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Abstract:

The effect of copper-imposed oxidative stress on isocitrate lyase (ICL) activity was studied in a Chlamydomonas reinhardtii wild type and a copper-tolerant pleiotropic mutant strain. Suspension batch bioassays were performed adding copper chloride under moderate irradiation. Two harvest points were selected to represent the acute and restoration phases and oxidative stress indicators were measured: antioxidative metabolites, phenolic compounds, ascorbic acid, abundance of thiobarbituric reactive substances, and activities of detoxifying enzymes such as catalase and ascorbate peroxidase. Copper stress under moderate irradiance favours acetate assimilation through enhanced ICL activity, especially in the acute phase. Though the mutant strain exhibited at least twofold ICL activity in the acute phase compared to the wild type, there was no significant difference in copper tolerance between strains. The higher ICL activity under copper stress may be an adaptation to promote mixotrophic organic carbon assimilation when photosynthetic carbon dioxide fixation is impaired. Thus, the higher ICL activity has the potential to be a useful indicator in acetate-flagellate bioassays.

Registro:

Documento: Artículo
Título:Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)
Autor:Sztrum, A.A.; Sabatini, S.E.; Rodríguez, M.C.
Filiación:Instituto de Ciencias Ambientales y Salud, Fundación PROSAMA, Paysandú 752 (1405), Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917, 1033 Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, 1428 Buenos Aires, Argentina
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales and CIHIDECAR (CONICET), Universidad de Buenos Aires, Pabellón 2, 1428 Buenos Aires, Argentina
Palabras clave:Ascorbate peroxidase; Catalase; Chlamydomonas reinhardtii; Copper; Isocitrate lyase; Lipid peroxidation; Oxidative stress; Chlamydomonadales; Chlamydomonas reinhardtii; Chlorophyceae; Mastigophora (flagellates)
Año:2012
Volumen:51
Número:2
Página de inicio:135
Página de fin:143
DOI: http://dx.doi.org/10.2216/10-56.1
Título revista:Phycologia
Título revista abreviado:Phycologia
ISSN:00318884
CODEN:PYCOA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318884_v51_n2_p135_Sztrum

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Citas:

---------- APA ----------
Sztrum, A.A., Sabatini, S.E. & Rodríguez, M.C. (2012) . Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae). Phycologia, 51(2), 135-143.
http://dx.doi.org/10.2216/10-56.1
---------- CHICAGO ----------
Sztrum, A.A., Sabatini, S.E., Rodríguez, M.C. "Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)" . Phycologia 51, no. 2 (2012) : 135-143.
http://dx.doi.org/10.2216/10-56.1
---------- MLA ----------
Sztrum, A.A., Sabatini, S.E., Rodríguez, M.C. "Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)" . Phycologia, vol. 51, no. 2, 2012, pp. 135-143.
http://dx.doi.org/10.2216/10-56.1
---------- VANCOUVER ----------
Sztrum, A.A., Sabatini, S.E., Rodríguez, M.C. Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae). Phycologia. 2012;51(2):135-143.
http://dx.doi.org/10.2216/10-56.1