpha-1; Receptors, Adrenergic, beta-1; Receptors, Serotonin; Serotonin Antagonists; Serotonin, 50-67-9; Testosterone, 58-22-0; Tryptophan Hydroxylase, EC 1.14.16.4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v76_n1_p35_Frungieri

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Citas:

---------- APA ----------
Frungieri, M.B., Zitta, K., Pignataro, O.P., Gonzalez-Calvar, S.I. & Calandra, R.S. (2002) . Interactions between testicular serotoninergic, catecholaminergic, and corticotropin-releasing hormone systems modulating cAMP and testosterone production in the golden hamster. Neuroendocrinology, 76(1), 35-46.
http://dx.doi.org/10.1159/000063682
---------- CHICAGO ----------
Frungieri, M.B., Zitta, K., Pignataro, O.P., Gonzalez-Calvar, S.I., Calandra, R.S. "Interactions between testicular serotoninergic, catecholaminergic, and corticotropin-releasing hormone systems modulating cAMP and testosterone production in the golden hamster" . Neuroendocrinology 76, no. 1 (2002) : 35-46.
http://dx.doi.org/10.1159/000063682
---------- MLA ----------
Frungieri, M.B., Zitta, K., Pignataro, O.P., Gonzalez-Calvar, S.I., Calandra, R.S. "Interactions between testicular serotoninergic, catecholaminergic, and corticotropin-releasing hormone systems modulating cAMP and testosterone production in the golden hamster" . Neuroendocrinology, vol. 76, no. 1, 2002, pp. 35-46.
http://dx.doi.org/10.1159/000063682
---------- VANCOUVER ----------
Frungieri, M.B., Zitta, K., Pignataro, O.P., Gonzalez-Calvar, S.I., Calandra, R.S. Interactions between testicular serotoninergic, catecholaminergic, and corticotropin-releasing hormone systems modulating cAMP and testosterone production in the golden hamster. Neuroendocrinology. 2002;76(1):35-46.
http://dx.doi.org/10.1159/000063682